A Study on Karyotype of the Asian Leopard Cat, &lt;i&gt;Prionailurus bengalensis&lt;/i&gt; (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Technique

Keawmad, Puntivar; Tanomtong, Alongklod; Khunsook, Sumpars

doi:10.1508/cytologia.72.101

Cited by 4 publications

(16 citation statements)

References 13 publications

(27 reference statements)

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“…From this study, the leopard had the chromosome marker at the short arm of chromosomes E1 (chromosome pairs 14) that was a satellite chromosomes with nucleolar organizer regions (NORs). This result agrees with previous reports about the chromosomes E1 of animals in the family Felidae having a satellite chromosomes (Makino and Tateishi 1952, Thuline and Norby 1961, Hsu 1962, Hsu et al 1963, Hsu and Rearden 1965, Ohno et al 1962, Matano 1963, Chu et al 1964, Benirschke and Low 1966, Leyhausen and Tonkin 1968, Sutton 1968, Hard 1968, Wurster and Benirschke 1967, 1968a, 1968b, Wurster 1969, Milosevic et al 1972, Wurster-Hill 1973, WursterHill and Meritt 1974, Keawmad et al 2007.…”

Section: Discussionsupporting

confidence: 92%

“…This result agrees with the previous studies by Hsu et al (1963) indicating that a member of the leopard family has 2nϭ38. This corresponds to 2n for the member of the family Felidae according to reports in puma (Felis concolor) mestic cat (F. catus) and ocelot (F. pardalis) (Makino and Tateishi 1952, Thuline and Norby 1961, Hsu 1962, Hsu et al 1963, Hsu and Rearden 1965, Ohno et al 1962, Matano 1963, Chu et al 1964, Benirschke and Low 1966, Leyhausen and Tonkin 1968, Sutton 1968, Hard 1968, Wurster and Benirschke 1967, 1968a, 1968b, Wurster 1969, Milosevic et al 1972, Wurster-Hill 1973, Wurster-Hill and Meritt 1974, Keawmad et al 2007. However this number differs from the chromosome number of Geoffroy's (F. geoffroyi) and Marguay (F. wiedi) which is 2nϭ36 (Hsu 1962, Hsu et al 1963a.…”

Section: Discussionmentioning

confidence: 67%

“…The culture cells were treated with a colchicine-hypotonic-fixation-air-drying technique followed by conventional staining, G-banding and high-resolution staining techniques with Giemsa's (Rooney 2001, Campiranon 2003. Twenty cells of each individual chromosome checks, length measurements, karyotyping and idiograming were accomplished by using light microscope as previously described (Chaiyasut 1989, Keawmad et al 2007.…”

Section: Methodsmentioning

confidence: 99%

“…There are several reports on Cytogenetic studies of the family Felidae included Makino and Tateishi (1952), Thuline and Norby (1961), Ohno et al (1962), Hsu et al (1963), Matano (1963), Chu et al (1964), Hus and Rearden (1965), Benirschke and Low (1966), Leyhausen and Tonkin (1968), Sutton (1968), Wurster and Benirschke (1967, 1968a, 1968b, Hard (1968), Wurster (1969), Milosevic et al (1972), Wurster-Hill (1973), Wurster-Hill and Meritt (1974), Yang et al (2000), Nie et al (2002), and Keawmad et al (2007). Our knowledge, cytogenetic study of the leopard, Panthera pardus (Carnivora, Felidae), can be a useful information for the conservation of family Felidae in Thailand.…”

mentioning

confidence: 99%

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Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

et al. 2008

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Summary This is a cytogenetic study of the leopard, Panthera pardus (Carnivora, Felidae). Blood samples were taken from 2 males and 1 female. After the standard whole blood lymphocyte culture in the presence of colchicine, the metaphase spreads were performed on microscopic slides and airdried. Conventional staining, G-banding and high-resolution staining technique were applied to stain the chromosomes. The results showed that 2n (diploid) of leopard was 38, and the fundamental number (NF) was 72 in the male and female. There are 6 autosome types: A type had 4 large submetacentric and 2 medium submetacentric chromosomes, B type had 4 large acrocentric and 4 medium acrocentric chromosomes, C type had 2 large metacentric and 2 medium metacentric chromosomes, D type had 8 small submetacentric chromosomes, E type had 6 small metacentric chromosomes and F type had 4 small telocentric chromosomes. A pair of the short arm of chromosome E1 (chromosome pairs 14) showed a clearly observable satellite chromosomes. The X chromosome was small submetacentric chromosome and the Y chromosome was the smallest submetacentric chromosome. From the G-banding and high-resolution staining technique, the number of bands and locations in the leopard was 163 and 191 respectively, and each chromosome pair could be clearly differentiated. We found that chromosomes A1, B3, B4, C1, C2, D1, D3, E1, E2, E3, F1, F2, X and Y chromosome patterns were according to the domestic cat (Felis catus) chromosomes. Chromosomes A2, A3, B1, B2, D2 and D4 are similar to those of the domestic cat. These results show the evolutionary relationship between the leopard and domestic cat. The karyotype formula for the male and female leopard is as follows: 2n (38)

