A Study of the Prolonged Intake of Small Amounts of Ethylenediaminetetraacetate on the Utilization of Low Dietary Levels of Calcium and Iron by the Rat

Abstract

Young rats were maintained for 84 days on a diet which supplied to each 0.15 mg of iron and 4.26 mg of calcium per day. The Ca:P ratio of the diet was 1.4. For some of the animals the dihydrate of disodium ethylenediaminetetraacetic acid (EDTA) was added at a level of 0.01%, which supplied 1 mg per rat per day. The molar ratio of EDTA:Ca:Fe was 1:37.9:1.The EDTA did not affect the growth of the rats. It did not affect the excretion of calcium, or its concentration in blood serum or femur. Since the faecal excretion was not affected, apparently the absorption was not impaired. The growth of the femur was not affected. EDTA was associated with generally higher levels of iron in the serum, but the average difference was not statistically significant. There was no effect upon the liver iron or the haemoglobin level in the blood.Small amounts of EDTA in the diet apparently did not interfere with the utilization of low and probably inadequate dietary levels of calcium and iron.

Algal Polysaccharides, Novel Applications and Outlook

“…Among the carrageenan types, carrageenan (primarely from C. crispus) has approximately twice the activity of unfractioned carrageenan and four times the activity of -carrageenan (Eucheuma cottoni and E. spinosum). The most active carrageenan has approximately onefifteenth the activity of heparin (Hawkins et al 1962), but the sulphated galactan from Grateloupa indica collected from Indian waters, exhibited anti-coagulant activity as potent as heparin (Sen et al 1994). The principal basis of the anti-coagulant activity of carrageenan appeared to be an anti-thrombotic property.…”

“…However, carrageenan inhibition of herpes simplex virus and HIV-1 infectivity were demonstrated as about a thousand-fold higher than the IC50s observed for genital HPVs in vitro (Witvrouw and de Clerck 1997;Luescher-Mattli et al 2003). Many reports exist of anti-coagulant activity and inhibited platelet aggregation of carrageenan (Hawkins et al 1962;Hawkins and Leonard 1963;Kindness et al 1979). Among the carrageenan types, carrageenan (primarely from C. crispus) has approximately twice the activity of unfractioned carrageenan and four times the activity of -carrageenan (Eucheuma cottoni and E. spinosum).…”

The Untapped Potential: Understanding Algal Sulphated Polysaccharides and their Applications: A review

“…Carrageenans also exhibit anticoagulant activity and inhibit platelet aggregation [129][130][131]. However, in comparison to heparin, the most active carrageenan has approximately one fifteenth activity [129]. Later on Sen et al [132] isolated a sulfated galactan from Grateloupa indica, exhibited anticoagulant activity as significant as heparin [132].…”