A study of oxidative stress in cervical cancer- an institutional study

Zahra, Kulsoom; Patel, Sandeep; Dey, Tulika; Pandey, Uma; Mishra, Sarita

doi:10.1016/j.bbrep.2020.100881

Cited by 21 publications

(11 citation statements)

References 26 publications

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“…MDA levels were raised in both benign and malignant bone tumors as compared to healthy controls, though the rise in levels was more significant in malignant bone lesions. The above findings are consistent with the existing literature [ 12 , 13 , 14 ]. It is pertinent to mention here that though the levels were increased in both benign and malignant tumors, an increase in levels was not found statistically significant in benign bony lesions (p>0.05).…”

Section: Discussionsupporting

confidence: 94%

Oxidative Stress in Primary Bone Tumors: A Comparative Analysis

Dhupper¹,

Yadav²,

Dahiya³

et al. 2022

Cureus

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Background Bone tumors account for 1% of all cancers and have considerable morbidity and mortality. There is a proposed theory of increased oxidative stress characterized by an increased level of reactive oxygen species (ROS) that disrupts the intracellular reduction-oxidation (redox) balance which has been implicated in various diseases including cancer. The aim of the present study was to measure the levels of oxidant stress and antioxidant mechanism in bone tumors (benign as well as malignant). Methods The study cohort consisted of 42 subjects: 14 malignant bone tumors, 14 benign bone tumors, and 14 healthy controls. Serum Malondialdehyde (MDA) levels were determined to assess oxidative stress while antioxidant status was evaluated using superoxide dismutase (SOD). Results Patients with malignant bone tumors showed a significant increase in plasma MDA levels (p<0.05) while SOD levels were significantly decreased (p<0.05). No significant difference in oxidative damage was noted between both the sarcomas (p>0.05). Conclusions In conclusion, an increase in oxidative stress and a decrease in antioxidant status are observed in bone tumors. Further studies on the manipulation of redox balance in patients with bone tumors can act as a useful approach in early diagnosis or designing management strategies for bone tumors.

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Section: Discussionsupporting

confidence: 94%

Oxidative Stress in Primary Bone Tumors: A Comparative Analysis

Dhupper¹,

Yadav²,

Dahiya³

et al. 2022

Cureus

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“…However, the levels of 8-oxoG and 8-oxodG in patients with cervical carcinoma were significantly higher than those in healthy women. Recent studies have shown that 8-oxodG increased significantly in different stages from SILs (squamous intraepithelial lesion, divided into low-grade and high-grade) to cervical carcinoma, which implied that 8-oxodG had an essential part in the occurrence and development of cervical carcinoma [ 15 , 16 , 17 ]. This may be a mechanism to promote tumorigenesis, or it may be a result of excessive tumor release.…”

Section: Discussionmentioning

confidence: 99%

A study on UHPLC-MS/MS analyses of DNA and RNA oxidative damage metabolites in patients with cervical carcinoma: 8-oxoG in urine as a potential biomarker of cervical carcinoma

Lin

Weng

et al. 2022

Heliyon

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“…The anti-oxidative system protects organisms from the impairment initiated by oxidative stress consequently caused by free radicals. Disparity between the oxidationantioxidation system has been reported in various cancers, including cervical cancer [42,43]. Various reports established a strong association between oxidative stress and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Fisetin Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress and Inflammation in Human Cancer Cells, HeLa

Afroze

Pramodh

Shafarin

et al. 2022

IJMS

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Background: Fisetin, a flavonol profusely found in vegetables and fruits, exhibited a myriad of properties in preclinical studies to impede cancer growth. Purpose: This study was proposed to delineate molecular mechanisms through analysing the modulated expression of various molecular targets in HeLa cells involved in proliferation, apoptosis and inflammation. Methods: MTT assay, flow cytometry, nuclear morphology, DNA fragmentation and Annexin–Pi were performed to evaluate the anti-cancer potential of fisetin. Furthermore, qPCR and proteome profiler were performed to analyse the expression of variety of gene related to cell death, cell proliferation, oxidative stress and inflammation and cancer pathways. Results: Fisetin demonstrated apoptotic inducing ability in HeLa cells, which was quite evident through nuclear morphology, DNA ladder pattern, decreased TMRE fluorescent intensity, cell cycle arrest at G2/M and increased early and late apoptosis. Furthermore, fisetin treatment modulated pro-apoptotic genes such as APAF1, Bad, Bax, Bid and BIK at both transcript and protein levels and anti-apoptotic gene Bcl-2, BIRC8, MCL-1, XIAP/BIRC4, Livin/BIRC7, clap-2/BIRC3, etc. at protein levels to mitigate cell proliferation and induce apoptosis. Interestingly, the aforementioned alterations consequently led to an elevated level of Caspase-3, Caspase-8 and Caspase-9, which was found to be consistent with the transcript and protein level expression. Moreover, fisetin downregulated the expression of AKT and MAPK pathways to avert proliferation and enhance apoptosis of cancer cells. Fisetin treatment also improves oxidative stress and alleviates inflammation by regulating JAK-STAT/NF-kB pathways. Conclusion: Together, these studies established that fisetin deters human cervical cancer cell proliferation, enhances apoptosis and ameliorates inflammation through regulating various signalling pathways that may be used as a therapeutic regime for better cancer management.

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A study of oxidative stress in cervical cancer- an institutional study

Cited by 21 publications

References 26 publications

Oxidative Stress in Primary Bone Tumors: A Comparative Analysis

Oxidative Stress in Primary Bone Tumors: A Comparative Analysis

A study on UHPLC-MS/MS analyses of DNA and RNA oxidative damage metabolites in patients with cervical carcinoma: 8-oxoG in urine as a potential biomarker of cervical carcinoma

Fisetin Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress and Inflammation in Human Cancer Cells, HeLa

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