A Study of the Correlation between Bacterial Culture and Histological Examination in Children with Helicobacter pylori Gastritis

Galoș, Felicia; Năstase, Gabriela; Boboc, Cătălin; Coldea, Cristina; Anghel, Mălina; Orzan, Anca; Bălgrădean, Mihaela

doi:10.5772/intechopen.80257

Cited by 3 publications

(2 citation statements)

References 18 publications

(20 reference statements)

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“…The use of prior antibiotics also contributes to low sensitivity and false negative results. The low sensitivity of culture in our study is in accordance with previous studies 20 . Another study conducted by Cosgun Yasemin et al showed that culture had lower sensitivity as compared to histopathol-ogy 11 .…”

Section: Discussionsupporting

confidence: 93%

The Diagnostic Accuracy of H. Pylori Culture and Rapid Urease Test in the Diagnosis of H.PYLORI Infection Keeping Histopathology as the Gold Standard

Ali

Nadeem

Gul

et al. 2022

JMS

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Objective: The aim of this study was to determine the diagnostic accuracy of biopsy-based tests in the detection of Helicobacter Pylori infections keeping histopathology as a gold standard. Materials and Methods: This validation study was conducted from August 2018 to February 2019 at Khyber medical college, pathology department, Peshawar, and comprised biopsy samples of 87 patients obtained via endoscopy which was done at the gastrointestinal endoscopy department, Hayatabad medical complex (HMC). Patients with a history of prior antibiotics and PPIs were excluded from the study. Rapid urease test (RUT) and culture of gastroduodenal biopsies were validated against the histopathological examination of gastroduodenal mucosa. Results: Out of 87 patients, 57.47% (50/87) were male and 42.52% (37/87) were female patients with an age range of 18 -80 years and a mean age of 45 years. Endoscopic findings showed 64.36% (56/87) of patients with gastritis, duodenitis with 6.89% (6/87), peptic and duodenal ulcers with 22.98% (20/87), erosion with 4.59% (4/87) and adenocarcinoma of stomach with 2.29% (2/87). The sensitivity and specificity of culture were 45.45% and 90.7% respectively, while positive and negative predictive values were 62.5% and 83% respectively. The sensitivity and specificity of the rapid urease test (RUT) were 81.81% and 90% while positive and negative predictive values were 90% and 94% respectively. Conclusion: The sensitivity and specificity of the rapid urease test were 82 and 90% respectively, while that of culture was 45 and 91% respectively. Keys Words: Helicobacter Pylori, Gastritis, Rapid urease test, Adenocarcinoma

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Section: Discussionsupporting

confidence: 93%

The Diagnostic Accuracy of H. Pylori Culture and Rapid Urease Test in the Diagnosis of H.PYLORI Infection Keeping Histopathology as the Gold Standard

Ali

Nadeem

Gul

et al. 2022

JMS

View full text Add to dashboard Cite

show abstract

“…In addition, identification based on colony morphology and characteristics of this bacterium lacks specificity; therefore, they could be excluded from the analysis. Furthermore, H. pylori -positive culture was found to be dependent on the type of pathology involved, and the number of biopsies taken could also affect the sensitivity of the culture [ 18 ]. A single biopsy specimen from the antrum provides high sensitivity, but it is not sufficient for a reliable diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance of Helicobacter pylori in Patients with Peptic Ulcer

Tran

et al. 2022

Medicina

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Background and Objectives: To determine the antibiotic resistance rate of H. pylori among patients with peptic ulcer. Materials and Methods: A cross-sectional monocentric study was conducted from January to December 2021 among patients aged from 16 years with gastrointestinal symptoms and esophagogastroduodenoscopy. Gastric mucosa biopsies were collected at the edges of the ulcer or at lesion sites for H. pylori culture. Five antibiotics (amoxicillin (AMX), clarithromycin (CLR), metronidazole (MTZ), levofloxacin (LEV), and tetracycline (TET)) were selected for antibiotic susceptibility testing. Results: One hundred and twenty-five patients were included, and the sex ratio was 0.6. Their mean age was 47.3 ± 14.2 years. All of the participants had gastritis, and 24.0% had duodenitis. A total of 21.6% of patients had a duodenal ulcer, and 12.8% had an antral ulcer. A total of 40 specimens have grown in H. pylori culture. The proportion of resistance to AMX, CLR, MTZ, LEV, and TET was 27.5%, 50%, 67.5%, 35%, and 5%, respectively. The proportion of multidrug resistance was 22.5%. The proportion of double resistance to AMX + CLR was 20.0%, AMX + MTZ was 15.0%, AMX + LEV was 2.5%, CLR + MTZ was 32.5%, and TET + MTZ was 5.0%. Conclusions: Our research results show that the treatment with MTX-TET or LVX-AMOX has the highest sensitivity rate. Therefore, practitioners should refer to these regimes to eradicate H. pylori in patients with gastric and duodenal ulcers. The reports on H. pylori eradication from different geographic areas show heterogeneous results. Therefore, continuous monitoring of antibiotic resistance of H. pylori in each population is very important. Having evidence helps clinicians to treat patients most effectively, reduce treatment costs, and limit the rate of antibiotic resistance.

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Antibiotic Resistance and Therapeutic Efficacy of Helicobacter pylori Infection in Pediatric Patients—A Tertiary Center Experience

et al. 2023

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One of the most widespread bacterial infections worldwide, Helicobacter pylori is thought to affect almost half of the world’s population. Due to rising antibiotic resistance, treatment should be tailored according to antibiotic susceptibility testing (AST). This study aims to evaluate Helicobacter pylori antibiotic resistance and its therapeutic efficacy in children. We conducted a prospective, single-center study, that evaluated 68 children referred for upper gastrointestinal endoscopy (UGE) following chronic dyspeptic syndrome. Each patient underwent multiple biopsies to perform bacterial cultures with AST and histopathological examinations for the diagnosis. Patients without antibiotic resistance or negative cultures received a 10-day sequential therapy, while the others had the antibiotic regimen tailored based on AST. Fifty-nine patients with a positive biopsy-based diagnosis (24 males) were finally included. Bacterial cultures with AST were positive for 13 patients (22.03%) and the antibiotic resistance for clarithromycin was 15.38%. Fifty-seven patients were administered sequential therapy with an eradication rate of 94.73%. Clarithromycin-resistant patients were successfully treated with 10-day triple therapy of esomeprazole, amoxicillin, and metronidazole. Although bacterial cultures had a low positivity rate, sequential therapy had a successful eradication rate. Further studies are necessary to better assess Helicobacter pylori antibiotic resistance to provide tailored treatment and identify children that need closer monitoring.

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A Study of the Correlation between Bacterial Culture and Histological Examination in Children with Helicobacter pylori Gastritis

Cited by 3 publications

References 18 publications

The Diagnostic Accuracy of H. Pylori Culture and Rapid Urease Test in the Diagnosis of H.PYLORI Infection Keeping Histopathology as the Gold Standard

The Diagnostic Accuracy of H. Pylori Culture and Rapid Urease Test in the Diagnosis of H.PYLORI Infection Keeping Histopathology as the Gold Standard

Antibiotic Resistance of Helicobacter pylori in Patients with Peptic Ulcer

Antibiotic Resistance and Therapeutic Efficacy of Helicobacter pylori Infection in Pediatric Patients—A Tertiary Center Experience

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