A Study on the Biological Control of Fusarium oxysporum Using Trichoderma spp., on Soil and Rockwool Substrates in Controlled Environment

Abstract

Medicinal plant cultivation in Controlled Environment (CE) is increasing in the context of the new findings concerning the abiotic stress factors manipulation that leads to a significant increment in bioactive substances. Pathogen control is a vital part of the cultivation system, therefore the study was focused on biological methods of controlling a frequently occurring disease, by inoculating the sterile substrates specific in hydroponics, with the beneficial organism. Hypericum perforatum seedlings were inoculated with Fusarium oxysporum and with Trichoderma spp. The results showed that the method of inoculation had a significant effect on the success of the biological control. The plants treated with Trichoderma spp. had a two-fold increase in foliar biomass and better development of roots than the plants inoculated with the pathogen. Morphologically there were no significant differences, with some notable exceptions. The health status of the seedlings inoculated with the pathogen showed signs of the disease, even in the presence of Trichoderma spp. Finally, determinations of Volatile Organic Compounds (VOCs) at the root level showed that the biotic stress was considerable higher in the rockwool substrate that increased the secondary metabolism giving new perspectives in the cultivation of medicinal plants in CEA.

Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita

“…In recent years, Trichoderma spp. has been used as biocontrol agents to control different plant pathogens [20,21]. Specifically, previous studies have reported that T. viride MTCC No.…”

Screening of Different Physalis Genotypes as Potential Rootstocks or Parents Against Vascular Wilt Using Physiological Markers

“…The alterations caused by vascular wilt on the leaf gas exchange properties and wf had a negative effect on total biomass accumulation (TDW) in most of the evaluated genotypes, except for P. floridana plants. Giurgiu et al (2018) and Chávez-Arias et al (2019) also recorded a reduction in biomass accumulation with periods of FO inoculation greater than 15 days in St. John's wort and cape gooseberry plants, respectively, with a reduction of water and nutrient uptake and an indirect decrease of the synthesis of photosynthetic pigments (Pantelides et al, 2013).…”

Utilização De Fungos Benéficos Na Formação De Mudas De Goiabeira Em Solos Infestados Com Nematoides

“…Trichoderma spp. foi inoculado em plantas de Hypericum perforatum (erva-de-sãojoão), e ocorreu o melhor desenvolvimento das raízes, contudo na temperatura entre 21 °C e 23 °C (GIURGIU et al, 2018).…”