A study of vascular smooth muscle cell function under cyclic mechanical loading in a polyurethane scaffold with optimized porosity

Biodegradation and in vivo biocompatibility of a degradable, polar/hydrophobic/ionic polyurethane for tissue engineering applications

“…Although the initial porogen weight content was 75% for both of these scaffolds, it was important to measure the porosity post-leaching in order to confirm that the complete removal of the porogens could be achieved and that this would yield a scaffold of similar porosity. These porosity measurements fall within the same range as those previously reported on for the D-PHI scaffolds using a fluid displacement measurement technique based on Archimede's principle [17].…”

“…Then SBP particles (65 wt%) and polyethylene glycol ((PEG) w40 mm; 10 wt%) were added to the D-PHI (25 wt%) mixture and cured at 110 C for 24 h [17]. The cured D-PHI scaffolds were then de-salted and PEG extracted using the above protocol described for de-salting the PLGA scaffolds.…”

“…Recently, a degradable, polar/hydrophobic/ionic polyurethane (D-PHI) synthesized using lysine diisocyanate (LDI) was designed in order to yield suitable mechanical properties at elevated porosities (>80%), with interconnected pores and elastic properties appropriate for use in a TE vascular graft [16,17]. In non-porous flat film form, D-PHI was shown to induce a more wound-healing phenotype monocyte and was a less activating biomaterial (low proinflammatory cytokines) than traditional tissue culture materials such as polystyrene (TCPS) [3].…”

VUV treatment combined with mechanical strain of stretchable polymer foils resulting in cell alignment

“…Elastic polyurethanes (PU) and PU-composites are of interest as flexible and stretchable cell-culture substrates and scaffolds [1][2][3]. PU is widely used in industry, especially also in the field of medicine and biotechnology [4,5], due to its good mechanical characteristics, high chemical stability, low electrical conductivity, thermal stability and low wettability.…”

Use of monocyte/endothelial cell co-cultures (in vitro) and a subcutaneous implant mouse model (in vivo) to evaluate a degradable polar hydrophobic ionic polyurethane

“…The scaffold possesses high porosity ($75-80%) and pore interconnectivity for cell proliferation and previous studies have indicated that the scaffold was biocompatible and non-toxic to cells while possessing appropriate mechanical properties (Sharifpoor et al, 2009;Sharifpoor et al, 2010). In addition, D-PHI degraded in vivo and in vitro at a controlled rate which would allow for the time necessary for vessel regeneration (McBane et al, 2011a).…”