A study of the molluscicidal and larvicidal activities ofCitrullus colocynthis(L.) leaf extract and its main cucurbitacins against the molluscGalba truncatula, intermediate host ofFasciola hepatica

Chawech, Rachid; Njeh, Fatma; Hamed, Néjia; Damak, Mohamed; Àyadi, A.; Hammami, Hayet; Mezghani-Jarraya, Raoudha

doi:10.1002/ps.4479

Cited by 4 publications

(3 citation statements)

References 24 publications

(21 reference statements)

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“…Thus, this study was aimed at exploring the anti-cancer properties of extracts from Citrullus colocynth plant of the cucurbitaceae family that has been used as a traditional medicine to control diabetes ( Zaini et al, 2011 ; Shi et al, 2014 ). Reports about the nutritional and medicinal values of this plant and presence of many bioactive molecules had initially attracted our attention ( Sawaya et al, 1983 , 1986 ; Asyaz et al, 2010 ; Marzouk et al, 2010 ; Hussain et al, 2013 , 2014 ; Jeon and Lee, 2014 ; Chawech et al, 2017 ). Moreover, Tannin-Spitz et al (2007) found that an isolate (cucurbitacin glucosides) extracted from the leaves of this plant exhibits apoptotic activity in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Citrullus colocynth (L.) is a valuable cucurbit plant, widely distributed in the desert areas of the world, including India, known to possess nutritional values and diverse medicinal activities, including antibacterial, antifungal, larvicidal and anti-inflammatory properties ( Sawaya et al, 1986 ; Marzouk et al, 2010 ; Chawech et al, 2017 ). Literature documents the presence of many bioactive compounds, such as cucurbitacin, phenolic acids, flavonoids, pyridine and quinolone type alkaloids and fatty acids in fruits of these herbal plants ( Hussain et al, 2013 , 2014 ; Jeon and Lee, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Colocynth Extracts Prevent Epithelial to Mesenchymal Transition and Stemness of Breast Cancer Cells

et al. 2017

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Modern treatment strategies provide better overall survival in cancer patients, primarily by controlling tumor growth. However, off-target and systemic toxicity, tumor recurrence, and resistance to therapy are still inadvertent hurdles in current treatment regimens. Similarly, metastasis is another deadly threat to patients suffering from cancer. This has created an urgent demand to come up with new drugs having anti-metastatic potential and minimum side effects. Thus, this study was aimed at exploring the anti-proliferative and anti-metastatic potential of colocynth medicinal plant. Results from MTT assay, morphological visualization of cells and scratch assay indicated a role of ethanol and acetone extracts of fruit pulp of the colocynth plant in inhibiting cell viability, enhancing cell cytotoxicity and preventing cell migration in various cancer cell types, including breast cancer cell lines MCF-7 and MDA-MB-231, and cervical cancer cell line SiHa, subsequently having a low cytotoxic effect on mononuclear PBMC and macrophage J774A cells. Our study in metastatic MDA-MB-231 cells showed that both ethanol and acetone pulp extracts decreased transcript levels of the anti-apoptotic genes BCL2 and BCLXL, and a reverse effect was observed for the pro-apoptotic genes BAX and caspase 3. Additionally, enhanced caspase 3 activity and downregulated BCL2 protein were seen, indicating a role of these extracts in inducing apoptotic activity. Moreover, MDA-MB-231 cells treated with both these extracts demonstrated up-regulation of the epithelial gene keratin 19 and down-regulation of the mesenchymal genes, vimentin, N-cadherin, Zeb1 and Zeb2 compared to control, suggesting a suppressive impact of these extracts in epithelial to mesenchymal transition (EMT). In addition, these extracts inhibited colony and sphere formation with simultaneous reduction in the transcript level of the stemness associated genes, BMI-1 and CD44. It was also found that both the plant extracts exhibited synergistic potential with the chemotherapeutic drug doxorubicin to inhibit cancer viability. Furthermore, GC-MS/MS analysis revealed the presence of certain novel compounds in both the extracts that are responsible for the anti-cancer role of the extracts. Overall, the results of this report suggest, for the first time, that colocynth fruit pulp extracts may block the proliferative as well as metastatic activity of breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colocynth Extracts Prevent Epithelial to Mesenchymal Transition and Stemness of Breast Cancer Cells

et al. 2017

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“…Only a few studies have been conducted to test natural molluscicides [54,55]. A recent study on a new biomolluscicide, Citrullus colocynthis, shows that it kills the snail vector of fasciolosis, G. truncatula and also the larvae of F. hepatica without affecting the associated fauna of the snail [56]. Another solution proposed is the use of biological control.…”

Section: Which Solutions Can Limit Intermediate Host Proliferation Inmentioning

confidence: 99%

Impact of Human Activities on Fasciolosis Transmission

Sabourin

Alda

Vázquez

et al. 2018

Trends in Parasitology

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Fasciolosis is a worldwide disease caused by the liver fluke Fasciola spp. This food- and water-borne disease is a major public health and veterinary issue. It is currently (re)emerging in several regions mainly due to the rapid evolution of human activities. This article reviews the current knowledge of the impact of irrigation-system management, livestock management, and human diet and hygiene habits on the emergence of fasciolosis. We also identify the gaps in this knowledge and the possible solutions for limiting these impacts. Integrated control seems to be the most effective solution for controlling fasciolosis, because it enables monitoring, prevention, and rapid action in case of the (re)emergence of the disease.

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Control of Fasciolosis-Transmitting Lymnaeids in the Field

Rondelaud,

Vignoles,

Dreyfuss

et al. 2023

The Lymnaeidae

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A study of the molluscicidal and larvicidal activities ofCitrullus colocynthis(L.) leaf extract and its main cucurbitacins against the molluscGalba truncatula, intermediate host ofFasciola hepatica

Abstract: The ethyl acetate extract and both pure compounds proved the highest larvicidal activities, with a deterioration rate exceeding 89.2% (89.2-100%) and with no toxic effects against associated fauna. © 2016 Society of Chemical Industry.

Cited by 4 publications

References 24 publications

Colocynth Extracts Prevent Epithelial to Mesenchymal Transition and Stemness of Breast Cancer Cells

Colocynth Extracts Prevent Epithelial to Mesenchymal Transition and Stemness of Breast Cancer Cells

Impact of Human Activities on Fasciolosis Transmission

Control of Fasciolosis-Transmitting Lymnaeids in the Field

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