A study of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biofilms’ thermal and biodegradable properties reinforced with halloysite nanotubes

Hasan, SM Kamrul; Zainuddin, Shaik; Tanthongsack, J; Hosur, Mahesh; Allen, L. H.

doi:10.1177/0021998318763246

Cited by 21 publications

(19 citation statements)

References 32 publications

(47 reference statements)

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“…Overall, the polymer thermal behavior observed under this study conditions reflected data previously obtained for PHBV [ 50 ], confirming its identity. Nevertheless, the experimental sample was characterized by a double endothermic melting peak, which can be attributed to the presence of two crystalline phases of different sizes, orders, and thickness [ 81 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Raho¹,

Carofiglio²,

Montemurro

et al. 2020

Foods

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In the last decade, the dairy industry underwent a rapid expansion due to the increasing demand of milk-based products, resulting in high quantity of wastewater, i.e., whey and ricotta cheese exhausted whey (RCEW). Although containing high content of nutritional compounds, dairy by-products are still disposed as waste rather being reintroduced in a new production chain, hence leading to environmental and economic issues. This study proposes a new biotechnological approach based on the combination of membrane filtration and fermentation to produce poly-hydroxyalkanoates (PHA), biodegradable bioplastics candidate as an alternative to petroleum-derived plastics. The protocol, exploiting the metabolic capability Haloferax mediterranei to synthesize PHA from RCEW carbon sources, was set up under laboratory and pilot scale conditions. A multi-step fractionation was used to recover a RCEW fraction containing 12.6% (w/v) of lactose, then subjected to an enzymatic treatment aimed at releasing glucose and galactose. Fermentation conditions (culture medium for the microorganism propagation, inoculum size, time, and temperature of incubation) were selected according to the maximization of polymer synthesis, under in-flasks experiments. The PHA production was then tested using a bioreactor system, under stable and monitored pH, temperature, and stirring conditions. The amount of the polymer recovered corresponded to 1.18 g/L. The differential scanning calorimetry (DSC) analysis revealed the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as the polymer synthesized, with a relatively high presence of hydroxyvalerate (HV). Identity and purity of the polymer were confirmed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) and X-ray photoelectron (XPS) spectroscopy analyses. By combining the fractionation of RCEW, one of the most abundant by-products from the agri-food industry, and the use of the halophile Hfx mediterranei, the production of PHBV with high purity and low crystallinity has successfully been optimized. The process, tested up to pilot scale conditions, may be further implemented (e.g., through fed-batch systems) and used for large-scale production of bioplastics, reducing the economical and environmental issues related the RCEW disposal.

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Section: Discussionmentioning

confidence: 99%

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Raho¹,

Carofiglio²,

Montemurro

et al. 2020

Foods

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“…It is clear from results that the swelling degree of pure MC sample has been increased about 2 times by the addition of 1 w/v% PG (MC/PG-1), which could be explained by PG high capacity for water absorption and bonding (Ghasemzadeh and Modiri 2020). In contrast, HNTs incorporation (MC/PG-1/HNTs 3%) signi cantly decreased water uptake (p <0.05) due to acting as a barrier for water absorption by turning the original diffusion path of water into a tortuous path (Hasan et al 2018). Besides, increased cross-linking by the addition of HNTs could inversely result in decreased swelling (Huang et al 2017b; Kumar et al 2019).…”

Section: Degradation and Swelling Of Mc/pg Based Hydrogelsmentioning

confidence: 99%

Thermo-Responsive Hydrogels Based on Methylcellulose/Persian Gum Loaded With Taxifolin Enhance Bone Regeneration: An in Vitro / in Vivo Study

Sajadi-Javan

Varshosaz

Mirian

et al. 2021

Preprint

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In-situ forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25-1.5 w/v% PG and 1-7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs. In vitro release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.

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“…Epoxy polymer resins are preferred as matrix material over other polymers in many high-end applications. Nanoparticles as a secondary reinforcement tend to improve the mechanical and thermal properties of matrix material as well as improve the interfacial bond between fiber and matrix [3]. Various organic and inorganic nanoparticles have been investigated by many researchers [4].…”

Section: Effect Of Salt Fog Spray and Ultraviolet Exposure On The Framentioning

confidence: 99%

Effect of Salt Fog Spray and Ultraviolet Exposure on the Fracture Surface Morphology of Fiber Reinforced Polymer Composites

et al. 2020

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A study of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) biofilms’ thermal and biodegradable properties reinforced with halloysite nanotubes

Cited by 21 publications

References 32 publications

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

Thermo-Responsive Hydrogels Based on Methylcellulose/Persian Gum Loaded With Taxifolin Enhance Bone Regeneration: An in Vitro / in Vivo Study

Effect of Salt Fog Spray and Ultraviolet Exposure on the Fracture Surface Morphology of Fiber Reinforced Polymer Composites

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