A study of properties and enzymatic hydrolysis of bacterial cellulose

Kashcheyeva, Ekaterina I.; Гладышева, Е. К.; Скиба, Е. А.; Будаева, В. В.

doi:10.1007/s10570-018-02242-7

Cited by 20 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…where l is the particle size determined by granulometric analysis and ρ is the density of the biomass (assumed to be equal to 1.2 g/cm 3 ). The nitrogen sorption/desorption curves were plotted on a Sorptometer M specific surface area analyzer (ZAO Catakon, Novosibirsk, Russia) at a desorption temperature of −5 • C, thermostat temperature of 45 • C, adsorber temperature of −196 • C, heating time of 600 min, and heating rate of 200 • C/min.…”

Section: Matrix Structure and Morphology Analysismentioning

confidence: 99%

“…Cellulose was chosen as the most common plant polysaccharide, β-glucan was chosen as a representative of the second most important component of cell walls-hemicelluloses, konjac gum, and potato starch-as plant raw material polysaccharides that are important for the food industry [1,2]. The biomass surface area accessible for reactions at the solid/liquid interface plays a significant role [3][4][5]. Thus, during heterogeneous enzymatic hydrolysis of cellulose, the initial rate of the reaction (the interaction between enzymes on a solid substrate surface) directly depends on the number of accessible sites (the accessible surface area).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

et al. 2020

View full text Add to dashboard Cite

The surface area is an important parameter in setting any biorefining technology. The aim of this study was to investigate the applicability of sorption of methylene blue to characterize the surface of the main biomass carbohydrates: α-cellulose, sigmacell cellulose, natural gum, β-glucan, and starch. The morphology of particles of the model objects was studied by scanning electron microscopy. Nitrogen adsorption isotherms demonstrate that the selected carbohydrates are macroporous adsorbents. The monolayer capacities, the energy constants of the Brunauer–Emmett–Teller (BET) equation, and specific surface areas were calculated using the BET theory, the comparative method proposed by Gregg and Sing, and the Harkins–Jura method. The method of methylene blue sorption onto biomass carbohydrates was adapted and mastered. It was demonstrated that sorption of methylene blue proceeds successfully in ethanol, thus facilitating surface characterization for carbohydrates that are either soluble in water or regain water. It was found that the methylene blue sorption values correlate with specific surface area determined by nitrogen adsorption/desorption and calculated from the granulometric data. As a result of electrostatic attraction, the presence of ion-exchanged groups on the analyte surface has a stronger effect on binding of methylene blue than the surface area does. Sorption of methylene blue can be used in addition to gas adsorption/desorption to assess the accessibility of carbohydrate surface for binding large molecules.

show abstract

Section: Matrix Structure and Morphology Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The BC network structure was examined by scanning electron microscopy (SEM) using a JSM-840 microscope with a Link-860 Series II X-ray microanalyzer (JEOL, Tokyo, Japan). For this, BC hydrogels were pre-dehydrated with ethanol, freeze-dried, fixed onto a conductive adhesive tape, and sputter-coated with 10 nm of silver for 2 min at 20–30 Å before observation [59].…”

Section: Methodsmentioning

confidence: 99%

Pretreatments of Non-Woody Cellulosic Feedstocks for Bacterial Cellulose Synthesis

2019

Self Cite

View full text Add to dashboard Cite

Pretreatment of biomass is a key step in the production of valuable products, including high-tech bacterial cellulose. The efficiency of five different pretreatment methods of Miscanthus and oat hulls for enzymatic hydrolysis and subsequent synthesis of bacterial cellulose (BC) was evaluated herein: Hydrothermobaric treatment, single-stage treatments with dilute HNO3 or dilute NaOH solution, and two-stage combined treatment with dilute HNO3 and NaOH solutions in direct and reverse order. The performance of enzymatic hydrolysis of pretreatment products was found to increase by a factor of 4−7. All the resultant hydrolyzates were composed chiefly of glucose, as the xylose percentage in total reducing sugars (RS) was 1−9%. The test synthesis of BC demonstrated good quality of nutrient media prepared from all the enzymatic hydrolyzates, except the hydrothermobaric treatment hydrolyzate. For biosynthesis of BC, single-stage pretreatments with either dilute HNO3 or dilute NaOH are advised due their simplicity and the high performance of enzymatic hydrolysis of pretreatment products (RS yield 79.7−83.4%).

show abstract

“…The morphology of substrates, as well as unreacted residues after their hydrolysis in a fermenter, was studied by JSM-840 scanning electron microscopy (SEM) instrument (Jeol, Japan) [32].…”

Section: Ei Kashcheyeva Gf Mironova Vv Budaeva Et Al Bioconmentioning

confidence: 99%

Bioconversion of oat hull and miscanthus cellulose to glucose solutions

Kashcheyeva¹,

Mironova²,

Будаева³

et al. 2019

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY

Self Cite

View full text Add to dashboard Cite

Cellulose-containing raw materials are currently considered to be among the most promising types of raw materials for the production of value-added bio-products. However, for the implementation of the developed process, the application of universal pre-processing methods is a prerequisite. In the present paper, a study into the bioconversion of oat hull and miscanthus cellulose samples by enzymatic hydrolysis into glucose solutions is presented. The substrates were obtained by two-stage processing of raw materials with dilute solutions of nitric acid and sodium hydroxide. Enzymatic hydrolysis was carried out using the Cellolux-A and Ultraflo Core enzyme preparations at an initial substrate concentration of 40 g/L. At the first stage, the reactivity of the substrates to enzymatic hydrolysis in an acetate buffer solution was studied over a period of 72 hours. Cellulose samples from both types of raw materials were established to possess equally high reactivity in terms of the reducing substances. Glucose obtained from the substrate yielded 94-95 % and 88-91 %, respectively. This indicates the universality of the pre-treatment method used for raw materials having a cellulose content of 35-45 %. At the second stage, hydrolysis of the substrates was carried out using a pilot fermenter in an aqueous medium with an excess of enzyme preparations. Over a period of 32 hours, aqueous hydrolysates were obtained with a concentration of reducing substances and glucose equal to 42 g/L (94 % yield from the substrate) and 33-35 g/L (74-78 % yield from the substrate), respectively. The glucose (79-83 %) and pentose (1-2 %) content of the reducing substances indicates glucose to predominate in the composition of the resulting solutions. The high bioconversion e fficiency rate is additionally demonstrated by a comparative analysis of scanning electron spectroscopy results for su bstrates and residues following hydrolysis in a pilot fermenter. Glucose solutions obtained in an aqueous medium are emerging as promising materials for the preparation of culture media and the synthesis of valuable metabolites.

show abstract

A study of properties and enzymatic hydrolysis of bacterial cellulose

Cited by 20 publications

References 41 publications

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

Pretreatments of Non-Woody Cellulosic Feedstocks for Bacterial Cellulose Synthesis

Bioconversion of oat hull and miscanthus cellulose to glucose solutions

Contact Info

Product

Resources

About