A Study on Steroid Hormone Receptors in the Rabbit Oviduct and Uterus During the First Few Days after Coitus and During Egg Transport

El-Banna, Amin A.; Sacher, Bernhard

doi:10.1095/biolreprod17.1.1

Cited by 18 publications

(5 citation statements)

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“…terms of uterine content, of the specific PR mRNA and related lower size transcripts were noted with maximal concentrations on days 2-3 after mating. These findings confirm and extend previous studies of the rabbit endometrial PR in early pregnancy using binding techniques (El-Banna and Sacher, 1977;Young et al, 1981).…”

Section: Discussionsupporting

confidence: 91%

Endometrial expression of progesterone receptor and uteroglobin genes during early pregnancy in the rabbit

Gutiérrez-Sagal¹,

Pérez‐Palacios²,

Langley³

et al. 1993

Molecular Reproduction Devel

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The progesterone receptor (PR) plays a pivotal role in the maturation process of the secretory endometrium, implantation and maintenance of pregnancy in rabbits. To determine the dynamics of PR gene expression and its physiological significance, the endometrial expression of PR and PR mRNA were evaluated and compared with the expression of the progesterone-regulated uteroglobin (UG) gene during 0-5 days post-coitus in rabbits. The results of immunoblot experiments indicated the presence of PR in endometrial cell extracts from days 1-4 of pregnancy with maximum PR immunostaining on day 2, followed by a marked diminution until its complete disappearance on day 5. When endometrial PR mRNA content was assessed by Northern blots, the results were similar to those of PR immunostaining, with maximal concentrations on the second day after mating. However, PR mRNA levels were still high on day 3, despite the concomitant decrease in immunostainable PR. Endometrial UG gene expression, on the other hand, exhibited a different time sequence. Thus, the UG content in uterine flushings progressively increased from day 3 after mating, reaching maximal levels on the fifth day. The endometrial UG mRNA content presented a similar profile, as its maximum concentration occurred on days 4-5. The overall results indicate that endometrial PR is down-regulated at both the mRNA and protein levels, possibly by endogenous progesterone during early pregnancy. The striking observation that maximal expression of endometrial UG gene products occurred when PR and its mRNA are no longer detectable suggests an important role for this progesterone-binding uterine protein during the preimplantation period.

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Section: Discussionsupporting

confidence: 91%

Endometrial expression of progesterone receptor and uteroglobin genes during early pregnancy in the rabbit

Gutiérrez-Sagal¹,

Pérez‐Palacios²,

Langley³

et al. 1993

Molecular Reproduction Devel

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“…This adverse effect of ZEA on embryo transport in the oviduct is most likely attributed to its estrogenicity. It has been documented that estrogen and estrogenic chemicals can delay oviductal oocyte or embryo transport in cows (Wijayagunawardane et al, 1998), rabbits (El-Banna andSacher, 1977), mice, guinea pigs, and hamsters (Greenwald, 1967;Xiao et al, 2011), but accelerate oviductal oocyte or embryo transport in rats (Akira et al, 1993;Greenwald, 1967;Ortiz et al, 1979). The molecular mechanism of estrogen and estrogenic compounds on oviductal transport is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Postweaning Exposure to Dietary Zearalenone, a Mycotoxin, Promotes Premature Onset of Puberty and Disrupts Early Pregnancy Events in Female Mice

Zhao¹,

Li²,

Xiao³

et al. 2013

Toxicological Sciences

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Zearalenone (ZEA) is a mycotoxin commonly found in contaminated livestock feed and human food with levels in the range of ppb and low ppm. It was hypothesized that ZEA, an endocrine disruptor, could affect puberty and early pregnancy. To test this hypothesis, newly weaned (3 weeks old) C57BL/6J female mice were exposed to 0, 0.002, 4, 10, and 40 ppm ZEA and 0.05 ppm diethylstilbestrol (positive control) in phytoestrogen-free AIN-93G diet. Females exposed to 10 and 40 ppm ZEA diets showed earlier onset of vaginal opening. Those treated with 40 ppm ZEA diet also had earlier first copulation plug and irregular estrous cyclicity. At 8 weeks old, all females were mated with untreated stud males on AIN-93G diet during mating. Treatment resumed upon identification of a vaginal plug on gestation day 0.5 (D0.5). Embryo implantation was assessed on D4.5. Exposure to 40 ppm ZEA diet resulted in reduced percentage of plugged mice with implantation sites, distended uterine appearance, and retained expression of progesterone receptor in D4.5 uterine epithelium. To determine the exposure timing and mechanisms of disrupted embryo implantation, four groups of females were fed with 0 or 40 ppm ZEA diets during premating (weaning to mating) and postmating (D0.5-D4.5), respectively. Premating exposure to 40 ppm ZEA diet reduced fertilization rate, whereas postmating exposure to 40 ppm ZEA diet delayed embryo transport and preimplantation embryo development, which subsequently affected embryo implantation. These data demonstrate that postweaning exposure to dietary ZEA can promote premature onset of puberty and disrupt early pregnancy events.

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“…Pregnancy is associated with high circulating levels of estrogen and progesterone, and the gross changes in microvasculature reported in this study are, in all probability, the result of raised levels of these hormones. Around the time of ovulation known varia tions in rabbit ovarian venous estradiol and proges terone [Hilliard and Eaton, 1971] and tubal estradiol and progesterone receptor binding [El Banna and Sacher, 1977] may be associated with changes in tubal venous distension similar to those observed in preg nancy. The relationship between ovarian or placental hormones and tubal microvasculature implied by this study, and by investigation of tubal MVA after oophorectomy [Verco et al, in press], is of particular interest when considering the proposal that variations of isthmic subserosal venous pressure or tone may be relevant in tubal transport [Verco et al, 1983a].…”

Section: Discussionmentioning

confidence: 67%

Microvascular Architecture of the Pregnant Rabbit Oviduct

Verco¹,

Gannon²,

Jones³

1984

Cells Tissues Organs

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Methyl-methacrylate vascular corrosion casts of the oviducts were prepared in 7 rabbits which were 2–3 weeks pregnant. Scanning electron microscopy of the acrylic casts revealed little change in tubal microvascular connections when compared with control oviducts. Venous distension in the isthmic subserosal venous plexus, ampullary subserosal vasculature and in the fimbrial core was substantially greater than that observed in controls. These changes are interpreted as indicating a sensitivity of tubal microvasculature to the increased levels of circulating placental hormones in pregnancy. The implications of this interpretation in the role of tubal microvasculature at the time of ovulation are discussed.

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A Study on Steroid Hormone Receptors in the Rabbit Oviduct and Uterus During the First Few Days after Coitus and During Egg Transport

Abstract: The variations in estrogen,

Cited by 18 publications

References 0 publications

Endometrial expression of progesterone receptor and uteroglobin genes during early pregnancy in the rabbit

Endometrial expression of progesterone receptor and uteroglobin genes during early pregnancy in the rabbit

Postweaning Exposure to Dietary Zearalenone, a Mycotoxin, Promotes Premature Onset of Puberty and Disrupts Early Pregnancy Events in Female Mice

Microvascular Architecture of the Pregnant Rabbit Oviduct

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