A study on microvascular density in breast carcinoma

Abstract

Background: Breast Cancer is the most frequent neoplasm causing death in women between 35-55 years of age. Of the Prognostic indicators existing for breast cancer, axillary lymph node status has been regarded as the most important one. Twenty to thirty percent of all lymph node negative patients will still develop a recurrence of the disease within 10 years of initial treatment. Therefore, a new prognostic marker that could identify patients at high risk of tumor recurrence more accurately than existing indicators would be of great value, one potential indicator is tumor-induced angiogenesis. Materials and Methods: This is a six months prospective (January 2010-June 2010) and 1 year retrospective (Jan2009-Dec2009) study which included thirty five breast cancer cases visiting the Surgical OPD. Angiogenesis was estimated by determining micro vessel counting after immune staining the paraffin embedded tissue sections using anti-CD34 antibody. Results: Age of the patients ranged from 25 to 80 years with a mean age of 45.48 years. Most of the cases were infiltrating ductal carcinoma comprising of 33 cases (94.28%). Three cases (9.10%) showed vascular invasion by the tumor. Majority showed (63.64%) vessel count of less than 200 per 10 high power fields. Conclusion: Micro vascular density positively correlated with size of the tumor, lymph nodes involved by the tumor and Nottingham prognostic index. In the future, Antibodies specific to proliferating endothelium, together with the development of automated image analysis, may improve the accuracy and value of measuring angiogenesis-induced microvessel density. DOI: http://dx.doi.org/10.3126/jpn.v4i7.10315 Journal of Pathology of Nepal (2014) Vol. 4, 570-575

Ezh2, endothelin-1, and cd34 as biomarkers of aggressive cervical squamous cell carcinoma: an immunohistochemical study

“…This observation was similar to the previous studies (12,25,26) that were done on colorectal carcinoma, breast cancer and oral squamous cell carcinoma respectively.…”

“…The same result was obtained by Rofstad et al who reported that increased neo-angiogenesis in tumor tissues illustrated the direct spread to lymph nodes in cervical cancer (24). Such a correlation was reported by another study on breast cancer (25). By contrast, another study found no correlation between MVD and clinicopathologic variables (28).…”

“…By contrast, another study found no correlation between MVD and clinicopathologic variables (28). Tumor heterogeneity, the type of the endothelial marker used, hot spot selection and counting, angiogenesis heterogeneity, methods of detection and observer variability could explain these discrepancies (25).…”

“…Measurement of MVD by immunostaining of endothelial marker as CD34 is used for angiogenesis evaluation. A significant association between intra-tumoral MVD and the prognosis of many tumors was published (25).…”

Significance Of Micro Vessel Density In Invasive Breast Carcinoma

“…[7] Pyakurel D et al, in his study concluded that MVD positively correlated with size of the tumour, lymph nodes with tumour grade and aggressiveness. [11] Teo et al showed that higher the microvessel densities in breast cancers, higher the risk of subsequent in situ cancer and invasive recurrence of previous in situ cancers with poorer response to treatment. [12] Majority of published reports have shown a significant correlation between the density of intratumoral micro vessels in invasive breast carcinoma and the incidence of metastasis and or survival.…”