S ingle photon emission computed tomography (SPECT) is a major non-invasive diagnostic tool in nuclear medicine departments today. Collimated cameras, originally proposed by Hal Anger, have mostly been used for routine clinical applications. [1] As a result of mechanical collimation, system's geometrical properties limit and fix detection efficiency and energy acceptance. In conventional nuclear medicine applications, gamma rays emitted from various radiopharmaceuticals, especially 99m Technetium (99m Tc), are detected by systems called gamma cameras. Presently used gamma camera systems have Introduction: Quality control testing of a SPECT gamma camera is crucial in assessing the suitability of the camera for use in nuclear medicine department. The aim of the present study was to investigate the effect of gamma camera acquisition parameters on image quality. Methods: Camera scanning was carried out using a doubleheaded gamma camera on a total of 48 patients (29 female and 19 male, mean age: 47.4±11.1) referred to our department for myocardial perfusion scintigraphy and thyroid scintigraphy. Then, camera acquisition parameters were changed (for myocardial perfusion scintigraphy from 64x64 to 128x128 matrix change and for thyroid scintigraphy from 5 cm to 10 cm distance change), and scanning was repeated and images were analyzed. Results: Left ventricle ejection fraction (EF) value in 64x64 matrix was calculated to be 62.7±8.8%. EF value of 48.9±10.3% was obtained in 128x128 matrix for the same patients. 99mTc-pertechnetate uptake percentage was 3.8±2.3% in measurements carried out at a distance of 10 cm. On the other hand, a higher uptake percentage of 6.2±3.6% was found for the same patients measured at a distance of 5 cm. Discussion and Conclusion: In order to obtain proper imaging in SPECT gamma camera system, correct acquisition parameters should be used along with quality control tests for intrinsic floodfield uniformity and relative sensitivity.