A Study on Mercury-Resistant Bacteria Isolated from a Gold Mine in Pongkor Village, Bogor, Indonesia

Irawati, Wahyu; Patrícia,; Soraya, Yenny; Baskoro, Abyatar Hugo

doi:10.4308/hjb.19.4.197

Cited by 37 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genus Brevundimonas, which is significantly enriched in SL3 metagenome has been recovered by several workers from mercury-contaminated environments. Irawati et al (2012) isolated two highly Hg-resistant (MIC 575 ppm) species, Brevundimonas sp. HgP1 and Brevundimonas sp.…”

Section: Discussionmentioning

confidence: 99%

Mercury contamination imposes structural shift on the microbial community of an agricultural soil

Salam¹,

Shomope

Ummi

et al. 2019

Bull Natl Res Cent

View full text Add to dashboard Cite

Background: The purpose of this study is to use shotgun next-generation sequencing to unravel the microbial community structure of an agricultural soil, decipher the effects of mercury contamination on the structure of the microbial community and the soil physicochemistry and heavy metals content. Results: The soil physicochemistry after mercury contamination revealed a shift in soil pH from neutral (6.99 ± 0.001) to acidic (5.96 ± 0.25), a decline in moisture content to < 4 %, and a significant decrease in the concentrations of all the macronutrients and the total organic matter. Significant decrease in all the heavy metals detected in the agricultural soil was also observed in mercury inundated SL3 microcosm. Structural analysis of the metagenomes of SL1 (agricultural soil) and SL3 (mercury-contaminated agricultural soil) using Illumina shotgun next-generation sequencing revealed the loss due to mercury contamination of 54.75 % of the microbial community consisting of an archaeal domain, 11 phyla, 12 classes, 24 orders, 36 families, 59 genera, and 86 species. The dominant phylum, class, genus, and species in SL1 metagenome are Proteobacteria, Bacilli, Staphylococcus, and Sphingobacterium sp. 21; while in SL3 metagenome, Proteobacteria, Alphaproteobacteria, Singulisphaera, and Singulisphaera acidiphila were preponderant. Mercury contamination resulted in a massive upscale in the population of members of the phylum Planctomycetes and the genera Singulisphaera, Brevundimonas, Sanguibacter, Exiguobacterium, Desulfobacca, and Proteus in SL3 metagenome while it causes massive decline in the population of genera Staphylococcus and Brachybacterium. Conclusions: This study revealed that mercury contamination of the agricultural soil imposed selective pressure on the members of the microbial community, which negatively impact on their population, alter soil physicochemistry, and enriched sizable numbers of members of the community that are well adapted to mercury stress. It also reveals members of microbial community hitherto not reported to be important in mercury detoxification process.

show abstract

Section: Discussionmentioning

confidence: 99%

Mercury contamination imposes structural shift on the microbial community of an agricultural soil

Salam¹,

Shomope

Ummi

et al. 2019

Bull Natl Res Cent

View full text Add to dashboard Cite

show abstract

“…While some microbes do not have the mer operon, they could have other ways to resist Hg. These methods could include influx, accumulation, efflux, and metallothionein, a protein that protects the cell against the toxicity of Hg (Irawati et al, 2012). It is important to know the many diverse ways that microorganisms resist toxic metals.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Characterizing and Evaluating the Diverse Microbial Communities and Their Mercury Resistance Potential from Hot Spring Sites Representing Gradients in Temperature and pH in Yellowstone National Park

Rassadkina¹,

Roth²,

Barkay³

2020

ArestyRURJ

View full text Add to dashboard Cite

Yellowstone National Park is home to many different hot springs, lakes, geysers, pools, and basins that range in pH, chemical composition, and temperature. These different environmental variations provide a broad range of conditions that select and grow diverse communities of microorganisms. In this study, we collected samples from geochemically diverse lakes and springs to characterize the microbial communities present through 16S rRNA metagenomic analysis. This information was then used to observe how various microorganisms survive in high mercury environments. The results show the presence of microorganisms that have been studied in previous literature. The results also depict gradients of microorganisms including thermophilic bacteria and archaea that exist in these extreme environments. In addition, beta diversity analyses of the sequence data showed site clustering based primarily on temperature instead of pH or sample site, suggesting that while pH, temperature, and sample site were all shown to be significant, temperature is the strongest factor driving microorganism community development. While it is important to characterize the microorganism community present, it is also important to understand how this community functions as a result of its selection. Along with looking at community composition, genomic material was tested to see if it contained mercury methylating (hgcA) or mercury reducing (merA) genes. Out of 22 samples, three of them were observed to have merA genes, while no samples had hgcA genes. These results indicate that microorganisms in Mustard and Nymph Springs may use mercury reduction. Understanding how microorganisms survive in environments with high concentrations of toxic pollutants is crucial because it can be used as a model to better understand mechanisms of resistance and the biogeochemical cycle, as well as for bioremediation and other solutions to anthropogenic problems.

