A Study on Fucosyltransferase 2 Gene Polymorphism and Secretion Status Related to Neonatal Necrotizing Enterocolitis

Abstract

Objective. To detect the single nucleotide polymorphism (SNP) of alpha-(1,2) fucosyltransferase 2 gene (FUT2) and the secretion status in the newborns of Chongqing China and explore the relationship between genotype or phenotype of FUT2 and neonatal necrotizing enterocolitis (NEC). Methods. Newborns who were hospitalized in Children’s Hospital of Chongqing Medical University from August 2014 to December 2015 and in line with the inclusion criteria were chosen as the research subjects; 34 cases of them in accordance with the diagnostic criteria of NEC stage II or III were NEC group, and 36 other cases of them were the control group. Total DNA was extracted from oral epithelial cells of patients which were collected with cotton buds. FUT2 SNP genotype was detected by gene sequencing. H antigen was detected with saliva samples by saliva agglutination inhibition test. Related clinical data were collected for analysis. Results. There are three genotypes on the rs1047781 (A385T) allele of the FUT2 encoding sequence: AA, AT, and TT. The number of genotypes AA, AT, and TT in the NEC group was 9 (26.47%), 12 (35.29%), and 13 (38.24%), respectively. In the control group, the number of genotypes AA, AT, and TT was 12 (33.33%), 17 (47.23%), and 7 (19.44%). There were no differences in genotypes between the two groups according to the chi-square test ( P > 0.05 ). There were 22 cases of secretors (64.7%) and 12 cases of nonsecretors (35.3%) in the NEC group. The number of secretors and nonsecretors in the control group was 31 (88.89%) and 5 (11.11%). Statistical difference was found in the phenotype between two groups through the chi-square test ( P < 0.05 ). In addition, no G428A homozygous mutation, which causes nonsecretor phenotype in Caucasians, was seen in all the subjects of this study. Conclusions. These findings indicate that secretion status (nonsecretor) was significantly associated with NEC in Chongqing, China.

Tumor Region Location and Classification Based on Fuzzy Logic and Region Merging Image Segmentation Algorithm

“…In order to simplify the subsequent segmentation task, we can cut out a small region from the global. In training, the region is generated from the standard data, while in testing, the region is generated from the result of the previous segmentation task [ 10 , 11 ].…”

Role of Bifidobacteria on Infant Health

“…Moreover, observational studies have linked the loss of Bifidobacterium in infants and enteric inflammation early in life [24]. Additionally, some studies associate the prevalence of NEC in premature infants carrying a mutation in the FUT2 gene (non-secretor genotype), which encodes a glycosyltransferase, with a lower abundance of bifidobacteria [90,91].…”

Fucosyltransferase 2: A Genetic Risk Factor for Intestinal Diseases

“…In fact, HBGA can emerge from multiple organs in vivo probably. The reason is that the main function of the FUT1 gene (H) is to control the form of antigen type 2 on the red blood cell; nevertheless, the FUT2 gene (Se) mainly catalyzes the type 1 antigens of other mucosal tissues, body fluids, such as in saliva, breast milk and digestive juices (Ye and Yu, 2021 ), and epithelial cells. Therefore, we can determine, theoretically, when both FUT1 and FUT2 genes have normal expression functions, namely, the secretors genotype.…”

“…Due to the association with HBGA and enteric virus, GWAS has demonstrated the association between IBD and single nucleotide polymorphism (SNPs) of the FUT2 and FUT3 genes, which drive the synthesis of HBGA, and ligands for NoV and RV in the intestine (Tarris et al, 2021 ). SNPs can determine the secretion status of FUT2 gene, such as G428A (Caucasians population) (Soejima and Koda, 2021a ), 778C>Del (Soejima and Koda, 2021a ), A385T (Asian population) (Ye and Yu, 2021 ), 818C>A (Thr273Asn) (Soejima et al, 2012 ), 853G>A (Ala285Thr) (Soejima et al, 2012 ), 617 T>G (V206G) (Tian et al, 2014 ), 841G>A (G281R) (Tian et al, 2014 ), 244G>A (Soejima et al, 2008 ), 569G>A (Soejima et al, 2008 ), 950C>T (Soejima et al, 2008 ), 357C>T; 856T>C; and 863C>T (Soejima and Koda, 2021b ); both are mutations that inactivate the enzyme and thus result in loss of Se enzyme activity, which mainly determines the non-secretor type. However, this is not a universal phenomenon in populations around the world, as SNPs of FUT2 are specific for population and region.…”