A study on FoxP3 and Tregs in paired samples of peripheral blood and synovium in rheumatoid arthritis

Shalini, P. V.; Debnath, Tanya; Jvs, Vidyasagar; Kona, Lakshmi; Kamaraju, Suguna R.; Kancherla, Ravindranath; Chelluri, Lakshmi Kiran

doi:10.5114/ceji.2015.55872

Cited by 5 publications

(2 citation statements)

References 18 publications

(18 reference statements)

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“…to compensate for the excess activation of effector cells. This hypothesis was supported by the raised concentrations of CCR4 + -a chemokine receptor that enables homing to inflammation foci, and HLA-DR + Treg cells with higher FoxP3 expression, as well as by the elevated expression of PD-L1 in patients with RA-a finding consistent with previous studies ( 37-39 ). Lower CTLA-4 expression on the surface of Tregs in RA has been hypothesized to be associated with a reduction in suppressor activity ( 40 , 41 ); however, it might also indicate a greater Treg involvement in contact with APCs.…”

Section: Discussionsupporting

confidence: 88%

T‑regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro

et al. 2021

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Section: Discussionsupporting

confidence: 88%

T‑regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro

et al. 2021

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“…On the other hand, regulatory T cells (Tregs) expressing CD4, CD25 and Foxp3 molecules can recognize alloantigens and induce transplantation tolerance. These cells have the ability to suppress immune responses both in vitro and in vivo [15, 16]. Tregs inhibit T cell proliferative responses through cell–cell contact or by secretion of suppressive cytokines such as IL-10 and/or TGF-β.…”

Section: Introductionmentioning

confidence: 99%

Fas, FasL and Foxp3 gene expression in post-liver transplant autoimmune hepatitis patients with and without acute rejection

Shams

Kalantar

Karimi

et al. 2019

ceh

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Aim of the study In this study we investigated Fas, FasL and Foxp3 expression in relation to liver graft rejection and its severity in autoimmune hepatitis (AIH) patients. Material and methods Twenty-three AIH patients including five post-transplant patients with acute rejection (AR) and 18 patients without AR (non-AR) were studied for Fas, FasL and Foxp3 gene expression in peripheral blood mononuclear cells on days 1, 3 and 7 after transplantation by real-time PCR. The relationships between gene expression and clinical features were determined. Results Real-time PCR showed various Fas gene expression levels with no significant difference between the days in AR patients ( p = 0.52). In non-AR patients, Fas level increased from 0.98 ±0.24 fold on the first day to 1.89 ±0.42 fold on day 3 after transplantation ( p < 0.01). In this group of patients, we also found a significant increase in FasL expression on day 7 (29.91 ±6.89 fold) compared to day 1 (13.50 ±7.44 fold, p < 0.05). Foxp3 gene expression in both groups showed decreased levels during the first week after transplantation. The decreased Foxp3 expression in AR patients was correlated with rejection activity index ( r = 0.86, p < 0.0001). Conclusions Increased Fas and FasL gene expression levels in non-AR patients and decreased Foxp3 gene expression in both groups suggested the important role of these molecules in the alloreactive response after liver transplantation in AIH patients. Foxp3 expression might be useful for monitoring rejection severity.

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Trivalent chromium supplementation ameliorates adjuvant induced rheumatoid arthritis through up-regulation of FOXP3 and decrease in synovial Cathepsin G expression

et al. 2022

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Background Rheumatoid arthritis (RA) is a known debilitating autoimmune disease. Immune-suppressants that are used for disease treatment have serious side effects, therefore, trivalent chromium (Cr (III)); which has shown evidence of its influences on some inflammatory pathways and cytokines; was used in this study for the first time to be assessed for its therapeutic effect in RA rat model and was compared to prednisolone in a trial to find a treatment with lesser side effects. Methods Adult male albino rats were randomly divided into four groups: normal, untreated RA, prednisolone treated RA (1.25 mg/kg/day) and Cr (III) treated RA groups (80 μg/kg/day), induction of RA was done by subcutaneous complete Freund adjuvant injection. Study duration was 4 weeks throughout which arthritis scoring and weight measurement were pursued. Histopathological examination and immunohistochemical FOXP3 assessment were done for joint biopsies. Serum inflammatory markers (interleukin 17, interleukin 10, CRP) and synovial erosive arthritis marker (Cathepsin G) were measured. HDL and non-HDL cholesterol were estimated as well. Results Cr (III) treatment showed marked clinical and histopathological improvement, also astonishing anti-inflammatory effects (increase in FOXP3 expression and interleukin 10, with decrease in interleukin 17, CRP and synovial Cathepsin G) to the extent that Cr (III) effects on inflammation abolishment were comparable to that of prednisolone and even better at some aspects. Moreover, Cr (III) was protective from side effects, i.e., weight gain and dyslipidemia that were seen with prednisolone treatment. Conclusions Cr (III) is promising in treating RA and it lacks some side effects of accustomed immune-modulatory agents including prednisolone. Further experimental studies and clinical trials should be held to see the efficacy of Cr (III) in different doses and to assess its long term side effects when used for rheumatoid arthritis and other autoimmune diseases treatment.

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A study on FoxP3 and Tregs in paired samples of peripheral blood and synovium in rheumatoid arthritis

Cited by 5 publications

References 18 publications

T‑regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro

T‑regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro

Fas, FasL and Foxp3 gene expression in post-liver transplant autoimmune hepatitis patients with and without acute rejection

Trivalent chromium supplementation ameliorates adjuvant induced rheumatoid arthritis through up-regulation of FOXP3 and decrease in synovial Cathepsin G expression

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