show abstract

“…Remediasi lingkungan tercemar merkuri merupakan langkah yang amat penting dilakukan, karena merkuri tidak dapat terdegradasi di alam. Beberapa langkah penanggulangan lahan tercemar merkuri dapat ditempuh melalui metode eskavasi, pembuangan, elektro-remediasi, pencucian tanah, dan desorpsi termal, namun metode tersebut membutuhkan biaya pengoperasian yang tinggi (Irawati et al, 2012;Kurniati, 2014). Beberapa jenis bakteri diketahui sebagai agen bioremediasi cemaran merkuri pada lingkungan tanah maupun perairan.…”

Section: Pendahuluanunclassified

Uji Resistensi Bakteri Endofit Bambu terhadap Logam Merkuri dan Identifikasi Secara Molekuler dengan Analisis Gen 16S rRNA

Arifuddin

Banna

2021

Agro. Bali. Agric. J.

View full text Add to dashboard Cite

Merkuri merupakan salah satu logam berat yang berbahaya bagi lingkungan. Pemanfaatan bakteri endofit bambu sebagai bakteri pendegradasi logam merkuri belum banyak dilaporkan sehingga perlu dilakukan penelitian untuk memperoleh isolat bakteri endofit bambu resisten logam merkuri. Penelitian ini bertujuan untuk mengetahui resistensi isolat bakteri endofit bambu terhadap logam merkuri. Isolat bakteri yang digunakan merupakan koleksi isolat Laboratorium Biologi STKIP Pembangunan Indonesia, yang diberi kode KL2 A Hitam, KL2 Rehitam, KL2 Blit2, KL2 Rebatik. Pada uji resistensi, sebanyak 100 µL larutan HgCl2 diteteskan pada paper disk yang ditumbuhkan pada media Nutrient Agar sebagai media tumbuh bakteri endofit bambu selama 24 jam pada suhu 37 °C. Konsentrasi HgCl2 yang digunakan adalah 10, 20, 30, 40 dan 50 ppm. Hasil menunjukkan keempat isolat bakteri dapat tumbuh pada seluruh media yang mengandung larutan HgCl2, yang ditandai dengan terbentuknya zona bening di sekitar paper disk. Berdasarkan pengukuran zona bening, isolat KL2 Rebatik membentuk zona bening sebesar 13 mm pada media mengandung 10 ppm HgCl2 dan merupakan zona bening terbesar. Tiga isolat lainnya, isolat KL2 A Hitam, KL2 Rehitam dan KL2 Blit2 pada media mengandung 10 ppm HgCl2 membentuk zona bening masing-masing 10,66 mm dan 7,6 mm. Seluruh isolat bakteri menunjukkan penurunan ukuran zona bening seiring dengan meningkatnya konsentrasi HgCl2 yang digunakan. Hal ini menunjukkan bahwa bakteri endofit bambu resisten terhadap ion Hg2+ hingga konsentrasi 50 ppm. Isolat KL2 Rehitam dipilih untuk diindentifikasi menggunakan amplikasi gen 16S rRNA. Hasil identifikasi menunjukkan isolat KL2 Rehitam memiliki tingkat kemiripan sebesar 99% dengan Bacillus cereus.

show abstract

A Study on Mercury-Resistant Bacteria Isolated from a Gold Mine in Pongkor Village, Bogor, Indonesia

Cited by 37 publications

References 15 publications

Mercury contamination imposes structural shift on the microbial community of an agricultural soil

Mercury contamination imposes structural shift on the microbial community of an agricultural soil

Characterizing and Evaluating the Diverse Microbial Communities and Their Mercury Resistance Potential from Hot Spring Sites Representing Gradients in Temperature and pH in Yellowstone National Park

Uji Resistensi Bakteri Endofit Bambu terhadap Logam Merkuri dan Identifikasi Secara Molekuler dengan Analisis Gen 16S rRNA

Contact Info

Product

Resources

About