A study of the xenoantigenicity of neonatal porcine islet‐like cell clusters (NPCC) and the efficiency of adenovirus‐mediated DAF (CD55) expression

Abstract

The origin of the antigenicity of NPCC is mainly N-linked sugars including alpha-Gal and sialic acid antigens, and NPCC expressed the transduced molecule in high efficiency by the adenovector.

Immunological Property of Antibodies against N-Glycolylneuraminic Acid Epitopes in Cytidine Monophospho–N-Acetylneuraminic Acid Hydroxylase-Deficient Mice

“…In a previous study, the expression of NeuGc epitopes on islets from adult or neonatal pigs was clearly detected, and the antigenicity of adult pig islets or neonatal porcine islet-like cell clusters was found to be mainly associated with N-linked sugars, including NeuGc epitopes, but not Gal Ags (41)(42)(43)(44). These findings led us to investigate the immunogenicity of NeuGc epitopes located on the islets by using a CMAH +/+ -to-CMAH 2/2 mouse islet transplantation model.…”

A lectin microarray study of glycoantigens in neonatal porcine islet-like cell clusters

“…NPCCs have a strong antigenicity derived from N-linked sugars, but at the same time the cell surface glycolipids of these cells are also antigenic. Available data indicate that pig sialic acids, besides the H-D antigen, are clearly antigenic to human serum [7,8].…”

“…One is the transplantation of adult pig islets (APIs) [7]. The other is the transplantation of neonatal porcine islet-like cell clusters (NPCCs) [8]. Several clinical trials [9] and preclinical experiments [10] using either type of islets are currently in progress.…”

异种胰岛移植中供体猪的筛选: 现状与进展

“…Though insulin secretion is also delayed after transplantation in vivo, the maturation time of NPIs (>4 weeks) is significantly shorter than that of ICCs (>2 months) ( Table 1). The origin of xeno-antigenicity of pig NPIs is mainly N-linked sugars including sialic acid and α-Gal antigens (up to 11%-19%) (Rayat et al, 2003;Omori et al, 2006); however, the transplanted NPIs induce a lower T-cell response than APIs in patients with insulin-dependent diabetes mellitus (Bloch et al, 1999). Recently, the growing demonstrations of prolonged diabetes reversal after transplantation of WT NPIs in immunosuppressed primates make neonatal pig an alternative source of islet grafts for future clinical application (Cardona et al, 2006;Elliott et al, 2007;Thompson et al, 2011a;Wang et al, 2011).…”