A study of the interactions between ephedrine and human serum albumin based on spectroscopic, electrochemical and docking assessments

Hashemnia, Sedigheh; Fard, Fatemeh Khajavi; Mokhtari, Zaynab

doi:10.1016/j.molliq.2021.118058

Cited by 12 publications

(8 citation statements)

References 42 publications

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“…The linear dependence of log( F 0 / F )/ F on log [Q] is seen in Figure 8 a. The slope was used to calculate the number of binding sites ( n ) for the molecule, while its intercept (log K a ) provided information on the binding constant [ 64 , 65 , 66 , 67 , 68 , 69 ].

…”

Section: Resultsmentioning

confidence: 99%

Hydroxychloroquine-Loaded Chitosan Nanoparticles Induce Anticancer Activity in A549 Lung Cancer Cells: Design, BSA Binding, Molecular Docking, Mechanistic, and Biological Evaluation

Elshami,

Shereef,

El-Mehasseb

et al. 2023

IJMS

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The current study describes the encapsulation of hydroxychloroquine, widely used in traditional medicine due to its diverse pharmacological and medicinal uses, in chitosan nanoparticles (CNPs). This work aims to combine the HCQ drug with CS NPs to generate a novel nanocomposite with improved characteristics and bioavailability. HCQ@CS NPs are roughly shaped like roadways and have a smooth surface with an average size of 159.3 ± 7.1 nm, a PDI of 0.224 ± 0.101, and a zeta potential of +46.6 ± 0.8 mV. To aid in the development of pharmaceutical systems for use in cancer therapy, the binding mechanism and affinity of the interaction between HCQ and HCQ@CS NPs and BSA were examined using stopped-flow and other spectroscopic approaches, supplemented by molecular docking analysis. HCQ and HCQ@CS NPs binding with BSA is driven by a ground-state complex formation that may be accompanied by a non-radiative energy transfer process, and binding constants indicate that HCQ@CS NPs–BSA was more stable than HCQ–BSA. The stopped-flow analysis demonstrated that, in addition to increasing BSA affinity, the nanoformulation HCQ@CS NPS changes the binding process and may open new routes for interaction. Docking experiments verified the development of the HCQ–BSA complex, with HCQ binding to site I on the BSA structure, primarily with the amino acids, Thr 578, Gln 579, Gln 525, Tyr 400, and Asn 404. Furthermore, the nanoformulation HCQ@CS NPS not only increased cytotoxicity against the A549 lung cancer cell line (IC50 = 28.57 ± 1.72 μg/mL) compared to HCQ (102.21 ± 0.67 μg/mL), but also exhibited higher antibacterial activity against both Gram-positive and Gram-negative bacteria when compared to HCQ and chloramphenicol, which is in agreement with the binding constants. The nanoformulation developed in this study may offer a viable therapy option for A549 lung cancer.

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…”

Section: Resultsmentioning

confidence: 99%

Hydroxychloroquine-Loaded Chitosan Nanoparticles Induce Anticancer Activity in A549 Lung Cancer Cells: Design, BSA Binding, Molecular Docking, Mechanistic, and Biological Evaluation

Elshami,

Shereef,

El-Mehasseb

et al. 2023

IJMS

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“…25 However, the standard rate constant (K s ) decreased significantly from 710 s −1 to 244 s −1 after the addition of HSA to the DAC solution, indicating that the interaction between DAC and HSA reduces the diffusion rate of DAC to the electrode surface due to the decrease in the number of free DAC molecules in the solution and the formation of the DAC-HSA complex. 10 Determination of the binding constant between the DAC and HSA.-The binding between the anticancer molecule DAC and HSA has not yet been reported by any other analytical method. In this work, the rapid characterization of these interactions at a physiological pH (0.1 M PBS, pH 7.4) was studied.…”

Section: °′mentioning

confidence: 99%

“…7 In the last years, several papers have been published concerning the investigation of the interaction between drugs and HSA, due to the valuable information obtained concerning the understanding of drug behavior in the body which can impact on their effectiveness and potential side effects. 8 Several analytical methods have been applied for this purpose, such as spectroscopy, 9 molecular docking, 10 fluorometry 11 and voltammetry. 12,13 The electrochemical methods provide a valuable insight into understanding the mechanisms and factors that govern drug binding and release from HSA in a relatively inexpensive way due to their high sensitivity, selectivity and versatility.…”

mentioning

confidence: 99%

Insights into the Interaction of Dacarbazine and Human Serum Albumin from Electrochemical Probing

Bakırhan

Brahmi

Tahani

2023

J. Electrochem. Soc.

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The interaction between dacarbazine (DAC) and human serum albumin (HSA) was investigated under physiological conditions using electrochemical techniques, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The CV results demonstrated that the oxidation of DAC on a pyrolytic graphite electrode (PGE) surface was irreversible and controlled by an adsorption-diffusion process. The addition of HSA was found to decrease the peak potential of DAC without altering the electrochemical parameters, which is likely due to the formation of an electro-inactive complex between the drug and protein, as supported by DPV and EIS measurements. Using DPV, the binding constant and stoichiometry of the complex were calculated to be 2.16×104 mol-1 L and 1:1, respectively. The temperature effect revealed that DAC binds to HSA through hydrophobic forces. In addition, the PGE electrode was successfully used to determine DAC in from biological samples.

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“…Also, Leucine 198 (Leu198), Phe211, Tryptophan 214 (Trp214), Leu238, and Histidine 242 (His242) residues were responsible for the stability of the complex via hydrophobic interactions. Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy was used to investigate the conformational changes of HSA during the interaction of ephedrine and HSA 24 .…”

Section: Voltammetrymentioning

confidence: 99%

A review on analytical methods for estimation of Oxaprozin

Thangabalan¹,

Ramachandra²,

Meruva³

2022

World J. Bio. Pharm. Health Sci.

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Oxaprozin is a non-narcotic, non-steroidal, anti-inflammatory drug (NSAID) used to relieve the inflammation, Swelling, stiffness, and joint pain associated with osteoarthritis, and rheumatoid arthritis. Oxaprozin is used to treat rheumatoid arthritis Osteoarthritis, dysmenorrhea and to alleviate moderate pain. This paper explains about the various analytical methods for the estimation of the drug.

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A study of the interactions between ephedrine and human serum albumin based on spectroscopic, electrochemical and docking assessments

Cited by 12 publications

References 42 publications

Hydroxychloroquine-Loaded Chitosan Nanoparticles Induce Anticancer Activity in A549 Lung Cancer Cells: Design, BSA Binding, Molecular Docking, Mechanistic, and Biological Evaluation

Hydroxychloroquine-Loaded Chitosan Nanoparticles Induce Anticancer Activity in A549 Lung Cancer Cells: Design, BSA Binding, Molecular Docking, Mechanistic, and Biological Evaluation

Insights into the Interaction of Dacarbazine and Human Serum Albumin from Electrochemical Probing

A review on analytical methods for estimation of Oxaprozin

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