A study of the interaction between fluorescein sodium salt and bovine serum albumin by steady-state fluorescence

Barbero, Nadia; Barni, Ermanno; Barolo, Claudia; Quagliotto, Pierluigi; Viscardi, G.; Napione, Lucia; Pavan, Simona; Bussolino, Federico

doi:10.1016/j.dyepig.2008.08.006

Cited by 141 publications

(62 citation statements)

References 30 publications

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“…So in order to evaluate the nature of quenching, the modified Stern-Volmer relation (Eq. 7) was used to get the linear plot (Barbero et al 2009). …”

Section: Uv-visible Absorption Studiesmentioning

confidence: 99%

Synthesis of 5-Fluorouracil conjugated LaF3:Tb3+/PEG-COOH nanoparticles and its studies on the interaction with bovine serum albumin: spectroscopic approach

et al. 2015

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The luminescent lanthanide-doped nanoparticles have gathered considerable attention in many fields especially in biomedicine. In this work, the lanthanum fluoride-doped terbium nanoparticles (LaF 3 :Tb 3? NPs) via simple chemical precipitation method has been synthesized and functionalized with polyethylene glycol. The size and the shape of the nanoparticles are confirmed using X-ray diffraction and transmission electron microscopy. The conjugation of 5-Fluorouracil (5-FU) and thus synthesized nanoparticles (NPs) were confirmed using various spectroscopic methods such as UVVisible spectroscopy, fluorescence steady state, and excited state spectroscopy studies. The enhancement in fluorescence emission (k = 543 nm) of drug-conjugated nanoparticles confirms the Vander Waals force of attraction due to F-F bonding between the drug and the nanoparticles. Further, the effects of 5FU-NPs in carrier protein were investigated using bovine serum albumin as a protein model. The 5FU-LaF 3 :Tb 3? nanoparticles binding is illustrated with binding constant and number of binding sites. The structural change of bovine serum albumin has been studied using circular dichroism and Fourier transform infrared spectroscopy analysis. Graphical AbstractKeywords Lanthanum fluoride Á 5-Fluorouracil Á Serum albumin Á Fluorescence quenching Á Vander Waal force of attraction

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“…So in order to evaluate the nature of quenching, the modified Stern-Volmer relation (Eq. 7) was used to get the linear plot (Barbero et al 2009). …”

Section: Uv-visible Absorption Studiesmentioning

confidence: 99%

Synthesis of 5-Fluorouracil conjugated LaF3:Tb3+/PEG-COOH nanoparticles and its studies on the interaction with bovine serum albumin: spectroscopic approach

et al. 2015

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“…At the same time, to solve this problem correctly for viscous solutions, the influence of processes of dye coupling with biomolecules must be taken into account [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Spectral and fluorescent indication of the acid-base properties of biopolymer solutions

et al. 2011

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Methods of absorption and fluorescent spectroscopy and fluorescent chronoscopy are used to investigate aqueous fluorescein solutions with pH values increasing from 1.79 to 11.67. For each method, the calibration curves are drawn for the fraction of dianion fluorescein species, quantum fluorescence yield, and amplitude dianion contribution to the fluorescence intensity on the pH values of aqueous solutions. The calibration curves allow the pH values of aqueous fluorescein solutions with gelatin (pH = 6.4 ± 0.1, 6.6 ± 0.2, and 6.6 ± 0.1), starch (pH = 6.3 ± 0.1, 6.3 ± 0.2, and 6.4 ± 0.1), and acid-soluble chitosan additions (pH = 5.4 ± 0.1, 5.6 ± 02, and 5.8 ± 0.1) to be estimated by these three methods. The pH values of biopolymer solutions obtained by the potentiometer method for starch (6.51) and gelatin (6.69) and by the three spectral and fluorescent methods are close; they are significantly underestimated (3.85) for chitosan solutions.

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“…In 2008, Barbero et al 19 noted the fluorophore could quench the intrinsic fluorescence emission of bovine serum albumin. Glasgow 20 also demonstrated intracellular quenching of fluorescein in corneal epithelial cells in 2016.…”

Section: Discussionmentioning

confidence: 99%

Monocarboxylate Transporters Mediate Fluorescein Uptake in Corneal Epithelial Cells

Sun

Liou

Yeh

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To determine the presence of monocarboxylate transporter (MCT) in human and rabbit corneal epithelium and its role in transcellular fluorescein transportation in the cornea. METHODS.The presence of MCTs in human and rabbit corneal epithelium was determined by RT-PCR and immunohistochemistry. Intracellular fluorescein uptake experiment was performed using cultured human corneal epithelial cells (HCECs). The involvement of MCT in fluorescein uptake was determined by addition of MCT inhibitors to HCECs and acute dry eye model on New Zealand albino rabbits by spectrophotometry, corneal impression cytology, and external eye photographs. RESULTS.MCT-1 and MCT-4 were identified in both human and rabbit corneal epithelia. A longer treatment period and a lower pH value in culture medium increased fluorescein uptake in HCECs. Fluorescein uptake in HCECs was decreased following addition of MCT inhibitors in a concentration-dependent manner. Impression cytology under fluorescent microscopy showed intracellular fluorescein staining in the rabbit cornea with acute dry eye treatment that was decreased following topical treatment of MCT inhibitors. CONCLUSIONS. Fluorescein ingress in corneal epithelial cells is mediated by the MCT family.Further study of MCT-mediated transport on HCECs may potentially benefit differential diagnosis and contribute better understandings of ocular surface disorders.

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A study of the interaction between fluorescein sodium salt and bovine serum albumin by steady-state fluorescence

Cited by 141 publications

References 30 publications

Synthesis of 5-Fluorouracil conjugated LaF3:Tb3+/PEG-COOH nanoparticles and its studies on the interaction with bovine serum albumin: spectroscopic approach

Synthesis of 5-Fluorouracil conjugated LaF3:Tb3+/PEG-COOH nanoparticles and its studies on the interaction with bovine serum albumin: spectroscopic approach

Spectral and fluorescent indication of the acid-base properties of biopolymer solutions

Monocarboxylate Transporters Mediate Fluorescein Uptake in Corneal Epithelial Cells

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