A Study on Antioxidant Activity and Antioxidant Compound Content by the Types of Tea

Kim, Myeong-Gil; Oh, Moon-Seog; Jeon, Jong‐Sup; Kim, Han-Taek; Yoon, Mi‐Hye

doi:10.13103/jfhs.2016.31.2.132

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…The results revealed that the iNOS and COX-2 expression levels were concentration-dependent and significantly decreased at DOE concentrations of 200 and 400 μg/mL, respectively (Figs. 10,11); IL-1β was concentration-dependent and significantly decreased at a DOE concentration > 200 μg/mL; and IL-6 and TNF-α were concentration-dependent and significantly decreased at a DOE concentration of 100 μg/mL or higher (Figs. [12][13][14].…”

Section: Jarmentioning

confidence: 97%

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A Study of the Antioxidant and Anti-Inflammatory Effects of Dusokohwaeum

Seon,

Jeong,

Yoon

et al. 2023

J Acupunct Res

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Background:The aim of this study is to determine the antioxidant and anti-inflammatory effects of Dusokohwaeum (DOE). Methods: To measure the antioxidant and anti-inflammatory effects of DOE, the total flavonoid and polyphenol contents and radical scavenging activity were measured. Furthermore, reactive oxygen species (ROS), nitric oxide, and cytokine production were measured by treating lipopolysaccharide-induced RAW264.7 cells with DOE, and gene expression levels of inducible cyclooxygenase-2, nitric oxide synthase, and cytokines were evaluated. Results: Radical scavenging experiments revealed a significant concentration-dependent increase in scavenging capacity. The production of ROS, nitric oxide, and cytokines in the cells showed a significant concentration-dependent decrease when compared with the control group. The gene expression levels of inducible cyclooxygenase-2, nitric oxide synthase, and cytokines also showed a significant concentration-dependent decrease when compared with the control group. Conclusion: Interestingly, the antioxidant and anti-inflammatory effects of DOE were 23.42 ± 0.64 mg GAE/g and 20.83 ± 0.98 mg QE/g, respectively. The administration of DOE resulted in a concentration-dependent increase in scavenging ability in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability experiments. The production of intracellular ROS and nitric oxide was significantly reduced in the presence of DOE. The production of inflammatory cytokines (prostaglandin E 2 , tumor necrosis factor-alpha [TNF-α], interleukin-1 beta [IL-1β], and IL-6) was significantly reduced in the presence of DOE. Finally, the expression levels of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, and IL-6 were significantly decreased in the presence of DOE.

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Section: Jarmentioning

confidence: 97%

“…Moreover, it improves neurodegenerative diseases, regulates gene expression, and balances the microorganisms in the body. Polyphenols can be broadly divided into flavonoids and nonflavonoids [10]. Flavonoids are secondary plant metabolites and among the most common polyphenol compounds ingested by humans.…”

Section: Jarmentioning

confidence: 99%

A Study of the Antioxidant and Anti-Inflammatory Effects of Dusokohwaeum

Seon,

Jeong,

Yoon

et al. 2023

J Acupunct Res

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Plants from Ulleung island ameliorate lipid accumulation and oxidative stress in oleic acid-induced in HepG2 Cells

Kim¹,

Kim²

2020

Korean J. Food Preserv.

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Oxidative stress can directly damage lipids, proteins, and DNA, which promotes the progression from steatosis to nonalcoholic fatty liver disease. This study aimed to determine the antioxidative effects of Campanula takesimana Nakai, Ilex integra THUNB., Indigofera pseudotinctoria Matsum., Rubus takesimensis Nakai, and Tsuga sieboldii from Ulleung Island and their ability to inhibit lipid accumulation in HepG2 cells. The total phenolics (TPC) and flavonoid (TFC) contents of extracts of the five plants were determined, and their antioxidative activities were analyzed using DPPH and ABTS radical scavenging ability, Fe 2+ reducing capacity (FRAP), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid assays. Lipid accumulation was evaluated by Oil Red O staining and intracellular triglyceride (TG) assays. Rubus takesimensis Nakai had the highest TPC among the five plants. The antioxidant activity of R. Nakai was DPPH (IC 50 =0.30 mg/mL), ABTS (IC 50 =0.33 mg/mL), FRAP (318.61 μmol FeSO 4 /g), and ORAC (1.74 μmol TE/g). The lipid peroxidation inhibitory effect of R. Nakai was reduced to 52.51% at 1 mg/mL. Incubating HepG2 cells with extracts of the five plants reduced lipid and TG accumulation by >50%, and inhibited lipid peroxidation by ~19%. These results provided evidence for the development of functional foods based on these five plants from Ulleung Island, due to their antioxidant activities and ability to reduce lipid accumulation.

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