A Study on the Effects of Continuous Mechanical Irritation to the Periodontal Tissues of Germfree and Conventional Rats

Irokawa, Toshinori; E, Ishida; Fukuoka, Yoshiaki; Maita, Eikichi; Yanagawa, Seiro

doi:10.2329/perio.19.333

Cited by 6 publications

(7 citation statements)

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“…Placement of an elastic ring around the cervix of M 1 induced an acute inflammatory response in the periodontal tissues as described in our previous study, 12,15 causing severe vertical and horizontal alveolar bone resorption between M 1 and M 2 , which resulted in a marked decrease in BMD. Using this experimental model, we investigated whether topical administration of a bisphosphonate clodronate could be effective in preventing alveolar bone loss.…”

Section: Discussionsupporting

confidence: 56%

“…On day 0, all of the rats were anesthetized with sodium pentobarbital (65 mg/kg, i.p. ), and a standardized elastic ring (diameter: 2.0 mm, thickness: 1.0 mm, width: 0.5 mm) was placed around the cervix of the maxillary first molars on both the right and left sides according to the method described by Shoji 12 et al and Irokawa et al 15 The topical administration of clodronate was started on the day the elastic ring was placed (day 0). Fifty µl of clodronate solution at a concentration of either 0 (0.9% NaCl), 20, 40, or 60 mM (CL-0, CL-20, CL-40, and CL-60 groups, respectively) was injected into the subperiosteum palatal area between either the left or right maxillary first and second molars on either side every other day for 7 days (days 0, 2, 4, and 6) under light pentobarbital anesthesia.…”

Section: Animal Experimentsmentioning

confidence: 99%

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Effects of Topical Administration of Clodronate on Alveolar Bone Resorption in Rats With Experimental Periodontitis

Mitsuta

Horiuchi

Satoh

2002

Journal of Periodontology

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Section: Discussionsupporting

confidence: 56%

Section: Animal Experimentsmentioning

confidence: 99%

Effects of Topical Administration of Clodronate on Alveolar Bone Resorption in Rats With Experimental Periodontitis

Mitsuta

Horiuchi

Satoh

2002

Journal of Periodontology

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“…Experimental research on food impaction has been performed with use of various animal species including rat, dog, monkey, et al, in which a variety of impaction methods were employed 27,29,[31][32][33][34][35][36][37][38][39] . Groove or pit formation at the contact surface of tooth, the combination of such deletion of tooth and mechanical dissocation, the insertion of various materials (gum, silk string, cotton string, gutter percha point) into the col were used.…”

Section: Discussionmentioning

confidence: 99%

Disturbance of Periodontal Tissue Caused by Mechanical Compression into the Gingival Col of Streptozotocin-induced Diabetic Rats

Tokunaga

Enoki

Okamura

et al. 2014

J. Hard Tissue Biology.

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FThe purpose of this study is to clarify the destructive processes of periodontal tissue, especially of alveolar bone, induced by food impaction. Streptozotocin-induced diabetic rat was used, gutter percha point (GP) was inserted into interdental gingival col space between upper M1 and M2, and the change of body weight, histopathological findings and 3D SEM structure of alveolar bone were compared between diabetic (Group DM) and control (Group N) animals (Experiment 1). Large amounts of bacterial deposits and sequester were observed at the alveolar crest, and subsequently experiment 2 was carried out to explore the relationship between bacterial infection and sequester formation. The combination of mechanical compression and daily twice cleaning with 3% oxydol and Periocline were carried out. As a result of experiment 1, ulceration with partial exposure of alveolar crest, inflammatory infiltrates and hyaline degeneration occurred in the col, and bone resorption was scarcely observed at 1d in Group N. Bone resorption of alveolar bone progressed at 3d and 5d, decreasing the height of alveolar crest and the width of alveolar bone. Reepithelization of ulcer surface was observed at 7d and 14d, and concurrently bone resorption regressed and new bone formation suggested reparative changes. SEM observation confirmed these changes of alveolar bone. On the contrary, massive bacterial deposits were observed, and bone resorption was scanty in the upper region and slight in the middle to lower region of alveolar bone at 1 day of Group DM. Massive bacterial deposits with partial exposure of alveolar bone were observed at 3d and 5d, and sequester was isolated owing to intense undermining bone resorption. Regeneration of epithelium was seen beneath the sequester at 14d, showing the phenomenon of foreign body exclusion. The light microscopic changes of Group DM were consistent with SEM findings. The total number of osteoclasts was fewer at 1d and larger at 3d and 5d in Group DM. Osteoclasts at the upper region of alveolar bone increased from 1d to 5d in Group N, although the number of osteoclasts did not show a significant change in Group DM. At the middle to lower portion of alveolar bone, the number of osteoclast was significantly fewer at 1d, larger at 3d and 5d in Group DM. In experiment 2, the col with ulceration and necrosis did not show bacterial deposits or sequester, suggesting the intimate relation between bacterial infection and sequestration. The present study suggests that food impaction and subsequent mechanical compression to the col could induce various damages of periodontal tissue, including sequester formation, and elaborate plaque control at the initial stage of food impaction could prevent sequester formation and protect the height of alveolar bone.

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“…On day 25, each group was divided into two subgroups (lactating or non‐lactating). On day 32, an elastic ring was placed around the neck of the right mandibular first molar to induce inflammatory periodontitis according to the method described by Irokawa et al 12 The left side of the mandible was not treated (used as a control). On day 46, all of the animals were sacrificed, and their mandibulae were dissected.…”

Section: Methodsmentioning

confidence: 99%

Effects of Lactation on Alveolar Bone Loss in Experimental Periodontitis

Shoji

Ohtsuka-Isoya

Shimauchi

et al. 2007

Journal of Periodontology

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A Study on the Effects of Continuous Mechanical Irritation to the Periodontal Tissues of Germfree and Conventional Rats

Cited by 6 publications

References 0 publications

Effects of Topical Administration of Clodronate on Alveolar Bone Resorption in Rats With Experimental Periodontitis

Effects of Topical Administration of Clodronate on Alveolar Bone Resorption in Rats With Experimental Periodontitis

Disturbance of Periodontal Tissue Caused by Mechanical Compression into the Gingival Col of Streptozotocin-induced Diabetic Rats

Effects of Lactation on Alveolar Bone Loss in Experimental Periodontitis

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