A study of the efficacy of ultrasonic waves in removing biofilms

Nishikawa, Takeshi; Yoshida, Akihiro; Khanal, Amit; Habu, Manabu; Yoshioka, Izumi; Toyoshima, Kuniaki; Takehara, Tadamichi; Nishihara, Tatsuji; Tachibana, Katsuro; Tominaga, Kazuhiro

doi:10.1111/j.1741-2358.2009.00325.x

Cited by 32 publications

(22 citation statements)

References 23 publications

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“…Furthermore, multiple factors affect the activity of UTMDemediated HBD-3 against biofilms infections. First, the US parameters, including acoustic intensity, frequency, duty cycle, and duration, are significant [35]. A high power and long durations of exposure were sufficient to destroy the biofilms but these conditions may cause tissue damage [30].…”

Section: Discussionmentioning

confidence: 99%

Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Zhu

Fang

et al. 2015

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Zhu

Fang

et al. 2015

Journal of Surgical Research

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“…The level of shear stress is largely dependent on US parameters [27], and we can adjust it with an acoustic pressure between 100 Pa and 1000 Pa [28]. According to Williams and Pitt [8], there appears to be an intensity threshold (between 100 and 10 mW/cm 2 ) below which ultrasound may not enhance the bactericidal effect of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on PlanktonicEscherichia coli

Zhu

Cai

Shi

et al. 2014

BioMed Research International

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Previous research has found that low-intensity ultrasound enhanced the lethal effect of gentamicin on planktonic E. coli. We aimed to further investigate whether microbubble-mediated low-intensity ultrasound could further enhance the antimicrobial efficacy of gentamicin. The planktonic E. coli (ATCC 25922) was distributed to four different interventions: control (GCON), microbubble only (GMB), ultrasound only (GUS), and microbubble-mediated ultrasound (GMUS). Ultrasound was applied with 100 mW/cm2 (average intensity) and 46.5 KHz, which presented no bactericidal activity. After 12 h, plate counting was used to estimate the number of bacteria, and bacterial micromorphology was observed with transmission electron microscope. The results showed that the viable counts of E. coli in GMUS were decreased by 1.01 to 1.42 log10 CFU/mL compared with GUS (P < 0.01). The minimal inhibitory concentration (MIC) of gentamicin against E. coli was 1 μg/mL in the GMUS and GUS groups, lower than that in the GCON and GMB groups (2 μg/mL). Transmission electron microscopy (TEM) images exhibited more destruction and higher thickness of bacterial cell membranes in the GMUS than those in other groups. The reason might be the increased permeability of cell membranes for gentamicin caused by acoustic cavitation.

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“…25 Phull et al showed that ultrasound is suitable for water desinfection, whereas ultrasound at high frequencies seemed to be more effective with regards to bactericidal effects. 30 Nishikawa et al demonstrated that Staphylococcus mutans biofilm can be effectively removed by ultrasonic exposure 31 in an in vivo model, and Xu et al demonstrated that high-intensity focused ultrasound is able to destroy Pseudomonas aeruginosa biofilm. 32 In contrast to that, Ensing et al showed that ultrasound alone did not negatively significantly affect bacterial viability (Escherichia coli, Staphylococcus aureus, coagulase negative staphylococci, Pseudomonas aeruginosa), neither planktonically nor in a biofilm.…”

Section: Discussionmentioning

confidence: 99%

Low‐frequency sonication may alter surface topography of endoprosthetic components and damage articular cartilage without eradicating biofilms completely

et al. 2014

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Two-stage exchange arthroplasty is the current standard of care for arthroplasty-related infections. Reinfection rates up to 30% are reported, and there is significant morbidity for the patient. In cases of failure, arthrodesis or amputation may result. Ultrasonic treatment has the potential to eradicate biofilms and avoid two-stage exchange arthroplasty. Data in the specific context of arthroplasty infections is scant, and there is debate regarding optimal frequency and intensity of treatment. Surface topography alterations of the endoprosthetic components and damage to adjacent bone and cartilage have not been investigated. We found incomplete biofilm eradication and significant increase in surface roughness (maximum peak-to-valley height) of cobalt-chrome unicondylar knee components as well as reduction in articular cartilage thickness area from 10 retrieved femoral heads after low-frequency sonication treatment according to manufacturer-specified recommendations. Our data collectively suggest that sonication treatment for biofilm eradication in arthroplasty infections may not be effective and surface topography alterations may potentially reduce implant longevity.

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A study of the efficacy of ultrasonic waves in removing biofilms

Abstract: This study demonstrated that ultrasonic wave exposure in a non-contact mode effectively removed adherent biofilms composed of S. mutans in vitro.

Cited by 32 publications

References 23 publications

Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Ultrasound microbubbles enhance human β-defensin 3 against biofilms

Microbubble-Mediated Ultrasound Enhances the Lethal Effect of Gentamicin on PlanktonicEscherichia coli

Low‐frequency sonication may alter surface topography of endoprosthetic components and damage articular cartilage without eradicating biofilms completely

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