A study of the supramolecular assembly formed by cucurbit[7]uril and 4-cyanophenol

Yu, Zhi-Chao; Lü, Yun; Zhao, Jie; Dai, Jingjing; Chen, Guorong; Shan, Pei‐Hui; Redshaw, Carl; Tao, Zhu; Xiao, Xin

doi:10.1016/j.molstruc.2023.134969

Cited by 2 publications

(2 citation statements)

References 37 publications

(66 reference statements)

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“…These included 4-6, where various electrondonating substitutions were incorporated around the 4-nitrophenyl ring. The analogous 4-cyanophenoxy silyl ethers 7-9 were also prepared, as the cyanophenoxy moiety would be a poorer leaving group [14,15], yet still be detectable by UV-VIS spectroscopy [16,17]. In addition, substrate 10, incorporating the azo dye 4-(4-nitrophenylazo)phenol as the chromophore, was also investigated.…”

Section: Design and Synthesis Of Substratesmentioning

confidence: 99%

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Development of Improved Spectrophotometric Assays for Biocatalytic Silyl Ether Hydrolysis

Lu,

Egedeuzu,

Taylor

et al. 2024

Biomolecules

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Reported herein is the development of assays for the spectrophotometric quantification of biocatalytic silicon−oxygen bond hydrolysis. Central to these assays are a series of chromogenic substrates that release highly absorbing phenoxy anions upon cleavage of the sessile bond. These substrates were tested with silicatein, an enzyme from a marine sponge that is known to catalyse the hydrolysis and condensation of silyl ethers. It was found that, of the substrates tested, tert-butyldimethyl(2-methyl-4-nitrophenoxy)silane provided the best assay performance, as evidenced by the highest ratio of enzyme catalysed reaction rate compared with the background (uncatalysed) reaction. These substrates were also found to be suitable for detailed enzyme kinetics measurements, as demonstrated by their use to determine the Michaelis−Menten kinetic parameters for silicatein.

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Section: Design and Synthesis Of Substratesmentioning

confidence: 99%

“…moiety would be a poorer leaving group [14,15], yet still be detectable by UV-VIS spectroscopy [16,17]. In addition, substrate 10, incorporating the azo dye 4-(4-nitrophenylazo)phenol as the chromophore, was also investigated.…”

Section: Ph Optimisation Of Assaymentioning

confidence: 99%