A study on co-localization of FSH and its receptor in rat hippocampus

Abstract

It has been known that GnRH, LH and their receptors exist in hippocampal neurons. However, whether FSH and its receptor also exist in hippocampal neurons remained unknown yet. In situ hybridization, double-labeled immunofluorescence stain and double-labeled immunohistochemistry stain in adjacent sections were used in our research to study the distribution, co-localization of FSH and its receptor and co-localization of FSH and GnRH receptor in rat hippocampus. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. These suggested that hippocampal neurons not only express FSH but also act as FSH target cells. FSH may regulate the function of hippocampal neurons by ways of paracrine or autocrine. At the same time, GnRH may regulate the function of FSH neuron in hippocampus through GnRH receptor.

Presence of β-Follicle-Stimulating Hormone and β-Luteinizing Hormone Transcripts in the Brain of <i>Cichlasoma dimerus</i> (Perciformes: Cichlidae)

“…This probably explains the difficulty in the detection of FSH cells by IHC using heterologus antisera in some species [16, 21]. The expression of β-LH and β-FSH transcripts in the brain of C. dimerus was consistent with their peptide immunoreactivity previously shown in this species [11], other teleost fish [8, 9] and mammals [3, 4]. In the tilapia O. mossambicus, β-FSH and LH subunits were also cloned and partially sequenced in the preoptic nucleus [9].…”

“…It was also shown that LH was present in the rat amygdala, septal area, thalamus, caudate nucleus and hippocampus [3]. Recently, a colocalization of FSH and its receptor in the rat hippocampus was demonstrated [4]. …”

GnRH analogue attenuated apoptosis of rat hippocampal neuron after ischemia–reperfusion injury

“…There are complex inter-regulation mechanisms in these hormones. Our previous study found that not only FSH and its receptor but also FSH and GnRH receptor co-located in pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus, which suggested that FSH might regulate the function of hippocampal neurons by ways of paracrine or autocrine and GnRH might regulate the function of FSH positive neurons in hippocampus through GnRH receptor (Chu et al 2008a). It has been known that GnRH, FSH and estrogen exist in hippocampal neurons together with their receptors.…”

Extrapituitary production of anterior pituitary hormones: an overview

“…Although brain receptors for FSH were previously thought to be absent [141], they have now been reported to be present in the hippocampus [151], suggesting functional significance. Indeed, as hippocampal neurons are extrapituitary sites of FSH expression [150], FSH is likely to have local autocrine or paracrine roles.…”

“…There is a scarcity of reports on the occurrence of extrapituitary follicle-stimulating hormone (FSH), however, FSH Endocrine (2012) 41:19-30 23 protein and its transcripts have been shown to be present in the brain [142], particularly the preoptic area [143], the hypothalamus [150] and the hippocampus [151]. Although brain receptors for FSH were previously thought to be absent [141], they have now been reported to be present in the hippocampus [151], suggesting functional significance.…”