A Study on Acinetobacter baumannii and Staphylococcus aureus Strains Recovered from the Same Infection Site of a Diabetic Patient

Castellanos, Nancy; Nakanouchi, Jun; Yüzen, Dennis; Fung, Sammie; Fernandez, Jennifer S.; Barberis, Claudia; Tuchscherr, Lorena; Ramírez, María Soledad

doi:10.1007/s00284-019-01696-7

Cited by 25 publications

(18 citation statements)

References 22 publications

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“…Cell-free culture media (CFCM) was obtained as previously described by allowing A. baumannii strains to grow for 48 h at 37°C under shaking conditions (200 rpm) in LB, PF, LB + 1% pyruvate (PYR), PF + 1% PYR, LB + 0.02% phenylalanine (PA), PF + 0.02% PA, LB + 0.02% PAA, PF + 0.02% PAA, LB + 0.02% phenylpyruvate (PP), PF + 0.02% PP, and culture (Ramsey et al, 2016; Castellanos et al, 2019). At the end of the incubation period, bacterial cultures were centrifuged for 10 min at 7,000 × g .…”

Section: Methodsmentioning

confidence: 99%

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

et al. 2019

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Acinetobacter baumannii ( Ab ) is one of the most treacherous pathogens among those causing hospital-acquired pneumonia (HAP). A. baumannii possesses an adaptable physiology, seen not only in its antibiotic resistance and virulence phenotypes but also in its metabolic versatility. In this study, we observed that A. baumannii undergoes global transcriptional changes in response to human pleural fluid (PF), a key host-derived environmental signal. Differential gene expression analyses combined with experimental approaches revealed changes in A. baumannii metabolism, affecting cytotoxicity, persistence, bacterial killing, and chemotaxis. Over 1,220 genes representing 55% of the differentially expressed transcriptomic data corresponded to metabolic processes, including the upregulation of glutamate, short chain fatty acid, and styrene metabolism. We observed an upregulation by 1.83- and 2.61-fold of the pyruvate dehydrogenase complex subunits E3 and E2, respectively. We also found that pyruvate (PYR), in conjunction with PF, triggers an A. baumannii pathogenic behavior that adversely impacts human epithelial cell viability. Interestingly, PF also amplified A. baumannii cytotoxicity against murine macrophages, suggesting an immune evasion strategy implemented by A. baumannii . Moreover, we uncovered opposing metabolic strategies dependent on the degree of pathogenicity of the strains, where less pathogenic strains demonstrated greater utilization of PYR to promote persister formation in the presence of PF. Additionally, our transcriptomic analysis and growth studies of A. baumannii suggest the existence of an alternative phenylalanine (PA) catabolic route independent of the phenylacetic acid pathway, which converts PA to phenylpyruvate (PP) and shuttles intermediates into styrene metabolism. This alternative route promoted a neutrophil-evasive state, as PF-induced degradation of PP significantly reduced overall human neutrophil chemotaxis in ex vivo chemotactic assays. Taken together, these data highlight A. baumannii pathoadaptabililty in response to host signals and provide further insight into the role of bacterial metabolism in virulence traits, antibiotic persistence strategies, and host innate immune evasion.

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Section: Methodsmentioning

confidence: 99%

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

et al. 2019

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“…While Acinetobacter baumannii and S. aureus have been frequently co-isolated from wounds (Furuno et al, 2008;Castellanos et al, 2019), to our knowledge there is no published evidence that A. baumannii possesses any inhibitory activity against S. aureus. Thus, we were surprised that A. baumannii isolates represented ∼20% (7/34) of the clinical isolates that showed anti-S. aureus activity (Figures 2A, 3).…”

Section: Heterotypic Inhibitory Activity Of a Baumannii Against S Amentioning

confidence: 95%

Antimicrobial Activity of Clinically Isolated Bacterial Species Against Staphylococcus aureus

et al. 2020

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“…The results showed that the antibiotic dosage needed to kill biofilm was more than 5000 times that needed to kill planktonic bacteria. The enhancement of drug resistance increases the difficulty of removing biofilm, which often leads to the occurrence and recurrence of clinical chronic multiple infections (23)(24)(25)(26)(27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Inhibition characteristics of biofilm structure of Staphylococcus aureus

Zhang

Mao

Sun

2020

Cell Mol Biol (Noisy-le-grand)

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Different extracts have different effects on the biofilm structure of Staphylococcus aureus, and the biofilm structure of Staphylococcus aureus will produce different inhibition reactions. In this study, different experimental reagent extracts were used to analyze the inhibition characteristics of Staphylococcus aureus biofilm structure. The inhibition characteristics of bacterial biofilm structure were obtained by using the same bacteria species and the same experimental environment. The results showed that the chloroform extract had a good inhibitory effect on the biofilm structure, which could effectively inhibit the formation of biofilm; the acetic acid extract had an impact on the formation of biofilm, which was destructive to the biofilm; the petroleum ether extract had no effect on the formation of biofilm, that is, it had no inhibitory effect.

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A Study on Acinetobacter baumannii and Staphylococcus aureus Strains Recovered from the Same Infection Site of a Diabetic Patient

Cited by 25 publications

References 22 publications

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

Antimicrobial Activity of Clinically Isolated Bacterial Species Against Staphylococcus aureus

Inhibition characteristics of biofilm structure of Staphylococcus aureus

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