A study of the treatment of cutaneous fungal infection in animal model using photoactivated composite of methylene blue and gold nanoparticle

Tawfik, Abeer Attia; Noaman, I.; El-Elsayyad, Hasan; El-Mashad, Noha; Soliman, Mona S. S.

doi:10.1016/j.pdpdt.2016.05.010

Cited by 23 publications

(9 citation statements)

References 22 publications

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“…RB has also been reported to penetrate deeper into tissue, where photochemical protein cross‐linking was observed ≈350 µm into the reticular dermis in a clinical study using a RB‐PBS solution . However, methods of enhancing RB penetration should be nonetheless considered, particularly for more severe lesions, where the use of fractional CO 2 lasers to create micropores in the nail plate or nanoparticles can better carry RB across the nail barrier . Formation of micropores may also aid the penetration of light through the nail and thus reduce the irradiation dosage.…”

Section: Discussionmentioning

confidence: 99%

Genetic Tolerance to Rose Bengal Photodynamic Therapy and Antifungal Clinical Application for Onychomycosis

Houang

Perrone

Pedrinazzi

et al. 2018

Advanced Therapeutics

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Photodynamic therapy (PDT) with rose bengal has seen increasing use in clinical applications and has shown effective antifungal capacity in vitro. However, there is limited understanding of the effects of this emerging therapy at a genetic level. A rose bengal PDT screen using a green laser (λ = 532 nm) on the entire non-essential gene library of the model organism, Saccharomyces cerevisiae, and a subsequent pilot patient study (n = 6 patients) in the treatment of onychomycosis caused by Trichophyton rubrum is reported. Of the 4800 yeast strains screened, 482 sensitive and 175 resistant strains are identified. The key biochemical pathways found to be affected included ergosterol biosynthesis, vacuolar acidification, and purine/S-adenosyl-l-methionine biosynthesis. The implications of these findings inform the clinical application of an optimized rose bengal PDT protocol involving nail treatment with a rose bengal solution (140 µm) and green light irradiation (fluence 763 J cm −2 ). All patients achieved complete cure within three to five treatment sessions in the absence of pain or other side effects. The outcome of the genetic screen may thus inform the development of more efficient clinical treatments using rose bengal PDT, as demonstrated in the successful treatment of onychomycosis.

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Section: Discussionmentioning

confidence: 99%

Genetic Tolerance to Rose Bengal Photodynamic Therapy and Antifungal Clinical Application for Onychomycosis

Houang

Perrone

Pedrinazzi

et al. 2018

Advanced Therapeutics

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“…Alessio et al investigated the interactions of the pollutants amoxicillin (AMX) and methylene blue (MB) with a simple membrane model consisting of DPPC monolayers 59 . Amoxicillin is an antibiotic of the penicillin family used to treat bacteria-related infections, and MB is a phenothiazine derivative used as a medicine 60 , among other applications 61 . Amoxicillin and MB shifted the surface pressure of DPPC monolayers to larger areas and made these monolayers more compressible, as observed in Fig.…”

Section: Summary Of Recent Resultsmentioning

confidence: 99%

Recent advances in the use of Langmuir monolayers as cell membrane models

Pereira

Oliveira

2021

Eclet. Quim. J.

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Understanding the role of biomolecules in cells at the molecular level has been the trade of Prof. Marcio Francisco Colombo and Prof. Jo�o Ruggiero Neto in their carriers, which is why it was found appropriate to address the use of Langmuir monolayers as cell membrane models in this special issue. In the review paper, we elaborate upon the reasons why Langmuir monolayers are good models with the possible control of membrane composition and molecular packing. After describing several experimental methods to characterize the Langmuir monolayers, we discuss selected results from the last five years where monolayers were made to interact with pharmaceutical drugs, emerging pollutants and other biologically-relevant molecules. The challenges to take the field forward are also commented upon.

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“…Tawfik et al [200] assessed the effect of combining a composite NP composed of MB and AuNPs for aPDI of cutaneous fungal infection in rabbits caused by one of the commonest organisms in onychomycosis that is Trichophyton mentagrophytes . PDT was initiated at day 3 to 5 after inoculation, for four sessions 48 h apart.…”

Section: Apdi For Treating Localized Infectionsmentioning

confidence: 99%

Advances in antimicrobial photodynamic inactivation at the nanoscale

2017

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The alarming worldwide increase in antibiotic resistance amongst microbial pathogens necessitates a search for new antimicrobial techniques, which will not be affected by, or indeed cause resistance themselves. Light-mediated photoinactivation is one such technique that takes advantage of the whole spectrum of light to destroy a broad spectrum of pathogens. Many of these photoinactivation techniques rely on the participation of a diverse range of nanoparticles and nanostructures that have dimensions very similar to the wavelength of light. Photodynamic inactivation relies on the photochemical production of singlet oxygen from photosensitizing dyes (type II pathway) that can benefit remarkably from formulation in nanoparticle-based drug delivery vehicles. Fullerenes are a closed-cage carbon allotrope nanoparticle with a high absorption coefficient and triplet yield. Their photochemistry is highly dependent on microenvironment, and can be type II in organic solvents and type I (hydroxyl radicals) in a biological milieu. Titanium dioxide nanoparticles act as a large band-gap semiconductor that can carry out photo-induced electron transfer under ultraviolet A light and can also produce reactive oxygen species that kill microbial cells. We discuss some recent studies in which quite remarkable potentiation of microbial killing (up to six logs) can be obtained by the addition of simple inorganic salts such as the non-toxic sodium/potassium iodide, bromide, nitrite, and even the toxic sodium azide. Interesting mechanistic insights were obtained to explain this increased killing.

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A study of the treatment of cutaneous fungal infection in animal model using photoactivated composite of methylene blue and gold nanoparticle

Cited by 23 publications

References 22 publications

Genetic Tolerance to Rose Bengal Photodynamic Therapy and Antifungal Clinical Application for Onychomycosis

Genetic Tolerance to Rose Bengal Photodynamic Therapy and Antifungal Clinical Application for Onychomycosis

Recent advances in the use of Langmuir monolayers as cell membrane models

Advances in antimicrobial photodynamic inactivation at the nanoscale

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