A Study of the Fertility of a Drosophila melanogaster MS Strain with Impaired Transposition Control of the gypsy Mobile Element

Kukushkina, I. V.; Makhnovskii, Pavel A.; Нефедова, Л. Н.; Балакирева, Е. А.; Romanova, Nadya; Kuzmin, I. V.; Lavrenov, A. R.; Kim, A. I.

doi:10.1134/s0026893320030097

Cited by 1 publication

(8 citation statements)

References 51 publications

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“…Knockout of six Hsp70 genes significantly (2-fold) reduced climbing speed at both time points (Figure 2). This is consistent with the previous work on this fly strain (23).…”

Section: Resultssupporting

confidence: 94%

“…Therefore, the w 1118 males (Vienna Drosophila Resource Center, ID 60000) and Hsp70 – males (genotype w 1118 ; Df(3R)Hsp70A, Df(3R)Hsp70B , Bloomington Drosophila Stock Center, ID 8841) with a knockout of 6 genes ( Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, Hsp70Bbb, Hsp70Bc ) (20) were used in this study. The lack of expression of these genes was verified by analysis of their open reading frame coverage (RNA sequencing) in a previous study (23). Flies were kept at 22 °C, humidity 45–55%, with a 12-hour light-dark cycle, on a food medium containing 5% sucrose, 10% baker’s yeast, 5% semolina, 0.7% agar, and 0.1% propionic acid.…”

Section: Methodsmentioning

confidence: 79%

“…In w 1118 flies, climbing speed rapidly drops after three weeks of life (23, 27, 28); therefore, compar and 23-day-old flies were used to study the effect of Hsp70 gene knockout. Knockout of six Hsp70 genes significantly (2-fold) reduced climbing speed at both time points (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…The protocol was described previously (23). Namely, legs from 15 flies were homogenized in the chilled RLT buffer (Qiagen, Germany) with 1% beta-mercaptoethanol in a 1.5-ml tube using a polypropylene pestle and a drill and then incubated (55 °C, 10 min) with protein kinase K (Evrogen, Russia).…”

Section: Rna Sequencing and Data Processingmentioning

confidence: 99%

“…Data processing was performed, as described previously (23). Low-quality reads and adapter sequences were deleted (Timmomatic tool, v 0.36), and then the reads were aligned to the BDGP6.94 primary genome assembly.…”

Section: Rna Sequencing and Data Processingmentioning

confidence: 99%

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Knockout ofHsp70genes significantly affects locomotion speed and gene expression in leg skeletal muscles ofDrosophila melanogaster

Makhnovskii,

Kukushkina,

Kurochkina

et al. 2023

Preprint

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The functions of theHsp70genes were studied using a line ofD. melanogasterwith knockout of six these genes out of thirteen. Namely, the effect of knockout ofHsp70genes on negative geotaxis climbing (locomotor) speed and the ability to adapt to climbing training (1.5 h/day, 7 days/week, 3 weeks) were examined. Seven- and 23-day-oldHsp70–flies demonstrated a comparable reduction (2-fold) in locomotor speed and widespread changes in leg skeletal muscle transcriptome (RNA-seq), compared to thew1118flies. To identify the functions of genes related to decreased locomotor speed the overlapped differentially expressed genes at both time points were analyzed: the up-regulated genes encoded extracellular proteins, regulators of drug metabolism and antioxidant response, while down-regulated genes encoded regulators of carbohydrate metabolism and transmembrane proteins. Additionally, in theHsp70–flies, activation of transcription factors related to disruption of the fibril structure and heat shock response (Hsf) were predicted, using the position weight matrix approach. In the control flies, adaptation to regular exercise training was associated mainly with gene response to a single exercise, while the predicted transcription factors were related to stress/immune (Hsf, NF-kB, etc.) and early gene response. In contrast, theHsp70–flies demonstrated no adaptation to training, as well as significantly impaired gene response to a single exercise. In conclusion, the knockout ofHsp70genes not only reduced physical performance, but also disrupted adaptation to regular physical training, which is associated with changes in leg skeletal muscle transcriptome and impaired gene response to an exercise.New & NoteworthyKnockout of sixHsp70genes inD. melanogasterreduced locomotion (climbing) speed that is associated with genotype-specific differences in leg skeletal muscle gene expression.Disrupted adaptation of theHsp70–flies to regular exercise training is associated with impaired gene response to a single exercise.

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“…Knockout of six Hsp70 genes significantly (2-fold) reduced climbing speed at both time points (Figure 2). This is consistent with the previous work on this fly strain (23).…”

Section: Resultssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Rna Sequencing and Data Processingmentioning

confidence: 99%

Section: Rna Sequencing and Data Processingmentioning

confidence: 99%

See 3 more Smart Citations

Knockout ofHsp70genes significantly affects locomotion speed and gene expression in leg skeletal muscles ofDrosophila melanogaster

Makhnovskii,

Kukushkina,

Kurochkina

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

A Study of the Fertility of a Drosophila melanogaster MS Strain with Impaired Transposition Control of the gypsy Mobile Element

Cited by 1 publication

References 51 publications

Knockout ofHsp70genes significantly affects locomotion speed and gene expression in leg skeletal muscles ofDrosophila melanogaster

Knockout ofHsp70genes significantly affects locomotion speed and gene expression in leg skeletal muscles ofDrosophila melanogaster

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