A study of the value of electrophoretic and other techniques for typing Acinetobacter calcoaceticus

Alexander, M.; Rahtnan, M.; Taylor, M. W.; Noble, W. C.

doi:10.1016/0195-6701(88)90069-2

Cited by 33 publications

(17 citation statements)

References 19 publications

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“…Previously only limited data were available to evaluate the sensitivity and reliability of SDS-PAGE with respect to existing typing methods [12,15]. In this study we have compared the effectiveness of this technique against other methods for typing isolates of the Kiebsiella pneumoniae complex.…”

Section: Discussionmentioning

confidence: 99%

“…The technique using either conventionally stained or radiolabelled proteins, has been applied increasingly to the typing of a variety of clinically important species that have included Acinetobacter calcoaceticus [12], Campylobacter pylori [13], Clostridium difficile [14,15], Enterobacter cloacae [16], methicillinresistant Staphylococcus aureus [17,18] and Providencia alcalifacienrs [19].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

Costas¹,

Holmes²,

Sloss³

1990

Epidemiol. Infect.

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SUMMARYTwenty-four cultures comprising 20 clinical isolates of 'Klebsiella aerogenes' from two hospitals, a reference strain of 'K. aerogenes' and the type strains of three other Klebsiella species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into 12 protein types. Comparison with established typing methods indicated that the level of discrimination of SDS-PAGE was similar to that achieved with conventional typing methods but the strains were grouped differently. Protein typing subdivided five serotype K3 isolates that could also be distinguished by phage typing. Conversely, three strains of protein type 11 were clearly distinguishable by both serotyping and phage typing. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of 'K. aerogenes'.

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Section: Discussionmentioning

confidence: 99%

“…The technique using either conventionally stained or radiolabelled proteins, has been applied increasingly to the typing of a variety of clinically important species that have included Acinetobacter calcoaceticus [12], Campylobacter pylori [13], Clostridium difficile [14,15], Enterobacter cloacae [16], methicillinresistant Staphylococcus aureus [17,18] and Providencia alcalifacienrs [19].…”

Section: Introductionmentioning

confidence: 99%

Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

Costas¹,

Holmes²,

Sloss³

1990

Epidemiol. Infect.

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“…Cells were harvested by centrifugation and processed with a slight modification of the procedure described by Alexander et al (1988). SDS-PAGE was performed by the method described by Laemmli (1970), with a concentration of 12% acrylamide in the running gel.…”

Section: Sds-page Protein Patternsmentioning

confidence: 99%

The Biology of Acinetobacter

Towner¹,

Bergogne-Bérézin²,

Fewson³

1991

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All rights reservedNo part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher PREFACEThe 1st International Workshop on Acinetobacter was held on 6th September, 1986, in Manchester, England, in association with the 14th International Congress of Microbiology. That occasion was so well attended and productive that there were soon discussions about how, when and where the next meeting should be held. This time, however, there was sufficient confidence to think of a more substantial meeting and to plan for the proceedings to be published. It emerged that there was wide agreement that the time was ripe to take stock of the entire biology of Acinetobacter: its occurrence and taxonomy; its molecular biology, biochemistry and physiology; its clinical importance and its industrial and commercial applications. The 2nd International Workshop on Acinetobacter took place from 6th to 7th September, 1990, at the Institut Pasteur, Paris, and was sponsored by the Federation of European Microbiological Societies. There were about 100 participants from 19 countries. The backbone of the meeting consisted of 23 plenary lectures. There were 28 posters and the meeting closed with a general discussion which went on long after the official finishing time despite all the counter-attractions of a sunny Parisian Friday afternoon. Indeed discussions continued while cruising along the Seine and while dining at the top of the Tour Montparnasse. However, the vitality and usefulness of even the most successful meeting is difficult to transmit by the printed word. The editors decided, therefore, not simply to publish the proceedings of the Workshop, but rather to commission a set of review articles with the aim of giving an up-to-date and rounded picture of our current knowledge of the genus Acinetobacter. These reviews, together with a

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“…The technique using either conventionally stained or radiolabelled proteins, has been applied increasingly to the typing of a variety of clinically important species that have included Clostridium difficile [15], methicillin-resistant Staphylococcus aureus [16,17], Enterobacter cloacae [18] and Acinetobacter calcoaceticus [19]. Preliminary studies, using one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoretic (1-D SDS-PAGE) protein patterns [20,21] and DNA restriction endonuclease digest patterns [9,22] have shown extremely good discrimination and high reproducibility for H. pylori.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of strains ofHelicobacter pyloriby numerical analysis of 1-D SDS-PAGE protein patterns: Evidence for post-treatment recrudescence

et al. 1991

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SUMMARYTwenty-three pre-and post-treatment isolates of Helicobacter pylori from the antral mucosa of eight patients with dyspepsia and gastritis were compared using 1-D SDS PAGE of proteins. The protein patterns were highly reproducible and were used as the basis for two numerical analyses. The first, based on the total protein patterns, showed that a number of the strains did not cluster with their respective patient set. This was thought to be due to differences in both mobility and intensity of proteins in the major band region. The second analysis. based on partial patterns, excluding the major band region (51-68 kDa), divided the clinical isolates into clearly defined groups corresponding to the patient sets. Although there was a degree of heterogeneity with respect to protein pattern between the pre-and post-treatment isolates of some patients, there was nonetheless clear evidence that each patient was harbouring strains of only a single type. These, results suggested that patients were not being reinfected with a different strain but that there was recrudescence of the pre-treatment strain. Protein 'fingerprints ' provided a precise and reproducible means of strain differentiation, and revealed that in each patient the same strain persisted after drug therapv even though there was marked patient-to-patient strain variation.

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A study of the value of electrophoretic and other techniques for typing Acinetobacter calcoaceticus

Cited by 33 publications

References 19 publications

Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

The Biology of Acinetobacter

Differentiation of strains ofHelicobacter pyloriby numerical analysis of 1-D SDS-PAGE protein patterns: Evidence for post-treatment recrudescence

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