The purpose of this study is to present the base for activation policy for cost estimation guidelines of e-Learning content development. To achieve this purpose, this study investigates the effectiveness of cost estimation guidelines of e-Learning content development through a survey about executives and employees in the e-Learning industry and analyses them statistically. According to an analysis, companies(which abandoned order because the amount of money (cost) of e-Learning contents ordering is low and suffered a loss after acquiring e-Learning contents project order) have a relatively negative awareness about the effectiveness of cost estimation guidelines of e-Learning contents. On the other hand, contents focused companies have a relatively positive awareness about the effectiveness of cost estimation guidelines of e-Learning contents. In conclusion, this study suggests that government should recommend and enact·announce the use of cost estimation criteria(guidelines) of e-Learning content development and provide the institutional tools soon as possible.