A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

Dustin, Isabelle; Vollenweider, Pierre; Hofer, Rose‐Marie; Hainard, Pierre

doi:10.1007/bf00202131

Cited by 5 publications

(8 citation statements)

References 26 publications

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“…The computer, the method used for image analysis and the statistical treatment of the variables are described in Dustin et al (1994).…”

Section: Methodsmentioning

confidence: 99%

“…The preparation artefacts are then corrected in both types of tissues. Finally, the corrected images are binarized and analysed, after removal of parenchyma and companion cells in the conductive phloem (Dustin et al 1994). …”

Section: R E S U L T Smentioning

confidence: 99%

“…Limits with bordering tissues are particularly well defined, because cell division and differentiation stopped at the end of the vegetative period. Zone cutting during image analysis is made easier by this feature (Dustin et al 1994).…”

Section: R E S U L T Smentioning

confidence: 99%

“…This study is divided into two parts: I reports the methods of sample preparation and the results obtained about the relationships between structure and age, and II describes the computer procedures (Dustin et al 1994). The cambial zone and conductive phloem of young and old trees were analysed from the stem, an easy to sample organ providing interesting opportunities for further work in plant physiology.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, quantification is a major difficulty in histology, yet without it the implications of such changes on tree vitality cannot be understood. Automatic image analysis provides quick and easy quantitative measurements of size and shape, and therefore constitutes a useful tool to study tissue and cell structure (Dustin et al 1994).…”

Section: Introductionmentioning

confidence: 99%

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A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

Vollenweider

Dustin

Hofer

et al. 1994

Trees

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Abstract. Quantification is a major problem when using histology to study the influence of ecological factors on tree structure. This paper presents a method to prepare and to analyse transverse sections of cambial zone and of conductive phloem in bark samples. The following paper (II) presents the automated measurement procedure. Part I here describes and discusses the preparation method, and the influence of tree age on the observed structure. Highly contrasted images of samples extracted at breast height during dormancy were analysed with an automatic image analyser. Between three young (38 years) and three old (147 years) trees, age-related differences were identified by size and shape parameters, at both cell and tissue levels. In the cambial zone, older trees had larger and more rectangular fusiform initials. In the phloem, sieve tubes were also larger, but their shape did not change and the area for sap conduction was similar in both categories. Nevertheless, alterations were limited, and demanded statistical analysis to be identified and ascertained. The physiological implications of the structural changes are discussed.

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“…The computer, the method used for image analysis and the statistical treatment of the variables are described in Dustin et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Section: R E S U L T Smentioning

confidence: 99%

Section: R E S U L T Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

Vollenweider

Dustin

Hofer

et al. 1994

Trees

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Within–tree Variation in Phloem Cell Dimensions and Proportions in Eucalyptus Globulus

Quilhó¹,

Pereira²,

Richter³

2000

IAWA J

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The axial variation of bark thickness and quantitative anatomical features of Eucalyptus globulus bark were analysed for one site based on individual measurements of ten 15-year-old trees at six height levels (DBH, 5%, 15%, 35%, 55% and 75% of total tree height). The parameters studied were: length, tangential diameter and percentage of sieve tubes; length, width, cell wall thickness and percentage of fibres; height and percentage of rays; percentage of sclereids in the secondary phloem. Bark thickness decreases from base to top of the tree. Fibre width and wall thickness decrease from base upwards. No distinct axial patterns of variation were observed for the other biometric variables studied. Parenchyma is the main cell type of the bark (50%) followed by fibres (27.9%), rays (12.1%), sieve tubes (2.7%), and sclereids (7.3%). The cell type proportions vary significantly within the tree, i.e., parenchyma, ray and sclereid proportions decrease, fibre and sieve tube proportions increase towards the top of the tree.

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Cellular and topochemical characteristics of secondary changes in bark tissues of beech (Fagus sylvatica)

Prislan¹,

Koch²,

Schmitt³

et al. 2012

Holzforschung

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Anatomical and histometrical investigations were carried out on bark tissues (non-collapsed and collapsed phloem, and periderm) of beech (Fagus sylvatica L.) trees growing at a forest site near Ljubljana, Slovenia (400 m a.s.l.). Secondary changes in bark, especially sclereid formation and lignification, were followed at cellular and sub-cellular levels by light microscopy (LM), UV microspectrophotometry (UMSP), and transmission electron microscopy (TEM). The average width of the whole bark was 5960 mm; non-collapsed phloem on average occupied 4%, collapsed phloem 89%, and periderm 7% of the bark. Secondary changes in mature phloem were characterized by a collapse of sieve tubes, inflation of axial parenchyma, and development of sclereids. The percentage of sclereid areas and the stage of their development elevated with increasing distance from the cambium. The most pronounced increment in the sclereid proportion was observed in the second-and third-fifth of the bark. Sclereid cell walls were thick and poly-lamellated and had similar spectral characteristics but distinctly higher UV absorbance values than xylem fibers. By means of a combination of LM, TEM and UMSP techniques, the structure and secondary changes in the bark could be precisely identified.

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A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

Cited by 5 publications

References 26 publications

A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

A study of the cambial zone and conductive phloem of common beech (Fagus sylvatica L.) using an image analysis method

Within–tree Variation in Phloem Cell Dimensions and Proportions in Eucalyptus Globulus

Cellular and topochemical characteristics of secondary changes in bark tissues of beech (Fagus sylvatica)

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