A study on the antifungal susceptibility pattern of dermatophytes isolated in a tertiary care hospital

Sabtharishi, Vanathi; Katragadda, Radhika; Ravinder, Thyagarajan

doi:10.21746/ijbio.2017.05.003

Cited by 6 publications

(6 citation statements)

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“…Griseofulvin and Fluconazole had the poorest antifungal activity. This result was comparable to the study by Sharma et al,Khatri et al, and Budhiraja et al in India 26,28,29. According to the survey by Alim et al, Rahim et al, and Sabtharishi et al, Griseofulvin and Fluconazole had the poorest antifungal activity.…”

supporting

confidence: 84%

Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region

Khan¹,

Haque²,

Alam³

et al. 2022

TAJ: J of Teachers Assoc

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Background: Dermatophytosis is considered one of the most common public health problems in the world and is the most commonly diagnosed skin disease in Bangladesh. The prevalence and types of dermatophyte infections vary with climate conditions, age, lifestyle, and population migration patterns. Depending upon the anatomical site of the lesion, dermatophytes are classified into different varieties. Objective: To isolate dermatophytes and their antifungal susceptibility pattern by agar-based disc diffusion method in the Rajshahi region. Materials and Methods: Clinical samples (e.g., skin scrapings, nail clipping, and hair plucking) were collected under aseptic precautions. The identification of dermatophytes was performed through a microscopic examination using 10% KOH mount, mycological culture, and species identification by lactophenol cotton blue mount from culture. In addition, all dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-based disc diffusion method. Results: Out of 171 samples, Trichophyton rubrum was the predominant dermatophyte species with 76(71.7%), followed by T.mentagrophyte were 15(14.2%), E. floccosum were 12(11.3%), and M. canis were 03(2.8%). Voriconazole and Itraconazole were more effective drugs. Griseofulvin was the least effective drug, followed by Fluconazole. Conclusion: Despite several treatment options being available for cutaneous fungal infections, Due to the increasing trend of antifungal drug resistance among dermatophytes, treatment should be based on antifungal sensitivity testing. The disc diffusion method is a simple and valuable method for the evaluation of antifungal susceptibility of dermatophytes. TAJ 2022; 35: No-1: 99-106

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supporting

confidence: 84%

Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region

Khan¹,

Haque²,

Alam³

et al. 2022

TAJ: J of Teachers Assoc

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“…The E-test results correlate well with those of the disk diffusion method [14] [15]. Unlike disk diffusion, E-test MICs are unaffected by drug properties such as molecular weight, aqueous solubility as well as diffusion characteristics or by varying growth rates of different fungi [8]. In this study, we will adopt the agar based diffusion (ABDD) method and the E-test method to assess the occurrence of resistance in Trichophyton species against Fluconazole, Itraconazole, Terbinafine and Griseofulvin.…”

Section: Introductionmentioning

confidence: 57%

“…Hence, there arises the need for the correct, efficient, and rapid laboratory diagnosis of dermatophytes [8]. Another important point to consider is that resistance to antifungals has started appearing in dermatophytes [9].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Susceptibility Testing of Dermatophytes by ABDD and E-Test, a Comparative Study

Namidi¹,

Ananthnaraja²,

Satyasai³

2021

OJMM

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Aim: The objective of this study was to isolate, identify, and explore the in-vitro antifungal susceptibility pattern of dermatophytes isolated from clinically suspected cases of dermatophytosis (tinea infections) attending the Dermatology Department at J.S.S Hospital. Methods: This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under aseptic precautions. The identification of dermatophytes was performed through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-based disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. Results: A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tinea faciei. The dermatophytes isolated were Trichophyton rubrum 11 (35%), Trichophyton mentagrophyte 8 (25%), Trichophyton tonsurans 5 (16%), Microsporum gypseum 3 (10%), Trichophyton verrucosum 2 (6%), Trichophyton violaceum 1 (3%) and Microsporum audouinii 1 (3%). Out of 31 dermatophytes 17 were sensitive to all four antifungal agents within the range of FLC (2 -6 mcg/ml), ITR (0.125 -2), TER (0.125 -2), and GRI (0.125 -2), 5 isolates were resistant in which 2 were resistant to FLC (64.256 mcg/ml), 2 isolates were resistant to TER 2 (32.38 mcg/ml), 1 isolate was resistant to both GRI and TER (16.32 mcg/ml) and 9 isolates were within the intermediate range. Conclusion: Every patient with a tinea infection should be properly studied for a mycological examination and should be treated accordingly. Dermatophyte test medium is more useful as an identification medium in the How to cite this paper: Namidi, M.H.,

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“…Dermatophytosis accounts for more than 50% − 75% of fungal infection and is easily disseminated within a particular population [35]. Most dermatophyte infections are clinically diagnosed and are often misdiagnosed [36].…”

Section: Discussionmentioning

confidence: 99%

Antifungal Susceptibility of Pathogenic Moulds isolated from patients at Tertiary Hospital in South western Uganda

Bazira,

Nalumaga,

Tuhamize

et al. 2023

Preprint

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Fungal infections are common in tropical regions causing diverse human infections. Their isolation and sensitivity testing plays a major key role in the clinical management. Samples from eye clinic, skin scrapings and nail clippings were obtained and stained with potassium hydroxide, observed at 40x objective lens for hyphae identification. They were inoculated onto potato dextrose agar slant and incubated for 14 days for mould isolation. Lactophenol cotton blue stain was used for macroscopic identification of Moulds. 3 antifungal powder agents were dissolved in RPMI-1640 solution for the MIC of the isolated Moulds. Of 40 Moulds isolated 30(75%) were skin scrapings, 5(12.5%) were corneal scrapings and 5(12.5) were nail scrapings. Of these isolates; 10 Aspergillus niger, 6 Penicillium, 5 Trichophyton mentagrophytes, 4 Curvularia, 5 Rhizopus, 4 Fusarium, 1 Bipolaris and fusarium, 1 Lasidiopolidia, 1 Collectrium, 2 Aspergillus fumigatus, 1 Aspergillus flavus. For MIC, Itraconazole had 39 sensitive and 1 resistant, Fluconazole had 35 sensitive and 5 resistant while Amphotericin B had 32 sensitive and 8 resistant isolates. Amphotericin B increased resistance is attributed to readily available polyene creams in the drug shops across Uganda.

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A study on the antifungal susceptibility pattern of dermatophytes isolated in a tertiary care hospital

Cited by 6 publications

References 0 publications

Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region

Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region

Antifungal Susceptibility Testing of Dermatophytes by ABDD and E-Test, a Comparative Study

Antifungal Susceptibility of Pathogenic Moulds isolated from patients at Tertiary Hospital in South western Uganda

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