A study of the oxidative processes in human plasma by time-resolved fluorescence spectroscopy

Abstract

The aim of this study was to examine the usefulness of time-resolved fluorescence spectroscopy in the evaluation of the oxidative processes in human plasma. To investigate the impact of oxidative stress on the fluorescence of plasma, five studied markers (thiobarbituric acid-reactive substances, ischemia modified albumin, carbonyl groups, hydrogen peroxide, advanced oxidation protein products) were chosen as oxidative damage approved markers. Our method presents several advantages over traditional methods as it is a direct, non-time-consuming, repeatable, and non-invasive technique that requires only simple pre-treatment of samples without additional reagents and the sample size needed for analysis is small. In principle, each modification of the protein in plasma can be expected to modify its fluorescence properties and hence its lifetime or intensity. The study involved 59 blood donors with no evidence of disease. The research was conducted at excitation wavelengths of 280 nm and 360 nm, and emission was measured at wavelengths of 350 nm and 440 nm, respectively. Our results, although preliminary, suggest that the application of fluorescence measurements can be considered as an effective marker of oxidative stress. Regression analyses showed that a notable growth in fluorescence intensity at 440 nm and a simultaneous decrease in fluorescence intensity and mean fluorescence lifetime at 350 nm are associated with higher levels of oxidative stress.

Targeting the redox system for cardiovascular regeneration in aging

“…It is traditionally well characterized that excess H 2 O 2 , both endogenously and exogenously, promotes cellular senescence, which consequently develop CVD in aged organisms (Hu et al., 2022 ). Aged humans display elevated markers of oxidative stress in their plasma as opposed to young individuals (Wybranowski et al., 2022 ). During the past two decades, in the context of cardiovascular aging, it is increasingly unraveled that H 2 O 2 drives cellular senescence in both circulating and tissue‐resident EPCs and CPCs in aging microenvironment and impairs their function, compromising their regenerative capacity (Han & Kim, 2023 ; Imanishi et al., 2008 ).…”

Evidence of Hyperglycemic Levels Improving the Binding Capacity between Human Serum Albumin and the Antihypertensive Drug Hydrochlorothiazide

“…Under the experimental in vitro conditions used in this work, we did not induce the glycoxidation of albumin. We did not identify any significant change in the τ values for HSA and HSA/glucose that could indicate glycoxidation [44,45].…”

Employing Visible Light To Construct Photoinitiated Polymerized Nanofiltration Membranes for High Permeation and Environmental Micropollutant Removal

“…The time-resolved fluorescence lifetimes of RF in different systems were revealed to verify the cooperation between RF and TEA molecules, as shown in Figure d–f. Obviously, after exposure to TEA, the lifetime of 3 RF* was substantially shortened, indicating the successful transfer of electrons and H + between the photoinitiator and coinitiator …”

“…Obviously, after exposure to TEA, the lifetime of 3 RF* was substantially shortened, indicating the successful transfer of electrons and H + between the photoinitiator and coinitiator. 34 Membrane Formation Mechanism. DFT theoretical simulations derived from QC methods were implemented to explore the electron structures of the reactive monomers and the active formation sites of the crosslinking network in the photopolymerized PA membranes.…”