A study of the conditions of the supercritical fluid extraction in the analysis of selected anti-inflammatory drugs in plasma

Klimeš, Jiřı́; Sochor, J; Kříž, J.

doi:10.1016/s0014-827x(01)01182-x

Cited by 8 publications

(2 citation statements)

References 16 publications

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“…The detection of SAA in freeze dried serum dissolved in Milli-Q water, however, suggests more hydrophilic properties than previously believed, or suggests interactions between SAA and other serum substances with more hydrophilic properties. Hydrophilic properties of SAA or SAA complexes, could also explain the failure to extract SAA by SFE, as lipophilic and ampophilic/lipophilic substances are expected to be extractable by this procedure, as previously observed for different drugs [36]. Additional studies are needed to investigate whether the biochemical properties of equine SAA is of similar nature as the recently observed properties demonstrated in pigs [29], but unlike in porcine SAA purification [29], HIC has previously been shown to be useful as an initial step for purification of equine SAA [5].…”

Section: Discussionmentioning

confidence: 75%

Investigation of the solubility and the potentials for purification of serum amyloid A (SAA) from equine acute phase serum – a pilot study

et al. 2013

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BackgroundSerum amyloid A (SAA) is useful as a diagnostic marker of systemic inflammation in horses, but only heterologous assays based on non-equine calibration and standardization are available for measurements of equine SAA. More accurate measurements could be obtained using purified species-specific SAA in native conformation for assay calibration and standardization. Further knowledge about the biochemical properties of SAA would facilitate a future production of native species-specific calibration material Therefore, the aim of the study was an investigation of the solubility and potentials for purification of equine SAA based on biochemical properties.Freeze dried equine acute phase serum was dissolved in 70% 2-propanol, 8 M urea, and milli-Q water, respectively. Supercritical fluid extraction (SFE), size-exclusive chromatography (FPLC-SEC), and preparative isoelectric focusing (IEF) were performed in the attempt to purify. Immunostaining of IEF blots were used for isoform-specific detection of SAA in the preparations and purity was assessed by silverstained SDS-PAGE.FindingsSAA was soluble in 70% 2-propanol, 8 M urea and Milli-Q water. SAA was not separated in the lipophilic or ampipathic fractions following SFE. SAA was included in a FPLC-SEC-fraction of 237 kDa, despite the molecular weight known to be much smaller, suggesting binding to other serum constituents. SAA precipitated following separation of other serum proteins by preparative IEF.DiscussionNo effective purification of SAA was achieved in the present study, but findings important for future investigations were made. The study suggested that SAA is not exclusively hydrophobic, but appears less hydrophobic when interacting with other serum components. These results suggest more complex aspects of solubility than previously believed, and indicate potentials for purification of native SAA.

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Section: Discussionmentioning

confidence: 75%

Investigation of the solubility and the potentials for purification of serum amyloid A (SAA) from equine acute phase serum – a pilot study

et al. 2013

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“…Sc-carbon dioxide is reported to be used to extract ibuprofen, indomethacin and flufenamic acid from plasma sample and analysed using high performance liquid chromatography method. 70 Apart from this, benzodiazepines are also reported to be separated from the dosage form matrix using supercritical fluid technology. 71…”

Section: Drug Extraction and Analysismentioning

confidence: 99%

Supercritical Fluid Technology and its Pharmaceutical Applications: A Revisit with Two Decades of Progress

Bin¹,

Janakiraman²,

Razak³

et al. 2020

IJPER

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Over the past two decades, supercritical fluid technology has emerged as one of the most important technologies applied in many fields such as cosmetic, food and pharmaceutical. Supercritical fluid extraction process offers numerous advantages such as easy, effective, inexpensive, high quality of solute extraction and environmentally friendly. This mini-review describes the fundamentals of supercritical fluid technology, the function of supercritical fluid as solvent and anti-solvent, mechanism of supercritical fluid, advantages and disadvantages and revisit the application of supercritical fluid technology in pharmaceutical sciences. In-depth discussion with recent examples of extraction of natural products, particle design in drug delivery, preparation on pharmaceutical powder, drug solubilization, inclusion complex, polymer impregnation, liposomal formulations, purification and polymorphism as well as drug extraction analysis are also presented. Key aspects and processing considerations of supercritical fluid technologies are reviewed to assist scientists to generate other related experimental works.

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Appendix B References to Analytical Methods for Determination of Ibuprofen in Biological Fluids, Principally Plasma

Rainsford

2015

Ibuprofen

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A study of the conditions of the supercritical fluid extraction in the analysis of selected anti-inflammatory drugs in plasma

Cited by 8 publications

References 16 publications

Investigation of the solubility and the potentials for purification of serum amyloid A (SAA) from equine acute phase serum – a pilot study

Investigation of the solubility and the potentials for purification of serum amyloid A (SAA) from equine acute phase serum – a pilot study

Supercritical Fluid Technology and its Pharmaceutical Applications: A Revisit with Two Decades of Progress

Appendix B References to Analytical Methods for Determination of Ibuprofen in Biological Fluids, Principally Plasma

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