A study on the ability of quaternary ammonium groups attached to a polyurethane foam wound dressing to inhibit bacterial attachment and biofilm formation

Tran, Phat L.; Hamood, Abdul N.; Souza, Anselm de; Schultz, Gregory S.; Liesenfeld, Bernd; Mehta, Dilip; Reid, Ted W.

doi:10.1111/wrr.12244

Cited by 49 publications

(23 citation statements)

References 37 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the biofilms were examined by CLSM using a Nikon A1+/AIR+ Confocal Microscope (Nikon Inc., Melville, NY). NIS‐Elements 2.2 software (Nikon) was used to generate three‐dimensional biofilm images as described previously . All experiments were conducted in at least triplicate.…”

Section: Methodsmentioning

confidence: 99%

Preparation of chitin‐CdTe quantum dots films and antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa

Wansapura

Dassanayake

Hamood³

et al. 2017

J of Applied Polymer Sci

View full text Add to dashboard Cite

Novel chitin–cadmium‐tellurium quantum dot (Chitin‐CdTeQD) hybrid films combining chitin and CdTe quantum dots (CdTeQDs) were prepared via a facile aqueous synthesis route at room temperature. Films were characterized by high‐resolution field emission scanning electron microscopy (HR‐FESEM) and energy dispersive X‐ray (EDX) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and X‐ray diffraction analysis (XRD). Antibacterial activity was studied on both Gram‐positive (Staphylococcus aureus) and Gram‐negative (Pseudomonas aeruginosa) bacteria. Antibacterial properties were investigated with agar diffusion testing assay and with confocal laser scanning microscopic image analysis. Chitin–CdTeQD films exhibited an excellent antibacterial activity against both Gram‐positive and Gram‐negative bacteria. Chitin–CdTeQD films might be a desirable antibacterial material for wide range of biomedical applications including wound dressing, burn treatment, drug delivery systems, packaging, ophthalmology, and implants. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44904.

show abstract

Section: Methodsmentioning

confidence: 99%

Preparation of chitin‐CdTe quantum dots films and antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa

Wansapura

Dassanayake

Hamood³

et al. 2017

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…Attempts have also been made to incorporate QA groups into wound dressing materials. Attachment of QA groups to a polyurethane foam wound dressing [220] resulted in inhibition of biofilm development of three major wound pathogens (S. aureus, P. aeruginosa and Acinetobacter baumannii) both within the wound and in the wound dressing. Wound healing requires a moist and warm environment, and dressing materials with good air permeability and moisture absorption are desirable.…”

Section: Sutures and Wound Dressingsmentioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

483

317

View full text Add to dashboard Cite

a b s t r a c tMicrobial infections affect humans worldwide. Many quaternary ammonium compounds have been synthesized that are not only antibacterial, but also possess antifungal, antiviral and anti-matrix metalloproteinase capabilities. Incorporation of quaternary ammonium moieties into polymers represents one of the most promising strategies for preparation of antimicrobial biomaterials. Various polymerization techniques have been employed to prepare antimicrobial surfaces with quaternary ammonium functionalities; in particular, syntheses involving controlled radical polymerization techniques enable precise control over macromolecular structure, order and functionality. Although recent publications report exciting advances in the biomedical field, some of these technological developments have also been accompanied by potential toxicological and antimicrobial resistance challenges. Recent evidenced-based data on the biomedical applications of antimicrobial quaternary ammonium-containing biomaterials that are based on randomized human clinical trials, the golden standard in contemporary medicinal science, are included in the present review. This should help increase visibility, stimulate debates and spur conversations within a wider scientific community on the implications and plausibility for future developments of quaternary ammonium-based antimicrobial biomaterials.Published by Elsevier B.V.

show abstract

“…Previous studies on the antimicrobial properties of a non‐leaching urethane wound‐dressing material that has long chain polymers with high densities of quaternary amines (pDADMAC) attached to its surface showed that they were effective in controlling bacterial biofilms when studied in vitro . The present study was carried out to see whether the same wound‐dressing material can play an active role in the treatment process of a wound in vivo using a mouse wound model.…”

Section: Introductionmentioning

confidence: 95%

The ability of quaternary ammonium groups attached to a urethane bandage to inhibit bacterial attachment and biofilm formation in a mouse wound model

Tran

Hamood

Souza³

et al. 2015

International Wound Journal

Self Cite

View full text Add to dashboard Cite

For proper wound healing, control of bacteria or bacterial infections is of major importance. While caring for a wound, dressing material plays a key role as bacteria can live in the bandage and keep re-infecting the wound. They do this by forming biofilms in the bandage, which slough off planktonic bacteria and overwhelm the host defense. It is thus necessary to develop a wound dressing that will inhibit bacterial growth. This study examines the effectiveness of a polyurethane foam wound dressing bound with polydiallyl-dimethylammonium chloride (pDADMAC) to inhibit the growth of bacteria in a wound on the back of a mouse. This technology does not allow pDADMAC to leach away from the dressing into the wound, thereby preventing cytotoxic effects. Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii were chosen for the study to infect the wounds. S. aureus and P. aeruginosa are important pathogens in wound infections, while A. baumannii was selected because of its ability to acquire or upregulate antibiotic drug resistance determinants. In addition, two different isolates of methicillin-resistant S. aureus (MRSA) were tested. All the bacteria were measured in the wound dressing and in the wound tissue under the dressing. Using colony-forming unit (CFU) assays, over six logs of inhibition (100%) were found for all the bacterial strains using pDADMAC-treated wound dressing when compared with control-untreated dressing. The CFU assay results obtained with the tissues were significant as there were 4-5 logs of reduction (100%) of the test organism in the tissue of the pDADMAC-covered wound versus that of the control dressing-covered wound. As the pDADMAC cannot leave the dressing (like other antimicrobials), this would imply that the dressing acts as a reservoir for free bacteria from a biofilm and plays a significant role in the development of a wound infection.

show abstract

A study on the ability of quaternary ammonium groups attached to a polyurethane foam wound dressing to inhibit bacterial attachment and biofilm formation

Cited by 49 publications

References 37 publications

Preparation of chitin‐CdTe quantum dots films and antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa

Preparation of chitin‐CdTe quantum dots films and antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

The ability of quaternary ammonium groups attached to a urethane bandage to inhibit bacterial attachment and biofilm formation in a mouse wound model

Contact Info

Product

Resources

About