A study on morphological changes and histamine release induced by compound 4880 in rat peritoneal mast cells

Bloom, Gunnar D.; Hægermark, ÖSten

doi:10.1016/0014-4827(65)90241-7

Cited by 151 publications

(69 citation statements)

References 12 publications

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“…The granules which have degranulated are usually without perigranular membrane (15). There are also observations which support the fact that histamine release occurs even without degranulation (1)(2)(3).…”

Section: Methodsmentioning

confidence: 56%

Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Yamasaki

Sugiyama

1972

Jpn.J.Pharmacol

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It is generally recognized that the histamine release from mast cells induced by a number of histamine releasers including compound 48/80 or by antigen-antibody reaction is ac companied by extracellular discharge of granules (degranulation), though this is not always the case (1-3). Uvnäs and Thon (4, 5) proposed a hypothesis that the mechanism of histamine release from mast cells by compound 48/80 consists of two consecutive steps: the one being the process of extracellular discharge of granules, and the other, that occurs subsequently, a process of the release of histamine from extracellular granules. They consider that the first process is energy-requiring but the second one is a non-enzymatic simple ion exchange which occurs in the extracellular fluid phase between granular histamine and inorganic cations.

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Section: Methodsmentioning

confidence: 56%

Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Yamasaki

Sugiyama

1972

Jpn.J.Pharmacol

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“…Secretion of histamine is induced by noncytotoxic agents such as A 23187 (13), compound 48/80 (2,3,6,14,17,24,26), polymyxin B (4,7,8,17,18) and antibodies (1,19). Histamine is stored in the heparin-protein complex released by anion exchange between the histamine in the secretory granules and cations in the extracellular milieu (1).…”

mentioning

confidence: 99%

Reorganization of Membrane Cholesterol at anEarly Stage of Exocytosis (Degranulation) in Rat Mast Cells: Inferred from the Ditribution of Filipin-Cholesterol Comlexes

Sekiya

Banno

Nozawa

1981

Cell Struct. Funct.

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“…Many of the ultrastructural features of anaphylactictype degranulation in rat peritoneal mast cells have been described in detail (Bloom and Haegermark 1965;Singleton and Clark 1965;Bloom and Chakravarty 1970;Röhlich et al 1971;Röhlich and Csaba 1972;Lagunoff 1972Lagunoff ,1973Dvorak 1991). Therefore, in this study, we focused on the ultrastructural findings that preceded the formation of cytoplasmic degranulation channels and the exteriorization of granule contents.…”

Section: Ultrastructural Features Of Mast Cells During the First 10 Smentioning

confidence: 99%

“…The mast cells were diluted in HBSS to a final concentration of 1 ϫ 10 6 cells/ml and were maintained at 4C (for up to 60 min) until stimulation. Cells were stimulated with compound 48/80 (Sigma Chemical; St Louis, MO) (10 g/ml in HBSS, 20C) (Bloom and Haegermark 1965;Bloom and Chakravarty 1970;Röhlich et al 1971;Uvnäs 1974;Chandler and Heuser 1980;Login et al 1992;Takayama et al 1994). Aliquots (100 l) of mast cells in suspension were added to 200-l aliquots of HBSS containing compound 48/ 80 at room temperature (RT) in a polystyrene cuvette (0.1 cm 2 ϫ 4.5 cm; Fisher Scientific, Pittsburgh, PA) and incubated at RT for 1,5,6,7,8,9, or 10 sec.…”

Section: Preparation and Stimulation Of Peritoneal Mast Cell Suspensionsmentioning

confidence: 99%

“…This process is characterized morphologically by granule swelling followed by the relatively rapid fusion of the membranes surrounding individual cytoplasmic granules with each other and with the plasma membrane (Bloom and Haegermark 1965;Bloom and Chakravarty 1970;Röhlich et al 1971;Lagunoff 1972;Röhlich and Csaba 1972;Dvorak et al 1983;Galli et al 1984;Dvorak 1991). Although these features of the secretory response have been well described (Bloom and Chakravarty 1970;Röhlich et al 1971;Lagunoff 1973;Kagayama and Douglas 1974;Lawson et al 1977;Chandler and Heuser 1980;Kraeuter Kops, et al 1990), no studies have systematically analyzed vesicle events in the 0-10 sec time period preceding exocytosis.…”

mentioning

confidence: 99%

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Immunocytochemical Localization of Chymase to Cytoplasmic Vesicles After Rat Peritoneal Mast Cell Stimulation by Compound 48/80

Aoki

Yamakawa

et al. 1997

J Histochem Cytochem.

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SUMMARYThe subcellular events responsible for release of mediators by mast cells may help to clarify roles for mast cells in health and disease. In this study we show that the granule-associated protease chymase is also within cytoplasmic vesicles in appropriately stimulated rat peritoneal mast cells. Rat peritoneal mast cells were recovered before or 1-10 sec after exposure to the secretogogue compound 48/80 (10 g/ml) and then were examined by radioimmunoassay to quantify histamine release or were processed, using routine methods for postembedding immunoelectron microscopy, to identify the subcellular localization of chymase. In comparison to unstimulated cells, compound 48/80 stimulated cells in two independent experiments showed an increase (15%, 28%) in the surface area of the cell and a decrease (12%, 6%) in the surface area of the total granule compartment before degranulation channel formation. These global cellular changes occurred in a background of transient but significant ( p Ͻ 0.01) increases in the area and number of chymase-immunoreactive vesicles per m 2 cytoplasm. These changes were detectable at 5 or 7 sec after stimulation with compound 48/80 but returned to near prestimulation levels by 9 or 10 sec after addition of compound 48/80 (total cumulative histamine release was 28% by 8 sec and 47% by 14 sec). These observations suggest that vesicles participate in the early stages of regulated secretion of chymase from rat peritoneal mast cells.

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A study on morphological changes and histamine release induced by compound 4880 in rat peritoneal mast cells

Cited by 151 publications

References 12 publications

Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Reorganization of Membrane Cholesterol at anEarly Stage of Exocytosis (Degranulation) in Rat Mast Cells: Inferred from the Ditribution of Filipin-Cholesterol Comlexes

Immunocytochemical Localization of Chymase to Cytoplasmic Vesicles After Rat Peritoneal Mast Cell Stimulation by Compound 48/80

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