A study of the composition of Clun Forest ewe's milk. IV. The proteins of ewe's milk and their variation with stage of lactation

Poulton, S. G.; Ashton, W. M.

doi:10.1017/s0021859600016919

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…The higher fat contents and calorific values of twin-suckled ewes compared with single-suckled ewes, and the changes in the percentages of fat, protein and calorific values, followed similar trends to those reported by Gardner, Hogue & Bensadoun (1964). Poulton & Ashton (1970) recorded a steady increase in fat and protein after the second week of lactation. In the present study a similar trend was found in respect of protein, but the fat content did not increase until after the fifth week of lactation.…”

Section: Discussionsupporting

confidence: 81%

The yield and composition of the milk of Finnish Landrace × Blackface ewes: I. Ewes and lambs maintained indoors

1972

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Twenty-two Finnish Landrace x Blackface ewes suckling either single, twin, triplet or quadruplet lambs were maintained indoors during a 12-week lactation period. Milk production was estimated at weekly intervals by hand milking following oxytocin administration. Milk was collected from each ewe in four similar volumetric portions which were retained in order of withdrawal and analysed for maj or constituents. Weighted volumes of milk from each of the four portions were bulked to give a single sample for analysis. Estimated mean total milk production over a 12-week period from parturition was 134, 205, 212 and 218kg for single-, twin-,triplet-and quadruplet-suckled ewes respectively. Most of the difference in total yield between multiple-suckled groups of ewes occurred during the first 3 weeks of lactation, and the data indicate that the number of lambs born, or the total weight of concepta, influences the initial quantity of milk available to the lambs. Regression analysis showed significant evidence for difference between the linear components of the regression of milk yield, fat, protein, lactose and Gross Energy on the stage of lactation for all suckling groups. The concentration of all constituents other than lactose and ash was higher in colostral milk, but was significantly higher only in respect of fat, protein and Gross Energy. Substantial differences found between suckling groups in respect of fat and protein, content of the milks indicate an influence on these constituents due to the number of lambs suckled. The mean growth rates of the triplet-and quadruplet-suckled groups of lambs were each significantly lower than each of the single-and twin-suckled groups. The food intake of the ewes in relation to shape of lactation curves and consequent lamb growth is discussed. (1957) have shown that durunder similar conditions, Finnish Landrace x Black-ing the milking process, the fat content of cow's milk face ewes produce 70 % more lambs at birth than may rise from 1 to 2 % in the first drawn milk to pure-bred Blackface ewes. It is possible, therefore, 8-15 % in the last portion. In sheep, Rusev, that the genetic influence of the Finnish Landrace Georgiev & Tanev (1964) found that the fat content breed could be used to increase the number of lambs of the last 40 % of milk extracted may be double reared in the hill areas of Britain, particularly in that of the first 60 % withdrawn. It is probable, situations where the natural environment has been therefore, that some of the variation reported in improved. However, it is not known if this greater the constituent values of ewe's milk in the literature prolificacy is associated with an adequate capacity is due to differences in sampling technique, for milk production to sustain acceptable growth This paper is concerned with the yield and cornrates in litters of lambs. Donald, Read & Russell (1968) have shown that, Johansson & Claessonposition of ewe's milk and the variation in composiInformation from several sources, quoted by tion occurring at sequential stages ...

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Section: Discussionsupporting

confidence: 81%

The yield and composition of the milk of Finnish Landrace × Blackface ewes: I. Ewes and lambs maintained indoors

1972

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“…The quantity of milk produced as influenced by suckling intensity did not affect the percentage content of solids non-fat, lactose, or ash which is in agreement with the findings of Peart et al (1972). Poulton & Ashton (1970) recorded a steady increase in the percentage of fat and protein of ewe's milk after the second week of lactation. In the present study fat content declined after the first week of lactation and remained relatively constant until after the sixth week; a similar trend was obtained by Peart et al (1972).…”

Section: Acs 85supporting

confidence: 88%

“…The mean values of milk composition are within the range of published values (Barnicoat, Logan & Grant, 1949;Poulton & Ashton, 1970;Peart et al 1972). The mean fat content of the milks of groups of ewes suckling single, twin or triplet lambs was, respectively, 2-5, 1-6 and 1-0% greater than the values obtained by Peart et al (1972) for ewes indoors, and the percentage fat did not vary significantly with the number of lambs suckled.…”

Section: Acs 85supporting

confidence: 77%

The yield and composition of the milk of Finnish Landrace × Blackface ewes: II. Ewes and lambs grazed on pasture

