A study of optimal concentration range and time window of sevoflurane preconditioning for brain protection in MCAO rats

Abstract

Background: Sevoflurane preconditioning improves brain function in MCAO rats, and there are several methods for determining appropriate concentration and time windows for preconditioning. This study investigated the brain protective effects with a single sevoflurane preconditioning at different concentrations and different time windows on MCAO rats. Methods: Adult Sprague-dawley rats were randomly assigned to 14 groups. The rats in the sevoflurane preconditioning group inhaled 0.5 MAC, 1.0 MAC, and 1.3 MAC sevoflurane, respectively for 3 h, and then MCAO models were established at 6 h, 12 h, 24 h, and 48 h. MCAO and sham groups underwent no preconditioning with sevoflurane. The neurological severity score, cerebral infarct volume and brain water content of the rats were measured 24 h after reperfusion. Results: After inhalation of 1.3 MAC sevoflurane for 3 h of preconditioning, the MCAO model was established after 24 h. This preconditioning improved the neurological severity score, reduce cerebral infarct volume and brain water content in MCAO rats. After inhalation of 1.0 MAC sevoflurane for 3 h of preconditioning, MCAO model established after 24 h reduced the cerebral infarct volume and brain water content of MCAO rats, but the neurological severity score showed no significant improvement, and no significant brain protective effects were observed at other concentrations and time windows. Conclusions: These results suggested that after inhalation of 1.3 MAC sevoflurane for 3 h of preconditioning, MCAO model established after 24 h demonstrated significant brain protective effects in MCAO rats.

Protective effect of cilostazol on vascular injury in rats with acute ischemic stroke complicated with chronic renal failure

“…Antibiotics were used to prevent incision infection in each group during the procedure. After the CRF rats were anesthetized, as described in a previous study [ 14 ], a nylon thread was prepared, and the external and common carotid arteries were ligated using a silk thread. The prepared nylon cord was then inserted along the common carotid artery into the left internal carotid artery, the vascular clamp was released, the threaded plug was advanced to obstruct the ipsilateral middle cerebral artery, and the threaded plug was fixed above the incision of the left common carotid artery to complete the modeling of MCAO in rats.…”