A STUDY ON THE INFLUENCE OF PLANT GROWTH REGULATOR ON SHOOT MULTIPLICATION AND EVALUATION OF MAJOR WITHANOLIDES IN WITHANIA somnifera

Senthil, Kalaiselvi

doi:10.20959/wjpps20178-8944

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Any alteration in the concentration of auxin or cytokinin or a change in their ratio would dramatically alter the growth pattern and metabolite accumulation (Rao & Ravishankar, 2002). Among the various concentrations and combinations of plant growth regulators studied (BAP and Kn), MS medium supplemented with 4.44μM BAP was reported with 1.86 fold increase in WA content compared to control (Murugesan. et al, 2017).…”

Section: Role Of Plant Tissue Culture In Wa Productionmentioning

confidence: 97%

Withaferin A – A natural multifaceted therapeutic compound

Vinod

Senthil

2021

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COVID 19, which has lead to the death of millions of people, is still spreading worldwide. The development of any new drug after proper trial is much time consuming. This present global pandemic situation has lead the researchers around the world to run behind various existing antiviral and immunomodulatory natural compounds to overcome this contagious disease. Withania somnifera (ashwagandha) that is being used in Ayurvedic medicine for several ailments since several years is also said to pocess anti viral activity. Thus many of its metabolites are being studied individually for its efficacy against the dreadful disease. Withaferin A, a steroidal lactone from ashwagandha is one such prime metabolite which beyond acting as an antioxidant or antimicrobial agent, is also said to pocess anti-inflammatory to anti carcinogenic properties. Thus because of its wide spectrum of medicinal properties it has now become an attractive drug candidate for several preclinical studies. This increase in the demand for withaferin A has chanelled its way towards in vitro propagation of the plant Withania somnifera and trials on various strategies to increase the yield in terms of plant biomass as well as the withaferin content in the plants thus making it a better alternative to field grown plants. Thus this article reviews in depth on the important medicinal properties of withaferin A, its demand in Ayurveda industry and the in vitro strategies being carried out to overcome the demand.

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Section: Role Of Plant Tissue Culture In Wa Productionmentioning

confidence: 97%

Withaferin A – A natural multifaceted therapeutic compound

Vinod

Senthil

2021

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“…The report shows that Pb mainly precipitates in the cell wall of the root and only free Pb ions can be transported to other parts of the plant via xylem and phloem cells (Mathur & Chauhan, 2020). Therefore, in vitro liquid medium and shoot Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots cultures (Parameswari et al, 2017) were used for the Pb exposure study which facilitates free availability of Pb to the shoots. Hence the present study was focused to study the uptake of lead nitrate (Pb(NO 3 ) 2 ) by in vitro shoot cultures of W. somnifera and its influence on withaferin A (an important secondary metabolite native to W. somnifera) and the accumulation of lead in leaves.…”

Section: Introductionmentioning

confidence: 99%

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Natarajan,

Rangasamy,

Santhanu

et al. 2023

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Withania somnifera is a medicinal ayurvedic plant native to India. Lead (Pb) is a toxic heavy metal which has no biological function in plants. The aim of the present study is to investigate the influence of heavy metal Pb on W. somnifera at cellular level under controlled conditions. The role of Pb on shoots of W. somnifera was determined in terms of biomass, secondary metabolite production (High Performance Thin Layer Chromatography) and phytochemical quantification. Influence and accumulation of Pb at the cellular level were recognized using UV visible spectroscopy, field emission scanning electron microscope and x-ray diffraction analysis. The current result showed that the concentration up to 2.4 mM Pb for 7 days of exposure showed higher biomass and withaferin A yield compared to control shoots. On the other hand, other secondary metabolites such as flavonoids, phytosterols and phenols levels were reduced compared to control shoots. In the morphological study, Pb concentration in the analysed sample was determined as 2%. The probability of the nanoparticle nature of the bioaccumulated lead also verified using spectroscopy and x-ray diffraction analysis. To date, we are the first to report on the influence of Pb on in vitro shoot cultures of W. somnifera.

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A STUDY ON THE INFLUENCE OF PLANT GROWTH REGULATOR ON SHOOT MULTIPLICATION AND EVALUATION OF MAJOR WITHANOLIDES IN WITHANIA somnifera

Abstract: shoot that was analyzed using HPTLC. In vitro adventitious shoots in bioreactors could be used as a quick and efficient process of generating multiples that would offer unique opportunities for producing shoot based pharmaceutical products without having to depend on field cultivation.

Cited by 2 publications

References 18 publications

Withaferin A – A natural multifaceted therapeutic compound

Withaferin A – A natural multifaceted therapeutic compound

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

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