Peart¹,

Edwards²,

Donaldson³

1975

J. Agric. Sci.

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The milk production of 14 Finnish Landrace x Blackface ewes suckling either single, twin or triplet lambs was recorded while they were wholly maintained on a high-quality grazed pasture. Intravenous administration of oxytocin followed by hand milking was used to estimate milk production within 4 days of parturition and then at weekly intervals during a 12-week lactation period. At each milking the milk from each ewe was retained for analysis. Herbage samples for analysis were cut by hand shears at weekly intervals at a height simulating the harvesting by the sheep. In vitro analysis showed a decline in the digestible organic matter in the herbage from approximately 75 % in the early stages, to around 67 % at the end of lactation. The percentage crude protein in the dry matter of the herbage was variable but tended to increase towards the end of the lactation period. The estimated mean total milk production values were 125, 176 and 193 kg for single-, twin-and triplet-suckled groups of ewes, respectively. Almost all of the difference in total yield between the groups of multiplesuckled ewes occurred in the first 4 weeks of lactation. Analysis of variance showed no significant differences between suckling groups in the concentration of any of the milk constituents. Regression analysis showed significant evidence for differences between the linear and quadratic components of the regressions on stage of lactation for the suckling groups only in respect of daily milk yield, energy and protein production. The concentration of constituents other than lactose was higher in colostral milk but the differences were statistically significant only in respect of energy, total solids and fat. The lactose content of ordinary milk was significantly higher than that of colostral milk. The mean daily live-weight gains of the single-and twin-suckled lambs were significantly greater than that of the triplet-suckled group.

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“…Those findings correspond to ovine milk protein composition during lactation. Concentrations of caseins, total albumins, and β-lactoglobulin in whole milk increased significantly over the course of lactation (Poulton and Ashton, 1970). No milk protein fractions were analyzed in the present study, but previous studies in dairy cows showed different composition during lactation by contrast with ewes.…”

Section: Milk Protein Gene Expressioncontrasting

confidence: 52%

Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

Sigl¹,

Meyer²,

Wiedemann³

2012

CZECH J ANIM SCI

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ABSTRACT:The objective of the present study was to refine a previously developed method to isolate primary bovine mammary epithelial cells (pBMEC) from fresh milk. Using this method, it was tested whether the number of pBMEC and the relation of recovered pBMEC to total somatic cell count vary within the individual lactation stages. Furthermore, the expression levels of the milk protein genes during the first twenty weeks of lactation were determined by quantitative PCR method. A total number of 152 morning milk samples were obtained from twenty-four Holstein-Friesian cows during the first 20 weeks of lactation (day 8, 15, 26, 43, 57, 113, and 141 postpartum). Numbers of extracted pBMEC were consistent at all time-points (1.1 ± 0.06 to 1.4 ± 0.03 ×10 3 /ml) and an average value of RNA integrity number (RIN) was 6.3 ± 0.3. Percentage of pBMEC in relation to total milk cells (2.0 ± 0.2 to 6.7 ± 1.0%) correlated with milk yield. Expression patterns of the casein genes alpha (α) S1 , (α) S2 , beta (β), and kappa (κ) (CSN1S1, CSN1S2, CSN2, CSN3, respectively) and the whey protein genes α-lactalbumin (LALBA) and progestagen-associated endometrial protein (PAEP; known as β-lactoglobulin) were shown to be comparable, i.e. transcripts of all six milk protein genes were found to peak during the first two weeks of lactation and to decline continuously towards mid lactation. However, mRNA levels were different among genes with CSN3 showing the highest and LALBA the lowest abundance. We hypothesized that milk protein gene expression has a pivotal effect on milk protein composition with no influence on milk protein concentration. This paper is the first to describe milk protein gene expression during lactation in pBMEC collected in milk. Future studies will be needed to understand molecular mechanisms in pBMEC including regulation of expression and translation throughout lactation.

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A study of the composition of Clun Forest ewe's milk. IV. The proteins of ewe's milk and their variation with stage of lactation

Cited by 8 publications

References 17 publications

The yield and composition of the milk of Finnish Landrace × Blackface ewes: I. Ewes and lambs maintained indoors

The yield and composition of the milk of Finnish Landrace × Blackface ewes: I. Ewes and lambs maintained indoors

The yield and composition of the milk of Finnish Landrace × Blackface ewes: II. Ewes and lambs grazed on pasture

Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

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