A study of spectrin and lipid peroxidation of red blood cell membrane in thalassaemia carrier

Abstract

The aim of the present work is to understand the lipid peroxidation of RBC membrane and the spectrin protein content of RBC membrane cytoskeleton of thalassaemic carrier state (trait) of 13 and hemoglobin E variant (HbE). We have measured the hemoglobin (Hb), malondialdehyde (MDA) and spectrin content of RBC membrane of thalassaemic carrier. The spectrin content (o~ and 13 band) of both J3 and HbE carrier was not changed than normal individuals. However, lipid peroxidation of RBC membrane was significantly increased in both 13 and HbE trait, and Hb level was also decreased in thalassaemic carrier. It may be assumed that oxidative damage by excess lipid peroxidation may have no role on irreversible membrane damage in 13 thalassaemia and HbE thalassaemia carrier.