This study was to identify factors influencing the job-related burnout of the nurses and caregivers in nursing homes. The participants in this study were 188 who were working as nurses (77) and caregivers (111) at five nursing homes in Seoul and Gyeonggi Province. Collected data were analyzed in independent t-test, Pearson's correlation coefficients, multiple stepwise regression using IBM SPSS Statistics 19.0. The nurse's job-related burnout was positively correlated with job-satisfaction(r=.333, p=.003), and stress(r=.484, p<.001), whereas caregiver's job-related burnout was positively correlated with stress(r=.458, p<.001). The factor influencing on nurse's job-related burnout was interpersonal relation stress and this factors explained 27.0% of the variance. In case of the caregivers, work-related stress was a factor influencing on job-related burnout and it explained 21.2% of the variance. On the basis of the results in this study, the improvement of working conditions, and the development of stress management program should be considered systematically.