A study of thermal dose-induced autophagy, apoptosis and necroptosis in colon cancer cells

Mouratidis, Petros; Rivens, Ian; Haar, Gail ter

doi:10.3109/02656736.2015.1029995

Cited by 48 publications

(50 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with previous reports (18), this study demonstrates that ultrasound hyperthermia destroys the structure of the mitochondrial membrane to induce apoptosis in HN-30 and HN-13 cells. However, the mechanism by which this effect is mediated has not been identified.…”

Section: Discussionsupporting

confidence: 93%

Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma: an in vitro study

Ren¹,

Jv²,

Tian³

et al. 2017

Med Oral

View full text Add to dashboard Cite

BackgroundHyperthermia is considered an efficient complement in the treatment of head and neck squamous cell carcinoma (HNSCC). Hyperthermia induces cell apoptosis in a temperature- and time-dependent manner. However, the molecular mechanism of hyperthermia remains unclear. The aim of this study was to investigate the mechanism of apoptosis induced by ultrasound hyperthermia in HNSCC cell lines HN-30 and HN-13.Material and MethodsWe examined the dynamic changes of early apoptosis and secondary necrosis in HN-30 and HN-13 cells treated by hyperthermia at 42°C for 10 min. We further examined mitochondrial membrane potential in vitro by ultrasound hyperthermia for different heating temperatures (38-44°C, 10 min) and heating times (42°C, 10-50 min). After heating by ultrasound at 42°C for 10 min, the apoptosis index achieved its highest level at 8 h after treatment, decreased rapidly and remained constant at a reduced level at 12 h.ResultsThe level of secondary necrosis increased with the level of early apoptosis but remained at a higher level until 14 h. The level of secondary necrosis correlated with the level of early apoptosis (HN-13: r=0.7523, P=0.0030; HN-30: r=0.6510, P=0.016). The fractions of cells with low mitochondrial membrane potential (Δψ) in the heating-temperature grads group and heating-time grads group decreased significantly over time. Therefore, HN-30 and HN-13 cells developed apoptosis after ultrasound hyperthermia treatment with decreases in the mitochondrial transmembrane potential level.ConclusionsUltrasound hyperthermia induces apoptosis in HN-30 and HN-13 cells, possibly via the mitochondrial caspase pathway. Key words:Head and neck squamous cell carcinoma, apoptosis, mitochondrial membrane potential, ultrasound hyperthermia.

show abstract

Section: Discussionsupporting

confidence: 93%

Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma: an in vitro study

Ren¹,

Jv²,

Tian³

et al. 2017

Med Oral

View full text Add to dashboard Cite

show abstract

“…Hyperthermia injures or kills cancer cells by disrupting the integrity of cell membrane, cytoskeleton and the mitochondrial machinery that leads to cell necrosis, or activating apoptosis and cell cycle arrest [4–6, 44]. Hyperthermia in combination with radiotherapies or chemotherapies has shown more promising clinical efficacy in treating various cancers, as the combination therapy leads to accumulation of cell toxicity, as well as increased blood flow and drug delivery [14, 15, 17, 18].…”

Section: Discussionmentioning

confidence: 99%

“…A temperature range of 42°C to 45°C is used therapeutically as a single agent or as an adjuvant to radiotherapy, chemotherapy or immunotherapy in the cancer treatment [2, 3]. Hyperthermia alone as a cancer treatment can selectively eliminate cancer cells by facilitating molecular mechanisms such as cell cycle arrest, apoptosis, necrosis and autophagy [4–6], or activate NK cells or DCs which in turn stimulates anti-cancer immune responses [7, 8]. Both in vitro and in vivo studies have reported a clinical benefit from use of hyperthermia as a treatment for many cancers including melanoma [5, 9, 10], prostate cancer [11], bladder cancer [12] and glioblastoma [13].…”

Section: Introductionmentioning

confidence: 99%

HSPB1 deficiency sensitizes melanoma cells to hyperthermia induced cell death

et al. 2016

View full text Add to dashboard Cite

Hyperthermia has shown clinical potency as a single agent or as adjuvant to other therapies in cancer treatment. However, thermotolerance induced by thermosensitive genes such as the heat shock proteins can limit the efficacy of hyperthermic treatment. In the present study, we identified HSPB1 (HSP27) is hyperthermically inducible or endogenously highly expressed in both murine and human melanoma cell lines. We used a siRNA strategy to reduce HSPB1 levels and showed increased intolerance to hyperthermia via reduced cell viability and/or proliferation of cells. In the investigation of underlying mechanisms, we found knock down of HSPB1 further increased the proportion of apoptotic cells in hyperthermic treated melanoma cells when compared with either single agent alone, and both agents leaded to cell cycle arrest at G0/G1 or G2/M phases. We concluded that hyperthermia combined with silencing of HSPB1 enhanced cell death and resulted in failure to thrive in melanoma cell lines, implying the potential clinical utility of hyperthermia in combination with HSPB1 inhibition in cancer treatment.

show abstract

“…The numerical predictions are based on wellestablished calculation procedures that have been validated many times in the literature and are a result of a multi-year evolution in the methodology of numerical modelling and simulation [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39]. Additionally, there is a wealth of research which deals with simulation and experimentation on heating of carcinogenic tissues [40][41][42][43][44][45][46][47][48][49], on thermal tolerance and dosimetry of tissues (including skin) [50][51][52][53][54], on the cellular responses to heating [55,56], heating for internal but noncancerous applications [57,58], patient-specific thermal protocols [59] and reviews [60].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive method to predict and quantify scald burns from beverage spills

Abraham¹,

Nelson-Cheeseman²,

Sparrow

et al. 2016

International Journal of Hyperthermia

View full text Add to dashboard Cite

A comprehensive study was performed to quantify the risk of burns from hot beverage spills. The study was comprised of three parts. First, experiments were carried out to measure the cooling rates of beverages in a room-temperature environment by natural convection and thermal radiation. The experiments accounted for different beverage volumes, initial temperatures, cooling period between the time of service and the spill, the material which comprised the cup, the presence or absence of a cap and the presence or absence of an insulating corrugated paper sleeve. Among this list, the parameters which most influenced the temperature variation was the presence or absence of a cover or cap, the volume of the beverage and the duration of the cooling period. The second step was a series of experiments that provided temperatures at the surface of skin or skin surrogate after a spill. The experiments incorporated a single layer of cotton clothing and the exposure duration was 30 s. The outcomes of the experiments were used as input to a numerical model which calculated the temperature distribution and burn depth within tissue. Last was the implementation of the numerical model and a catalogue of burn predictions for various beverage volumes, beverage service temperatures, and durations between beverage service and spill. It is hoped that this catalogue can be used by both beverage industries and consumers to reduce the threat of burn injuries. It can also be used by treating medical professionals who can quickly estimate burn depths following a spill incident.

show abstract

A study of thermal dose-induced autophagy, apoptosis and necroptosis in colon cancer cells

Abstract: Autophagy, apoptosis and necroptosis are associated with the response of these cancer cell lines to supra-normal temperatures.

Cited by 48 publications

References 38 publications

Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma: an in vitro study

Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma: an in vitro study

HSPB1 deficiency sensitizes melanoma cells to hyperthermia induced cell death

Comprehensive method to predict and quantify scald burns from beverage spills

Contact Info

Product

Resources

About