A study of the behaviour of some sixty amino-acids and other ninhydrin-reacting substances on phenol-‘collidine’ filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids

Dent, C. E.

doi:10.1042/bj0430169

Cited by 614 publications

(65 citation statements)

References 11 publications

(11 reference statements)

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“…stance in the patient's urine migrated to a slightly different position, indeed to a position marked on our reference map (Dent, 1948) as that occupied by homocysteic acid. Addition of homocysteic acid showed exact correspondence with the urine spot (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The chromatographic methods used in Belfast have been described by Carson and Neill (1962). Subsequent work in London was done by the technique of Dent (1948). All urine specimens from each of the affected sibs constantly gave a moderately strong cyanide nitroprusside test for cystine (Brand, Harris and Biloon, 1930) and a strong spot on the chromatograms of the oxidized urines in the region of cysteic acid (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The urine findings were confirmed, and polaro- graphic quantitative analyses showed, as expected, raised plasma 'cystine' levels and a normal renal clearance. Further study of the paper chromatograms, however, indicated that the 'cystine' spot was not in the exact position for cystine but in the nearby position occupied by homocystine (Dent, 1948). On the automatic amino acid analyser which had just been constructed, the results were even less ambiguous.…”

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confidence: 99%

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Homocystinuria: A New Inborn Error of Metabolism Associated With Mental Deficiency

1963

JAMA

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Homocystinuria: A New Inborn Error of Metabolism Associated With Mental Deficiency

1963

JAMA

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“…It takes a relatively large amount of histidine to obtain enough ninhydrin color for accurate measurement. Consequently, histidine may be better determined by other reagents (e.g., use of diazotized sulphanilic acid (3,8)). Leucine and isoleucine do not separate under the conditions described.…”

Section: Plant Physiologymentioning

confidence: 99%

“…(See Part I, refs. 7,8,9,12,13. ) Unless the selectivity of the "2-directional" procedures is exploited in the quantitative method, chromatographic methods would be inapplicable to most biological problems.…”

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confidence: 99%

Investigations on Nitrogen Compounds and Nitrogen Metabolism in Plants. Ii. Variables in Two-Directional Paper Chromatography of Nitrogen Compounds: A Quantitative Procedure

Thompson

Steward

1951

Plant Physiol.

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In Part I (21), the conditions under which the ninhydrin reaction on paper may be carried out quantitatively and applied to 2-directional paper chromatograms have been defined.In this part (II) of the investigation, the variables operating in the chromatography will be considered. It will be shown that when these variables are brought under control, the ninhydrin reaction on paper may be applied in such a way as to permit the quantitative determination of a large number of amino acids on a single chromatogram sheet.Hitherto, workers who have concerned themselves with quantitative paper chromatography of amino acids have concentrated mainly on the means to be adopted for determining the amino acids after chromatography, whether by ninhydrin or otherwise. (See Part I, refs. 5-14.) Also, most of the quantitative procedures hitherto described have utilized only the 1-directional, or "strip," chromatograms. (See Part I, refs. 7,8,9,12,13.) Unless the selectivity of the "2-directional" procedures is exploited in the quantitative method, chromatographic methods would be inapplicable to most biological problems. No strip or 1-directional method can readily reveal the great variety of amino compounds known to be present in plants or in protein hydrolysates.The objective was a method by which the amino acids could first be separated on paper and then quantitatively determined. This method requires not only clean-cut, but reproducible, separations on paper of as many amino compounds as possible, but also that the quantitative recovery should be reproducible and preferably complete to avoid the necessity of calibrating

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GLUTAMINE, GLUTAMIC ACID, AND Γ-Aminobutyric ACID IN CEREBROSPINAL FLUIDS

Ludewig¹

1953

Arch NeurPsych

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THE ROLES of glutamic acid and its derivatives glutamine and \g=g\-aminobutyric acid, which are present in nerve tissue in appreciable quantities, are poorly understood.1 It is possible that the concentrations of these compounds in cerebrospinal fluid may reflect changes in nerve tissue metabolism resulting from neurological disorders. Since available data are scanty,2 it seemed worth while to determine the concentrations of glutamic acid and related compounds in the cerebrospinal fluid in a group of patients with various types of neurological disorders. METHODS AND MATERIALSpecimens of cerebrospinal fluid were obtained from patients in the University of Virginia Hospital undergoing pneumoencephalograms for diagnosis. Each specimen was contrifuged for removal of cellular debris and, whenever possible, was analyzed immediately for glutamic acid and glutamine. It was determined that the values obtained were not changed after overnight storage at 5 C.Glutamic acid and glutamine were determined by the procedures recommended by Krebs,3 which utilize the enzymes in Clostridium welchii (per fringens) for deamination of glutamine and decarboxylation of glutamic acid. Cl. welchii (strain SR-12) was grown in a medium used by Meister, Sober, and Tice.4 The organisms were harvested and lyophilized, and 15 mg. of the dried material was used with 3.5 ml. of cerebrospinal fluid. The Warburg manometric pro¬ cedure was used for determining the carbon dioxide, and the Archibald 5 technique, utilizing vacuum distillation and Nesslerization, was employed for estimating ammonia. In order that the best performance of the distillation apparatus should be insured,8 it was found necessary to pass air continuously through the capillary to avoid clogging. All samples were analyzed in triplicate. With these procedures, both C02 and NH3 could be analyzed with an accuracy of about 5%.From the

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A study of the behaviour of some sixty amino-acids and other ninhydrin-reacting substances on phenol-‘collidine’ filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids

Cited by 614 publications

References 11 publications

Homocystinuria: A New Inborn Error of Metabolism Associated With Mental Deficiency

Homocystinuria: A New Inborn Error of Metabolism Associated With Mental Deficiency

Investigations on Nitrogen Compounds and Nitrogen Metabolism in Plants. Ii. Variables in Two-Directional Paper Chromatography of Nitrogen Compounds: A Quantitative Procedure

GLUTAMINE, GLUTAMIC ACID, AND Γ-Aminobutyric ACID IN CEREBROSPINAL FLUIDS

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