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

et al. 2008

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“…Although cytogenetic studies of the family Felidae have been reported (Makino and Tateishi 1952, Thuline and Norby 1961, Hsu et al 1963, Hus and Rearden 1965, Ohno et al 1962, Matano 1963, Chu et al 1964, Benirschke and Low 1966, Leyhausen and Tonkin 1968, Sutton 1968, Hard 1968, Wurster and Benirschke 1967, 1968a, 1968b, Wurster 1969, Milosevic et al 1972, Wurster-Hill 1973, Wurster-Hill and Meritt 1974, Yang et al 2000, Nie et al 2002, Keawmad et al 2007, there are few reports of the cytogenetics of the family Felidae in this species in Thailand. As the clouded leopard is a highly endangered species, several follow-up are of interest for potential contribution to the conservation of this species.…”

mentioning

confidence: 98%

Standardized Karyotype and Idiogram of the Clouded Leopard, Neofelis nebulosa (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

et al. 2008

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Summary A cytogenetic study of the clouded leopard (Neofelis nebulosa) in Thailand has been made. Blood sample were taken from 1 male and 1 female. After the standard whole blood lymphocyte culture in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining, G-banding and high-resolution staining technique were applied to stain the chromosomes. The results showed that 2n (diploid) of clouded leopard was 38, and the fundamental number (NF) was 74 in the male and female. There are 6 autosome types: A type had 4 large and 2 medium submetacentric chromosomes, B type had 6 large and 2 medium acrocentric chromosomes, C type had 4 large metacentric chromosomes, D type had 8 small submetacentric chromosomes, E type had 8 small metacentric chromosomes and F type had 2 small telocentric chromosomes. A pair of the short arm of chromosome E1 (chromosome pairs 14) showed a clearly observable satellite chromosomes. The X chromosome was medium submetacentric chromosome and the Y chromosome was the smallest submetacentric chromosome. From the G-banding and high-resolution technique, the number of bands and locations in the clouded leopard was 178 and 222 respectively, and each chromosome pair could be clearly differentiated. We found that chromosomes A1, B3, B4, C1, C2, D1, D4, E1, E3, F1 and X-chromosome patterns were according to the domestic cat (Felis catus) chromosomes. Chromosomes A2, A3, D2, D3, E2 and Y-chromosome are similar to those of the domestic cat. These results show the evolutionary relationship between the clouded leopard and domestic cat. The karyotype formula for the male and female clouded leopard is as follows: 2n(38)ϭL 4

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Karyological Study of the Jungle Cat, Felis chaus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

et al. 2008

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SummaryAs an endangered species in Thailand, wild animal species of the jungle cat (Felis chaus) was selected for karyological study. Blood samples were taken from 2 males and 2 females. After the standard whole blood lymphocyte culture in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining, G-banding and high-resolution staining technique were applied to stain the chromosomes. The results showed that 2n (diploid) of jungle cat was 38, and the fundamental number (NF) was 72 in the male and female. There are 6 autosome types: A type had 6 large submetacentric chromosomes, B type had 8 large acrocentric chromosomes, C type had 4 large metacentric chromosomes, D type had 8 small submetacentric chromosomes, E type had 6 small metacentric chromosomes and F type had 4 small telocentric chromosomes. A pair of the short arm of chromosome E1 (chromosome pairs 14) showed a clearly observable satellite chromosomes. The X chromosome was medium submetacentric chromosome and the Y chromosome was the smallest submetacentric chromosome. From the G-banding and high-resolution staining technique, the number of bands and locations in the jungle cat was 167 and 183 respectively, and each chromosome pair could be clearly differentiated. We found that chromosomes B1, B2, D1, D4, E3, F2 and X chromosome patterns were according to the domestic cat (Felis catus) chromosomes. Chromosomes A1, A2, A3, B3, B4, C1, C2, D2, E1, E2, F1 and Y-chromosome are similar to those of the domestic cat. These results show the evolutionary relationship between the jungle cat and domestic cat. The karyotype formula for the male and female jungle cat is as follows: 2n (38)

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A Study on Karyotype of the Asian Leopard Cat, <i>Prionailurus bengalensis</i> (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Technique

Cited by 4 publications

References 13 publications

Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

Cytogenetic Study of the Leopard, Panthera pardus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

Standardized Karyotype and Idiogram of the Clouded Leopard, Neofelis nebulosa (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

Karyological Study of the Jungle Cat, Felis chaus (Carnivora, Felidae) by Conventional Staining, G-banding and High-resolution Staining Technique

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