A study of single nucleotide polymorphism in the &lt;i&gt;ystB&lt;/i&gt; gene of &lt;i&gt;Yersinia enterocolitica&lt;/i&gt; strains isolated from various wild animal species

Bancerz‐Kisiel, Agata; Szczerba‐Turek, Anna; Platt-Samoraj, A.; Michalczyk, Maria; Szweda, W.

doi:10.5604/12321966.1230737

Cited by 8 publications

(12 citation statements)

References 18 publications

(24 reference statements)

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“…The detected Yersinia enterocolitica was poorly virulent or non-pathogenic; indeed, most isolates harboured only one or none of the investigated virulence genes. The presence of few virulence genes in Yersinia enterocolitica isolates was also reported by other authors, highlighting that wild boar is a reservoir for nonpathogenic or less virulent strains [36,58,59,61]. Furthermore, this finding could be linked to the distribution of the detected biotypes.…”

Section: Discussionsupporting

confidence: 77%

“…In this investigation, the ystA virulence gene was the most prevalent, as demonstrated for other wild boar isolates [62]. Moreover, several wild boar isolates scored positive for the inv [60], ail [59,62,63] and ystB [58,61] virulence genes, as obtained from samples here investigated. virF is a plasmid regulatory gene located on the virulence plasmid designated pYV; the plasmid genes encoded by this plasmid guide the invasion of Yersinia enterocolitica and enable bacteria to survive inside the human host, and for this reasons they are considered essential for pathogenesis [64].…”

Section: Discussionsupporting

confidence: 70%

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Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

et al. 2021

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Wild boar is an animal the population of which constantly increases in Europe. This animal plays an important role as a reservoir for several pathogens, including three of the most important zoonoses: salmonellosis, yersiniosis and listeriosis. The aim of this investigation was to evaluate the occurrence of antimicrobial-resistant and virulence factor genes of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes isolated from wild boar in Tuscany (Central Italy). During two consequent hunting seasons (2018/2019 and 2019/2020), rectal swabs, spleens and livers were collected from 287 hunted wild boar to isolate strains. Each isolate was tested to investigate its antimicrobial resistance and to detect virulence factor genes by PCR. Eighteen Salmonella strains (6.27%) were isolated. Of these, 66.7% were resistant to streptomycin, 13.4% to cephalothin, 6.67% to imipenem and one isolate (6.67%) was resistant simultaneously to five antimicrobials. Moreover, the most detected genes were sopE (73.4%), pipB (66.7%), sodCI (53.3%), spvR and spvC (46.7%). In total, 54 (17.8%) Yersinia enterocolitica were isolated; of them, 26 (48.1%), 9 (16.7%), 17 (31.5%), 1 (1.85%) and 1 (1.85%) belonged to biotypes 1, 2, 3, 4 and 5, respectively. All strains (100%) demonstrated resistance to cephalothin and 70.4% to amoxicillin-clavulanic acid, 55.6% to ampicillin, and 37.0% to cefoxitin. Additionally, the most detected genes were ystA (25.9%), inv (24.1%), ail (22.2%), ystB (18.5%) and virF (14.8%). Finally, only one Listeriamonocytogenes isolate (0.35%) was obtained, belonging to serogroup IVb, serovar 4b, and it was found to be resistant to cefoxitin, cefotaxime and nalidixic acid. The results highlighted the role of wild boar as a carrier for pathogenic and antimicrobial-resistant Salmonella spp., Yersinia enterocolitica and Listeria monocytogens, representing a possible reservoir for domestic animals and human pathogens.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 70%

Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

et al. 2021

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“…The SNP analysis of the ystB gene supported the identification of strains belonging to four genotypes (with 100% homology to the reference gene) and 12 variations (Table 3 ). Variations were defined as sequences not showing 100% homology with the reference sequences previously described by Bancerz-Kisiel et al [ 30 ]. Thirteen sequences were classified as genotype 2; nine sequences as genotype 4; six sequences as genotype 3 and three sequences as genotype 1.…”

Section: Resultsmentioning

confidence: 99%

Isolation, characterization and antimicrobial resistance of Yersinia enterocolitica from Polish cattle and their carcasses

Łada,

Kończyk-Kmiecik,

Bancerz-Kisiel

2023

BMC Vet Res

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Background Yersinia enterocolitica is a heterogeneous bacterial species that has been divided into six biotypes and more than 70 serotypes. Each year, the European Food Safety Authority classifies yersiniosis caused by Y. enterocolitica as one of the most important zoonotic diseases. The prevalence of Y. enterocolitica in cattle has not been thoroughly analyzed in Poland, and beef and bovine carcasses contaminated with antimicrobial resistant Y. enterocolitica pose a health risk for both, farm workers and consumers. Therefore, the aim of this study was to evaluate the prevalence of Y. enterocolitica in cattle and to determine the antimicrobial susceptibility of the isolated strains. Results A total of 1020 samples were analyzed, including 660 rectal swabs collected from live cattle and 360 swabs from cold-stored beef carcasses. The results of this study indicate that Y. enterocolitica was isolated from three of the 15 examined cattle herds and the prevalence within these herds ranged from 0% to nearly 32%. Y. enterocolitica was isolated from 14.7% of the examined heifers, 7.4% of calves and 5.5% of adult cows. More than 65% of the strains were isolated from cold enrichment. The strains isolated from live cattle tested positive for the ystB gene, while ail and ystA genes were not found. Most of the isolated strains belonged to bioserotype 1A/NT. The majority of the isolated strains were resistant to ampicillin, cefalexin and amoxicillin with clavulanic acid, however these are expected phenotypes for Y. enterocolitica. Conclusions The results of this study indicate that Y. enterocolitica is present in cattle herds in Poland. The strains isolated from live cattle were ystB-positive, most of them belonged to bioserotype 1A/NT. The prevalence of Y. enterocolitica strains was generally low in cold-stored beef carcasses.

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“…; Bancerz‐Kisiel et al . ). Furthermore, the specificity of SNPs detection using HRM technology was comparable to DNA sequencing, and overcomes probe‐dependent PCR genotyping methods, as previously reported (Pietzka et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Bancerz‐Kisiel et al . ) and detection of antimicrobial resistance genic variants (Whiley et al . ; Chen et al .…”

Section: Introductionmentioning

confidence: 99%

High resolution melting analysis for the characterization of lineage II Listeria monocytogenes serovars 1/2a and 1/2c based on single nucleotide polymorphisms identification within the Listeria Pathogenicity Island-1 and inlAB operon: a novel approach for

2018

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A study of single nucleotide polymorphism in the <i>ystB</i> gene of <i>Yersinia enterocolitica</i> strains isolated from various wild animal species

Abstract: The proposed HRM method could be used to analyze Y. enterocolitica biotype 1A strains isolated from different sources, including humans.

Cited by 8 publications

References 18 publications

Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

Isolation, characterization and antimicrobial resistance of Yersinia enterocolitica from Polish cattle and their carcasses

High resolution melting analysis for the characterization of lineage II Listeria monocytogenes serovars 1/2a and 1/2c based on single nucleotide polymorphisms identification within the Listeria Pathogenicity Island-1 and inlAB operon: a novel approach for

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