A study of peptic activity by means of the double histamine test

A new method for the preparation of urogastrone

“…This step involved some loss of activity but resulted in a preparation containing 1 'dose' per 0-2 mg. They used a form of assay based on the 'two-dose' hista- mine technique (Rivers & Vanzant, 1937) and a 'dose' of urogastrone was defined as that quantity which reduced by 50 % the second response as compared with the first in dogs with vagotomized pouches of the entire stomach, secreting acid at the rate of 30-40 ml. of N/10-HCl/hr, i.e.…”

“…Ug has been found to be suitable for intravenous administration to human subjects, after sterilization by passing through a Seitz filter. A dose equivalent to 8-10 1. of urine (1 mg) intravenously inhibits the response to a subcutaneous injection of histamine in a test of the 'double response' type (Rivers & Vanzant, 1937) (Fig. 5).…”

“…This is probably not due to the presence of histamine, since when Ug was tested on the atropinized guinea-pig ileum it gave only a very small and slow contraction in a bath concentration of 10-5, whereas the gut responded well to histamine in a bath concentration of 4 x 10-9. However, the presence of this vasodilator substance would appear to be of mine technique (Rivers & Vanzant, 1937) and a 'dose' of urogastrone was defined as that quantity which reduced by 50 % the second response as compared with the first in dogs with vagotomized pouches of the entire stomach, secreting acid at the rate of 30-40 ml. of N/10-HCl/hr, i.e.…”