A Study on Development of High Functional Materials Producing Technique using By-products from Skate Processing (1) - Development of Chondroitin Sulfate Materials using Skate Cartilages

Baek, Jang-Mi; Kang, Keon-Hee; Kim, Sang-Ho; Noh, Jeong-Sook; Jeong, Kap-Seop

doi:10.5322/jesi.2016.25.5.645

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The SCS was provided by Yeongsan Skate (Yeongsan Skate Co., Ltd., Busan, Korea). Skate cartilage was obtained during the skate processing as mentioned in the previous study [22].…”

Section: Preparation Of Skate Cartilage Extracts Containing Chondroitin Sulfatementioning

confidence: 99%

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Skate cartilage extracts containing chondroitin sulfate ameliorates hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet-fed LDL receptor knockout mice in comparison with shark chondroitin sulfate

et al. 2020

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BACKGROUND/OBJECTIVES:In this study, we investigated the beneficial effects of skate cartilage extracts containing chondroitin sulfate (SCS) on hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet (HCD)-fed mice in comparison with the effects of shark cartilage-derived chondroitin sulfate (CS). MATERIALS/METHODS: Low-density lipoprotein receptor knockout (LDLR-KO) mice were fed HCD with an oral administration of CS (50 and 100 mg/kg BW/day), SCS (100 and 200 mg/kg BW/day), or water, respectively, for ten weeks. RESULTS: The administration of CS or SCS reduced the levels of serum triglyceride (TG), total cholesterol (TC), and LDL cholesterol and elevated the levels of high-density lipoprotein cholesterol, compared with those of the control group (P < 0.05). Furthermore, CS or SCS significantly attenuated inflammation by reducing the serum levels of interleukin (IL)-1β and hepatic protein expression levels of nuclear factor kappa B, inducible nitric oxide synthase, cyclooxygenase-2, and IL-1beta (P < 0.05). In particular, the serum level of tumor necrosis factor-alpha was reduced only in the 100 mg/kg BW/day of SCS-fed group, whereas the IL-6 level was reduced in the 100 and 200 mg/kg BW/day of SCS-fed groups (P < 0.05). In addition, lipid peroxidation and nitric oxide production were attenuated in the livers of the CS and SCS groups mediated by the upregulation of hepatic proteins of antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase (P < 0.05). CONCLUSIONS: These results suggest that the biological effects of SCS, similar to those of CS, are attributed to improved lipid profiles as well as suppressed inflammation and oxidative stress induced by the intake of HCD.

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“…The SCS was provided by Yeongsan Skate (Yeongsan Skate Co., Ltd., Busan, Korea). Skate cartilage was obtained during the skate processing as mentioned in the previous study [22].…”

Section: Preparation Of Skate Cartilage Extracts Containing Chondroitin Sulfatementioning

confidence: 99%

“…Only the fillet of the skate is consumed due to its unique odor; the remaining parts (e.g. skin, cartilage, and bone) weighing about 30%, are discarded [22]. We previously investigated the protective effects of skate cartilage extracts containing chondroitin sulfate (SCS) in lipopolysaccharide (LPS)-induced liver damage [18].…”

Section: Introductionmentioning

confidence: 99%

Skate cartilage extracts containing chondroitin sulfate ameliorates hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet-fed LDL receptor knockout mice in comparison with shark chondroitin sulfate

et al. 2020

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Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

Kim¹,

Lee²,

Shin³

et al. 2022

Korean J. Food Preserv.

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In this study, we developed and validated a high-performance liquid chromatography/photodiode array method for the quantification of glycine-proline-hydroxyproline (GPH) as a marker compound in skate skin collagen peptides (SCPs). The accurate molecular mass and amino acid sequence of this marker were determined using a QTOF mass spectrometer. Chromatographic separations were conducted using a 95:5 isocratic mobile phase of 0.1% trifluoroacetic acid (A) and acetonitrile/methanol (1:4 v/v, B) at a flow rate of 0.3 mL/min; the separated compounds were detected at 214 nm using a Jupiter® 4 μm Proteo 90Å column. The method was validated for specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. It was found to be linear in the range of 1.0-50.0 μg/mL, with a good correlation coefficient (R2=0.9999) and excellent specificity. The LOD and LOQ were 0.07 and 0.22 μg/mL, respectively. A recovery study determined the accuracy of the method, with an average recovery of 103.76%, 100.35% and 103.97% of the marker at 10, 15 and 25 μg/mL, respectively, and a precision study showed a relative standard deviation of less than 2%. Additionally, the DPPH and ABTS radical scavenging activities of the SCPs increased in a concentration-dependent manner, and UV-protective effect was confirmed by human keratinocyte (HaCaT cell) viability. Our study thus provides the foundation for developing functional foods or cosmetics using SCPs.

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A Study on Development of High Functional Materials Producing Technique using By-products from Skate Processing (1) - Development of Chondroitin Sulfate Materials using Skate Cartilages

Cited by 2 publications

References 13 publications

Skate cartilage extracts containing chondroitin sulfate ameliorates hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet-fed LDL receptor knockout mice in comparison with shark chondroitin sulfate

Skate cartilage extracts containing chondroitin sulfate ameliorates hyperlipidemia-induced inflammation and oxidative stress in high cholesterol diet-fed LDL receptor knockout mice in comparison with shark chondroitin sulfate

Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

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