A study on factors affecting propagation of shade plant- Syngonium podophyllum .

Hassanein, A.

doi:10.37855/jah.2004.v06i01.06

Cited by 1 publication

(7 citation statements)

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“…Meristematic domes with the first or second leaf primordia at a maximum size of 0.2 mm were taken under stereomicroscope in sterile conditions during springtime. Syngonium in vitro cultures have also been used by other professional groups [22,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] (see Table 1). In 1976, Lynn Miller and Toshio Murashige published the first protocol for micropropagation of Syngonium, starting with 0.2-0.4 mm axillary meristems taken under stereomicroscope [22].…”

Section: In Vitro Culture Initiationmentioning

confidence: 99%

“…For example, Lynn Miller and Toshio Murashige used a hormone balance in favor of auxin (i.e., 2 mg of 2iP and 3 mg of IAA) [22]. Other teams used a balance in favor of cytokinin [49,51,52,[56][57][58][59]. Later, other authors used new synthetic regulatory substances, again more inclined towards cytokinin, such as 1 mg of NAA and 2 mg of (2-chloro-4-pyridyl)-N -phenylurea (CPPU) or 2 mg of N-phenyl-N -1,2,3-thiadiazol-5-ylurea (TDZ) [56,59] (see Table 1).…”

Section: In Vitro Culture Initiationmentioning

confidence: 99%

“…Moreover, other authors were successful in initiating and studying callus formation based on a specific hormone balance [46,49,[51][52][53]56,63]. However, it should be noted that a burst of phenolic compounds may also affect callus formation [75].…”

Section: Callus Initiation From Fragmented Petioles and Leavesmentioning

confidence: 99%

“…The lack of callus formation in fragmented leaves under our experimental conditions may have been due to the culture medium composition, which was not supportive of the expression of hypertrophy, although it was proven to express the highest peroxidase activity. This subject should be of interest when investigating callus initiation for different purposes [46,49,[51][52][53]56,63].…”

Section: Callus Initiation From Fragmented Petioles and Leavesmentioning

confidence: 99%

“…The histological study of the callus may provide new insights regarding th needed for de novo development of the shoots [31,46,48,49,[51][52][53]57,61]. More studying the wound stress of the species, we may provide further evidence for vance of the hormone balance used for initiating in vitro callus development [ chain of events consisting of increasing the peroxidase activity, hypertrophy of th sites, and callus formation starting from the cambium in petioles is already consist findings for other studied plant species [19,54].…”

Section: Effects Of Cysteine On Callus Formation In N69 Mediummentioning

confidence: 99%

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De Novo Shoot Development of Tropical Plants: New Insights for Syngonium podophyllum Schott.

Sand

Antofie

2022

Horticulturae

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Syngonium podophyllum Schott. cv. ‘White Butterfly’ is recognized as a valuable ornamental plant, and today it is also an important plant species of medicinal interest due to its high contents of phenolic compounds. The purpose of this article is to review the main scientific publications from our laboratory with regard to new scientific achievements dealing with Syngonium species or topics of interest, such as callus formation and further de novo shoot regeneration. The principles and stages necessary to start an industrial-level micropropagation protocol are discussed based on our experience. Different media compositions induced different morphogenetic responses inside the callus—particularly those related to the development of xylematic elements in the organogenetic areas, such as those for rooting, protocorms, and de novo shoot formation. The re-evaluation of old histological images revealed for the first time that xylematic elements are constantly closely positioned to all organogenetic centers, and that their development is closely dependent on the composition of the culture medium. Separate protocorms can be identified only when xylematic tracheary elements are well developed and closely connected to them. The formation of protocorms is strongly dependent on the mineral composition of the culture medium and the balance of plant growth regulators.

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