A Study of the Contractility, Biochemistry and Morphology of an Isolated Preparation of Human Skeletal Muscle

Moulds, R. F. W.; Young, Archie; Jones, David A.; Edwards, R. H. T.

doi:10.1042/cs0520291

Cited by 26 publications

(27 citation statements)

References 19 publications

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“…As previously recommended, measurements were confined to muscle segments located 5-6 mm from each extremity in order to ensure electrophysiological viability [5] and to minimise sarcomere damages. In addition, the ranges and mean values for Po were similar to those previously reported in bundles with both extremities sectioned either in humans [4] or animals [5]. Lastly, the existing correlation between isometric strength/MTMCSA ratio and Po corrected for muscle bundle CSA, gives credence to the measurements in this study.…”

Section: Discussionsupporting

confidence: 70%

“…As in other studies [4,5], the in vitro contractile parameters had relatively large standard SDs. Such a variation is expected considering that, despite close attention to technical details (all biopsies were performed by the same surgeon and all the contractile studies were carried out by the same investigator), Po corrected for muscle bundle cross-sectional area and c) maximal isometric twitch tension (Pt)/Po ratio of vastus lateralis muscle bundles for chronic obstructive pulmonary disease (COPD) (n=16) and controls (n=9).…”

Section: Discussionmentioning

confidence: 69%

“…A protocol allowing the study of sectioned human muscle bundles was initially developed to compare the contracture responses of human muscles to different drugs in order to determine the susceptibility to developing malignant hyperthermia [20]. Subsequently, MOULDS et al [4] succeeded in measuring in vitro contractile properties of human muscle bundles with cut ends that were harvested during general anaesthesia. To the best of the authors9 knowledge, this study is the first showing that human muscle bundles allowing measurement of in vitro contractile properties can be obtained under local anaesthesia.…”

Section: Discussionmentioning

confidence: 99%

“…These difficulties can be overcome by assessing the in vitro contractile properties of muscle bundles obtained through a small open biopsy using electrical field stimulation [4][5][6]. Under such conditions, the maximum force produced is totally independent from motivational factors and the muscle cross-section involved can be estimated with great precision.…”

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confidence: 99%

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In vitroandin vivocontractile properties of the vastus lateralis muscle in males with COPD

Debigaré

Ch²,

Fs³

et al. 2003

Eur Respir J

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Peripheral muscle weakness is common in chronic obstructive pulmonary disease (COPD) but it is still under debate whether weakness is due to atrophy or contractile dysfunction.In vitro and in vivo contractile properties of the vastus lateralis muscle were studied in 16 patients with stable COPD (forced expiratory volume in one second 39 ¡ 16% of predicted, age 67 ¡ 4 yrs (mean ¡ SD)) and nine sedentary control subjects. Isometric knee extensor strength was measured while mid-thigh muscle cross-sectional area (MTMCSA) was obtained using computed tomography. Muscle strips from the vastus lateralis obtained through open biopsy were rapidly suspended in an oxygenated KrebsRinger solution that was maintained at 35uC with a pH of 7.40 to study their contractile properties.The isometric knee extensors strength/MTMCSA ratio was 0.50¡0.08 versus 0.58¡0.06 kg?cm -2 for COPD and control subjects, respectively. The muscle bundle cross-sectional area (CSA) was 4.6¡2.1 and 4.4¡3.1 mm -2 , the length at which active tension was maximum was 15¡4 and 15¡3 mm, and maximal isometric peak forces normalised for CSA were 4.3¡2.7 and 4.8¡2.6 N?cm -2 for COPD and control subjects, respectively. The force/frequency relationship tended to be shifted to the right in patients with COPD, meaning that a higher stimulation frequency was necessary to produce the same relative force. Patients with COPD had a lower proportion of type I fibre than controls (26¡12% versus 39¡11%) with reciprocal significant increase in type IIb fibre proportion (20 ¡ 16% versus 8 ¡ 4%). The proportion of type IIa fibres was similar between the two groups.These results suggest that the contractile properties of the vastus lateralis are preserved in patients with chronic obstructive pulmonary disease. Therefore, the reduction in the quadriceps strength in patients with chronic obstructive pulmonary disease cannot be explained on the basis of an alteration of the contractile apparatus.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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In vitroandin vivocontractile properties of the vastus lateralis muscle in males with COPD

Debigaré

Ch²,

Fs³

et al. 2003

Eur Respir J

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“…In the open biopsy technique, large muscle samples (@2±3 cm in length, 0.5 cm 2 in cross-section) can be obtained and are often clamped prior to excision, thus helping to prevent hypercontraction (Bossen 2000;Moulds et al 1977;Schmalbruch 1975). Hypercontracted ®bers have either not been observed (Meltzer et al 1976;Moulds et al 1977;Schmalbruch 1975), or have been observed only rarely in healthy muscle samples obtained by open or surgical biopsy (Schmalbruch 1973). In muscle samples obtained by open biopsy from healthy subjects, Moulds et al (1977) reported hypercontraction only at the cut ends of muscle ®bers.…”

Section: Possible Sources Of Muscle ®Ber Hypercontractionmentioning

confidence: 99%

Muscle biopsy and muscle fiber hypercontraction: a brief review

Roth

Martel

Rogers

2000

European Journal of Applied Physiology

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The percutaneous muscle biopsy technique is an important tool used in exercise and applied physiology to study human skeletal muscle structure, adaptation, and regeneration. One important use of this technique has been the assessment of ultrastructural muscle damage, especially in tissue samples obtained following strenuous exercise protocols, often involving eccentric muscle actions. In this brief review, we define and describe hypercontracted fibers, and outline how such fibers may adversely affect the interpretation of muscle damage from fixed tissue. Evidence suggests that hypercontracted fibers present in healthy skeletal muscle samples are likely to be artifacts related to the muscle biopsy procedure, as opposed to intrinsic degeneration present prior to the biopsy. When hypercontracted fibers are noted as being intrinsic to a muscle sample (e.g., in myopathy or following an extreme eccentric stimulus), such fibers are generally associated with the infiltration of inflammatory cells, indicative of a regenerative response. In contrast, hypercontracted fibers resulting from the biopsy procedure are not typically associated with an inflammatory response. The major sources of hypercontracted fibers are outlined and recommendations for their interpretation are discussed.

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Problems in the diagnosis of mitochondrial cytopathies

Salganicoff¹,

Selak²

1998

BioFactors

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A Study of the Contractility, Biochemistry and Morphology of an Isolated Preparation of Human Skeletal Muscle

Cited by 26 publications

References 19 publications

In vitroandin vivocontractile properties of the vastus lateralis muscle in males with COPD

In vitroandin vivocontractile properties of the vastus lateralis muscle in males with COPD

Muscle biopsy and muscle fiber hypercontraction: a brief review

Problems in the diagnosis of mitochondrial cytopathies

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