In microchannel fluid flow, understanding of the liquid-gas interface behavior is vital for developing a wide range of microfluidic devices. The dynamic contact angle of the liquid-air meniscus varies with its velocity and the ensuing meniscus shape has profound effect on the local transport characteristics in its vicinity. Depending on the application, dynamic menisci shapes eventually control the momentum, heat and mass transport coefficients in two-phase microchannel flow geometries, where such conditions are often encountered. To better understand the effect of dynamic contact angle on meniscus shape, high speed visualization of menisci of four different liquids (water, ethanol, glycerin and silicon oil) has been undertaken at different Capillary numbers. Quantitative information of the velocity field and its distribution near the moving liquid-air interface has been done using micro-PIV measurements in a 1 mm × 1 mm dry square capillary having deionized water as the working fluid. This provides vital information on the local flow transport characteristics (two-dimensional velocity fields on a longitudinal plane) in the wake of the meniscus. To augment and complement the study, three-dimensional simulation of the flow field near the liquid-air meniscus has also been performed on Comsol ® , applying the two-phase flow level-set method. The results clearly demonstrate that, while the u-velocity profile in the liquid domain is parabolic (Poiseuille-type flow) in nature away from the interface, it drastically changes (become flatter) as we approach the meniscus. Close to the meniscus the flow becomes three-dimensional with both v and w velocities showing a double-vortex, the strength of the latter being lower than the former. This observation is clearly noted both by PIV data as well as the simulations. The characteristic of the flow field in the meniscus wake is the most important parameter which affects the viscous stress generated due to the meniscus motion. The study reveals that controlling the wettability of the liquid can be an effective tool to control the overall transport behavior of the moving confined meniscus.