This study is aimed to analyze the mediating effect of knowledge sharing on relationships between the organizational culture and innovative behavior of nursing care providers, and providing implications for improving the quality of services by virtue of innovative behaviors of those providers.To achieve the research objectives, the study conducted a questionnaire survey of care workers for nursing homes located in Busan and Daegu from August 10 to August 31, 2020. Among pieces of the questionnaire form filled out by the participants that were collected through the survey, the study analyzed 335 ones in total using SPSS 21.0 and AMOS 23.0 Programs. The findings of this research can be briefly described as follows. First, care workers serving at nursing homes showed significant differences in relation-oriented culture, innovation-oriented culture, knowledge sharing and innovative behavior level by their demographic characteristics. Second, it was found that organizational culture of those workers, except for innovation-oriented culture, had a significant effect on their knowledge sharing. Third, it was also found that the stronger the organizational culture was, the higher the innovative behavior level was. Fourth, it was discovered that knowledge sharing between care workers had a significant influence on their innovation behavior. And fifth, the knowledge sharing was found having a partially mediating impact on relationships between care workers' organizational culture and innovative behavior. When these findings are taken into consideration, this study is intended to provide important implications for higher-quality services from care workers since it turned out that improvement in the organizational culture and knowledge sharing of professional care providers can contribute to strengthening their innovative behaviors.