A study on nucleolar silver staining in plant cells. The role of argyrophilic proteins in nucleolar physiology

Medina, F. Javier; Risueño, María Carmen; Sánchez-Pina, M. Amelia; Fernández-Gómez, M. E.

doi:10.1007/bf00327336

Cited by 74 publications

(39 citation statements)

References 28 publications

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“…Obviously, the significantly positive correlation between NOR stainability and the presence of a secondary constriction clearly supports the idea that the decondensed state of the NOR chromatin is a necessary prerequisite to its impregnation by silver (Medina et al, 1983). Accordingly, in T occidentalis as well as in E. quercinus chromosomes, there were no cases in which condensed secondary constrictions were Ag-stained.…”

Section: Discussionsupporting

confidence: 66%

“…On the other hand, although mitotic NOR's are known to be transcriptionally inactive and the inhibition of rRNA synthesis by cycloheximide (CHM) does not abolish silver staining (Medina et al, 1983), it could be considered that transcriptional activity is certainly necessary for the Agstainability, but this need not occur precisely at the same time as mitotic chromosome fixation.…”

Section: Discussionmentioning

confidence: 99%

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A study of the Ag-staining significance in mitotic NOR's

1988

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The biological significance of nucleoli and mitotic Ag-staining NOR's has been the cause of controversy for several years. Whereas several authors state or assume a direct correlation between transcriptional activity and Ag-staining, others deny this hypothesis or claim a correlation between Ag-staining and decondensation of the NOR chromatin. Our results show that a decondensed state of the NOR chromatin is necessary but not sufficient to permit silver impregnation, allowing us to conclude that transcriptional NOR activity in the previous interphase is additionally required for silver staining.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

A study of the Ag-staining significance in mitotic NOR's

1988

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“…To stain NORs with silver requires the NOR chromatin to be in a decondensed state (nucleolar constriction) (Clavaguera et al, 1983;Medina et al, 1983;Sánchez-Pina et al, 1984, Jiménez et al, 1988, and the ribosomal genes to have transcriptional activity during the preceding interphase (see Hubbel, 1985). Thus, size variations between NORs with homologous chromosomes in M. trichodon could be interpreted as differential transcriptional activity of the ribosomal genes.…”

Section: Cytogeneticmentioning

confidence: 99%

Karyological characterization of <i>Mugil trichodon</i> Poey, 1876 (Pisces: Mugilidae)

Nirchio¹,

Ron²,

Rosi³

2005

Sci. Mar.

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“…This hypothesis has been refuted by others (Raman & Sperling, 1981;Medina et a!., 1983), who supported the view that Ag staining requires only a decondensed state of the NOR chromatin (nucleolar constriction) to occur.…”

Section: Introductionmentioning

confidence: 97%

Interchromosomal, intercellular and interindividual variability of NORs studied with silver staining and in situ hybridization

et al. 1997

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We investigated the relationship between transcriptional activity and the quantity of ribosomal cistrons of the nucleolar organizing regions (NORs) in chromosomal pair 3 of the Spanish mole Talpa occidentalis. Transcriptional activity was estimated by the size of conventional silver-staining signals. The number of ribosomal cistrons was estimated by the size of the in situ hybridization signals obtained with a ribosomal DNA probe. A sample of cells was studied with each method in four different individuals, and the results obtained with the two techniques were compared. Furthermore, sequential analysis on the same cells was carried out to study the association of interstitial nucleolar constrictions with silver staining and in situ hybridization. Interchromosomal, intercellular and interindividual variability were found with both silver staining and in situ hybridization methods. Our results show that transcriptional activity of NORs does not depend exclusively on the number of ribosomal cistrons they have as a high percentage of cells had two NORs with abundant ribosomal cistrons, only one of which was active. Additional factors, probably responsible for the activation of transcription, may be involved in this variability.

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A study on nucleolar silver staining in plant cells. The role of argyrophilic proteins in nucleolar physiology

Cited by 74 publications

References 28 publications

A study of the Ag-staining significance in mitotic NOR's

A study of the Ag-staining significance in mitotic NOR's

Karyological characterization of <i>Mugil trichodon</i> Poey, 1876 (Pisces: Mugilidae)

Interchromosomal, intercellular and interindividual variability of NORs studied with silver staining and in situ hybridization

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