A study of the metabolism of phosphorus in mammalian red cells

Prankerd, T. A. J.; Altman, Kurt I.

doi:10.1042/bj0580622

Cited by 158 publications

(59 citation statements)

References 20 publications

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“…Prankerd and Altman (1954) observed that adenosine was more effective than glucose as a substrate for glyceraldehyde-3-phosphate formation in human erythrocytes. They suggested this was because adenosine had an ATPsparing effect and provided purine for ATP synthesis.…”

Section: Discussionmentioning

confidence: 97%

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

1982

Aust J Exp Biol Med

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Summary.During in vitro incubation in Krebs Ringer phosphate (KRP) medium, rat erythrocytes about 20% parasitized with B. rodhaini used two to four times as much gtucose as unparasitized erythrocytes. However, the ATP concentration and ATP production of parasitized erythrocytes decreased. For example, before incubation the ATP concentration in parasitized erythrocytes was significantly higher than unparasitized erythrocytes, but following 0-5 h incubation it was reduced to about the same as in unparasitized erythrocytes.Addition of 5 X 10-3 M adenosine to the basal medium increased the ATP production and the adenylate energy charge of parasitized erythrocytes. Infectivity tests in susceptible mice showed that, despite these seemingly favourable changes in energy metabolism, adenosine had no detectable effect on the survival of B. rodhaini. Absence of infectivity showed the inability of KRP medium to support B. rodhaini for 22 h in vitro with or without adenosine.Adenosine also greatly decreased the glucose uptake by parasitized erythrocytes, apparently by competitive inhibition, since lactate production was unchanged. INTRODUCTIONStudies of the metabolism of haemoprotozoan parasites of the genus Babesia are relevant to the production of living vaccines against bovine infections (Callow, 1977). The present series of investigations was designed to study the in vitro metabolism and survival in basal salts media of Babesia rodhaini.Metabolic studies of Babesia rodhaini (Rickard, 1969;Momen, 1979) provided information on the in vitro metabolism, particularly carbohydrate metabolism, of the parasite in basal salts media. There was no information, however, on the in vitro survival or energy metabolism of the parasite in basal media.

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Section: Discussionmentioning

confidence: 97%

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

1982

Aust J Exp Biol Med

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“…Phosphate esters were separated and analysed by methods described by Prankerd and Altman (20), and their specific activities estimated. No difference was found between normal and digoxin-treated (5 ug./ml.)…”

Section: The Lack Of Influence Of Digoxin On Cell Phosphate Esters Anmentioning

confidence: 99%

Diffusion and Permeation of Cations in Human and Dog Erythrocytes

Harris

Prankerd

1957

The Journal of General Physiology

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ABSTRAff£The kinetics of movement of tracer Na into human and dog red cells have been studied. The time courses of these processes and of K transfer were compared with the theoretical time course for saturation of a flat sheet having a resistive surface. The theoretical and the experimental curves when plotted againt t ~ have a considerable portion which is linear; on the basis of this resemblance the results are interpreted in terms of a permeability constant and an internal diffusion constant. It is supposed that selective adsorption acts to bring about concentration of K in the human cell and that the bulk of the Na of that cell is present in a thin outer region, while the K is in the interior. The action of stropha~athin is to remove the usual fimit to the Na capacity of the cell and it is proposed that the Na region increases in thickness at the expense of the K region. Omission of K from the medium has a similar result. Ha uptake into poisoned cells measured either with tracer or as a net gain has a linear dependence upon t½ after a delay. The permeability of the dog cell to Na is reduced when K is added to the medium; this may be due to the formation of an outer K-rlch region which imposes a resistance to Na movement.The movement of K into and from muscle has recently been shown to conform kinetic.ally with the idea that the ion is impeded by slow intraceltular diffusion as well as by the resistance offered by the cell membrane (Harris (10)).The purpose of the present paper is to examine data for cation movement into the erythrocytes of two species (man and dog) to see whether a combination of slow internal diffusion with surface resistance may not provide a better interpretation of the results obtained than a single permeability barrier. Most previous works on the cation movements have been analysed in terms of membrane permeability; Ponder (19), however, has criticised this neglect of the interior, which in the red cell shows evidence of being an orderly structure. When the resistance to movement through a membrane is so high that there is time for nearly complete diffusion mixing of the cell contents at all times, then uptake or loss will follow a first order law; that is, a rising or falling exponential function of time. More detailed investigation with tracers has, however, revealed that the cations of human and some other cells behave as if they were 197

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“…Some investigators have reported changes in red cell membrane permeability to Na+ and K+ following cholinesterase inhibition (Greig, Faulkner & Mayberry, 1953;Holland & Greig, 1950;Ludwig, Greig & Peterson, 1951) and in addition, considerable evidence has been presented indicating that the permeability of the cell membrane is dependent on cell metabolism (Bartlett & Marlow, 1953;Danowski, 1941;Dawson & Widdas, 1963;Gabrio, Finch & Huennekens, 1956 ; Harris, 1941 ;Jacob & Jandl, 1962b ; Prankerd, 1955 ;Prankerd & Altman, 1954). In view of these relationships, it was of interest to investigate the possibility of an alteration of cell metabolism by an anticholinesterase compound.…”

Section: Introductionmentioning

confidence: 99%

Effect of paraoxon on erythrocyte metabolism as measured by oxygen uptake in vitro

Santolucito

Whitcomb

1971

British J Pharmacology

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Summary1. Oxygen consumption in vitro and persistence in the general circulation of rabbit erythrocytes treated with the cholinesterase inhibitor paraoxon were determined. 2. Paraoxon in vitro reduced oxygen consumption below a measureable level within 2 hours. By contrast, the metabolic inhibitor N-ethylmaleimide (NEM) produced complete inhibition within 15 minutes. 3. Erythrocytes from rabbits orally dosed with parathion also exhibited marked depression of oxygen consumption. 4. Glutathione (GSH) restored oxygen uptake to pretreatment levels within 15 min in erythrocytes previously inhibited with NEM or paraoxon. 5. Erythrocytes treated with NEM were rapidly removed from the general circulation while paraoxon treated cells were removed at a rate comparable to untreated cells.

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A study of the metabolism of phosphorus in mammalian red cells

Cited by 158 publications

References 20 publications

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

Diffusion and Permeation of Cations in Human and Dog Erythrocytes

Effect of paraoxon on erythrocyte metabolism as measured by oxygen uptake in vitro

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